Angiotensin-converting enzyme 2-based biosensing modalities and devices for coronavirus detection

Gul, Ijaz; Zhai, Shiyao; Zhong, Xiaoyun; Chen, Qun; Yuan, Xi; Du, Zhicheng; Chen, Zhenglin; Yu, Dongmei; Qin, Peiwu

doi:10.31219/osf.io/afg6r

Cited by 3 publications

(1 citation statement)

References 104 publications

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“…To the best of our knowledge, the MPXV entry receptor is still unknown; the discovery of the MPXV entry receptor and the development of MPXV sensors based on the entry receptor could be useful future developments. Novel MPXV biosensors could be developed based on photonics [ 95 ], quantum dots [ 96 ], electrochemiluminescence [ 97 ], electrochemical transduction, lab on a chip [ 30 ], CRISPR technology [ 98 , 99 ], and other approaches [ 100 , 101 , 102 , 103 ]. Introducing smart diagnostic systems based on WWE is anticipated to be a good future work to detect asymptomatic cases.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Current and Perspective Sensing Methods for Monkeypox Virus

et al. 2022

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The outbreak of the monkeypox virus (MPXV) in non-endemic countries is an emerging global health threat and may have an economic impact if proactive actions are not taken. As shown by the COVID-19 pandemic, rapid, accurate, and cost-effective virus detection techniques play a pivotal role in disease diagnosis and control. Considering the sudden multicountry MPXV outbreak, a critical evaluation of the MPXV detection approaches would be a timely addition to the endeavors in progress for MPXV control and prevention. Herein, we evaluate the current MPXV detection methods, discuss their pros and cons, and provide recommended solutions to the problems. We review the traditional and emerging nucleic acid detection approaches, immunodiagnostics, whole-particle detection, and imaging-based MPXV detection techniques. The insights provided in this article will help researchers to develop novel techniques for the diagnosis of MPXV.

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Section: Conclusion and Prospectsmentioning

confidence: 99%

Current and Perspective Sensing Methods for Monkeypox Virus

et al. 2022

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Unraveling the Dynamics of SARS-CoV-2 Mutations: Insights from Surface Plasmon Resonance Biosensor Kinetics

Nurrohman,

Chiu

2024

Biosensors

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Surface Plasmon Resonance (SPR) technology is known to be a powerful tool for studying biomolecular interactions because it offers real-time and label-free multiparameter analysis with high sensitivity. This article summarizes the results that have been obtained from the use of SPR technology in studying the dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations. This paper will begin by introducing the working principle of SPR and the kinetic parameters of the sensorgram, which include the association rate constant (ka), dissociation rate constant (kd), equilibrium association constant (KA), and equilibrium dissociation constant (KD). At the end of the paper, we will summarize the kinetic data on the interaction between angiotensin-converting enzyme 2 (ACE2) and SARS-CoV-2 obtained from the results of SPR signal analysis. ACE2 is a material that mediates virus entry. Therefore, understanding the kinetic changes between ACE2 and SARS-CoV-2 caused by the mutation will provide beneficial information for drug discovery, vaccine development, and other therapeutic purposes.

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Strategies for Increasing the Throughput of Genetic Screening: Lessons Learned from the COVID-19 Pandemic within a University Community

Miguel,

Baleizão,

Gomes

et al. 2024

BioTech

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Amidst the COVID-19 pandemic, the Polytechnic University of Setúbal (IPS) used its expertise in molecular genetics to establish a COVID-19 laboratory, addressing the demand for community-wide testing. Following standard protocols, the IPS COVID Lab received national accreditation in October 2020 and was registered in February 2021. With the emergence of new SARS-CoV-2 variants and safety concerns for students and staff, the lab was further challenged to develop rapid and sensitive diagnostic technologies. Methodologies such as sample-pooling extraction and multiplex protocols were developed to enhance testing efficiency without compromising accuracy. Through Real-Time Reverse Transcription Polymerase Chain Reaction (RT-qPCR) analysis, the effectiveness of sample pooling was validated, proving to be a clear success in COVID-19 screening. Regarding multiplex analysis, the IPS COVID Lab developed an in-house protocol, achieving a sensitivity comparable to that of standard methods while reducing operational time and reagent consumption. This approach, requiring only two wells of a PCR plate (instead of three for samples), presents a more efficient alternative for future testing scenarios, increasing its throughput and testing capacity while upholding accuracy standards. The lessons learned during the SARS-CoV-2 pandemic provide added value for future pandemic situations.

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Angiotensin-converting enzyme 2-based biosensing modalities and devices for coronavirus detection

Cited by 3 publications

References 104 publications

Current and Perspective Sensing Methods for Monkeypox Virus

Current and Perspective Sensing Methods for Monkeypox Virus

Unraveling the Dynamics of SARS-CoV-2 Mutations: Insights from Surface Plasmon Resonance Biosensor Kinetics

Strategies for Increasing the Throughput of Genetic Screening: Lessons Learned from the COVID-19 Pandemic within a University Community

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