Angiotensin converting enzyme in cerebrospinal fluid

Abstract

Serum and CSF angiotensin converting enzyme (ACE) were measured by a new inhibitor binding assay in 32 patients with sarcoidosis, 49 with neurologic diseases, and 38 controls. In neurosarcoidosis, 11 of 20 patients had high levels of CSF ACE. In systemic sarcoidosis without neurologic abnormality, only 1 of 12 patients had elevated CSF ACE. The highest value was observed in a patient with widespread meningeal sarcoidosis. High values were also observed in patients with bacterial meningitis or malignant tumors of the CNS. Fluctuation in successive analyses correlated to clinical course of neurosarcoidosis. CSF ACE analysis seems useful in diagnosis and follow-up of neurosarcoidosis.

Enzima conversora de angiotensina no líquido pericárdico: estudo comparativo com a atividade sérica

“…The molecular mass (140 kDa) obtained through polyacrylamide gel electrophoresis for the isolated isoform, showed to be different when compared to literature data, where molecular masses of 90-110 and 150-180 kDa are described [12][13][14] . This difference could be due to the post-translational modifications, such as polypeptide chain cleavages and/or glycosylation in the isoform of the pericardial fluid.…”

“…Another isoform with a lower molecular mass (90 -110 kDa), found exclusively in male germinative cells, contain a single active site that corresponds to the C-domain of the somatic form 13 . Soluble forms of the enzyme are found in several body fluids, such as blood, amniotic fluid, semen, cerebrospinal fluid and urine [14][15][16] . A form constituted only of the N-domain was described in the ileal fluid 17 .…”

Clinical features of spinal cord sarcoidosis: analysis of 17 neurosarcoidosis patients

“…Therefore, indicators that represent the disease activity of spinal cord sarcoidosis are necessary for reasonable treatment. Oksanen et al reported the usefulness of ACE levels in CSF measured by an inhibitor binding assay to evaluate the disease activity of neurosarcoidosis [18]; however, colorimetry was used in the present case, while investigation by Varron et al demonstrated that spinal cord sarcoidosis patients did not exhibit sufficient changes in the CSF-ACE levels to serve as an indicator.…”

Neurosarcoidosis: Two case reports with multiple cranial nerve involvement and review of the literature

“…Some authors regard ACE levels in CSF as being useful in both the diagnosis and follow-up of NS (ref. 26,27 ). However, many authorities now regard this test as non-specific and insensitive.…”

“…Furthermore, increases in the concentration of ACE (ref. 26,27,[33][34][35][36][37] ), IgG-index [38][39][40] , oligoclonal bands 38,39,41 , CD4/CD8 lymphocyte ratios 42 , lysosymes and beta 2-microglobulin concentrations 43 in CSF have been reported. Most studies report evidence of intrathecal synthesis of immunoglobulins with oligoclonal bands in about 30-40% of patients with NS (ref.…”