Angiotensin II type 1 receptor participates in extracellular matrix production in the late stage of remodeling after vascular injury

Abstract

ATR1 is upregulated in the late stage of remodeling after vascular injury and is important in ECM production.

The Long-Term Effect of Angiotensin II Type 1a Receptor Deficiency on Hypercholesterolemia-Induced Atherosclerosis

“…Reverse-transcription polymerase chain reaction (RT-PCR) was performed as described previously ( 17 ). Total RNA for use in RT-PCR was isolated using the Mini RNA isolation kit (Cat.#R1005, ZYMO RESEARCH, Orange, USA).…”

“…For Western blotting of collagen type I and elastin, proteins were extracted from apoE-KO and D-KO aortas as reported previously (17). The aorta was ground to a fine powder under liquid nitrogen and incubated in ice cold 0.1% Triton lysis solution (10 mmol/L HEPES [pH 7.4], 50 mmol/L sodium pyrophosphate, 50 mmol/L NaF, 5 mmol/L EDTA, 5 mmol/L EGTA, 50 mmol/L NaCl, 100 mmol/L Na3VO4, 0.1% Triton X-100, 500 mmol/L PMSF, and 10 mg/mL leupeptin) for 30 min.…”

“…Western blotting was performed with a NuPAGE™ Electrophoresis System (Cat.#NPO322BOX, Invitrogen) as reported previously (17). Briefly, 10-μg protein samples were resuspended in reduced sample buffer, electrophoresed on a 4-12% Bis-Tris gel (Invitrogen) with MOPS running buffer, blotted to nitrocellulose membrane, and sequentially probed with a goat polyclonal antibody against human collagen type I (Cat.#sc-27954, Santa Cruz Biotechnology), a goat polyclonal antibody against elastin (Cat.#sc-17581, Santa Cruz Biotechnology), a goat polyclonal antibody against human p22phox (Cat.#sc-11712, Santa Cruz Biotechnology), a goat polyclonal antibody against mouse SAP (Cat.#sc-18312, Santa Cruz Biotechnology), a goat polyclonal antibody Fig.…”

ANG II-induced neointimal growth is mediated via cPLA2- and PLD2-activated Akt in balloon-injured rat carotid artery

“…The finding that the AT 1 R antagonists TCV-116 (1, 16 -18, 43) and losartan (14,28,33,46,47,54) inhibit intimal thickening of rat balloon-injured carotid arteries links ANG II to neointimal growth during injury. The AT 1 R is overexpressed in injured vessels (7). This observation, together with our demonstration that the exposure of balloon-injured rat carotid arteries to ANG II increased neointimal growth, which was inhibited by treatment with the adenovirus containing the kinase-dead Akt but not the WT Akt mutant, suggests that Akt activation mediates the neointimal thickening caused by ANG II in injured carotid arteries.…”

Ação da angiotensina II no remodelamento da matriz extracelular perivascular em camundongos.

“…No entanto, em artérias de resistência, a elastina é o componente menos abundante (GONZÁLEZ et al, 2005), embora muito importante no estabelecimento de suas propriedades elásticas, no remodelamento e na progressão da hipertensão, aterosclerose e outras doenças cardiovasculares (BRIONES et al, 2003;FAURY et al, 2003;GONZÁLEZ et al, 2005). Portanto, a resposta celular à fibronectina é complexa e pode depender do contexto da sinalização local (LARSEN et al, 2006).…”