Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells

Wang, Xinhao; Zhang, Hongpeng; Ge, Yangyang; Liu, Jie; Rong, Dan; Cao, Long; He, Yuan; Sun, Guoyi; Jia, Senhao; Guo, Wei

doi:10.3892/etm.2019.8259

Cited by 4 publications

(10 citation statements)

References 29 publications

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“…In contrast, in human aortic endothelial cells, Wang et al reported that AngII stimulation suppressed total YAP and increased p-YAP, as observed in other diseased conditions. 25 The contrary observation of increased total and p-YAP in our present study could be due to a SMC-specific YAP regulation by AngII. In support of our observation, an earlier study by Lin et al demonstrated that AngII infusion increased both total and phospho-YAP in the right common carotid artery in hypertensive rats and cultured rat thoracic aorta SMCs via AT1R activation.…”

Section: Discussioncontrasting

confidence: 96%

Mst1/2 Kinases Inhibitor, XMU-MP-1, Attenuates Angiotensin II-Induced Ascending Aortic Expansion in Hypercholesterolemic Mice

et al. 2021

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Background: Ascending and abdominal aortic aneurysms (AAs) are asymptomatic, permanent dilations of the aorta with surgical intervention as the currently available therapy. Hippo-Yap signaling cascade plays a critical role in stem cell self-renewal, tissue regeneration and organ size control. By using XMU-MP-1, a pharmacological inhibitor of the key component of Hippo-Yap signaling, MST1/2, we examined the functional contribution of Hippo-Yap in the development of AAs in Angiotensin II (AngII)-infused hypercholesterolemic mice.Methods and Results: MST, p-MST, p-YAP, p-MOB and TAZ proteins in AngII-infused ascending and abdominal aortas were assessed by immunohistochemical and western blot analyses. To examine the effect of MST1/2 inhibition on AAs, western diet-fed low density lipoprotein (LDL) receptor −/− mice infused with AngII were administered with either vehicle or XMU-MP-1 for 5 weeks. Hippo-YAP signaling proteins were significantly elevated in AngII infused ascending and abdominal aortas. XMU-MP-1 administration resulted in the attenuation of AngII-induced ascending AAs without influencing abdominal AAs and aortic atherosclerosis. Inhibition of Hippo-YAP signaling also resulted in the suppression of AngII-induced matrix metalloproteinase 2 (MMP2) activity, macrophage accumulation, aortic medial hypertrophy and elastin breaks in the ascending aorta. Conclusions:The present study demonstrates a pivotal role for the Hippo-YAP signaling pathway in AngII-induced ascending AA development.

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Section: Discussioncontrasting

confidence: 96%

Mst1/2 Kinases Inhibitor, XMU-MP-1, Attenuates Angiotensin II-Induced Ascending Aortic Expansion in Hypercholesterolemic Mice

et al. 2021

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“…Cells were inoculated with three replicate sets per experimental group. We followed the CCK-8 method in our previous study (Wang et al, 2020).…”

Section: Cck-8 Assay and Flow Cytometric Analysismentioning

confidence: 99%

“…Endothelin (ET)-1 levels in supernatant from each group at the end of the 96 h treatment and in the mice serum from different group after 4 week feed were tested through competitive inhibition ELISA with ET-1 Antibody ELISA kit (USCN Life Sciences, Inc.), and interleukin (IL)-6 and matrix metallopeptidase (MMP)-9 in the cell solutions and mice serum were detected by double antibody ELISA with IL-6 ELISA Kit (USCN Life Sciences, Inc.) and MMP9 ELISA Kit (USCN Life Sciences, Inc.), respectively. We follow our previous research for ELISA (Wang et al, 2020).…”

Section: Elisa For Et-1 Il-6 and Mmp-9 Levels In The Supernatantmentioning

confidence: 99%

“…ab9485; polyclonal, rabbit anti-human and mouse; 1:2,500; Abcam). The specific operations and reagents are the same as our previous research (Wang et al, 2020). ImageJ (V1.8.0, National Institutes of Health) was used to evaluate the density of bands by quantitative densitometry.…”

Section: Western Blotting Forat1r and Yapmentioning

confidence: 99%

“…Shanghai GenePharma Co., Ltd., designed and synthesized AT1R siRNA as previous study (Wang et al, 2020). YAP siRNA was also designed and synthesized be the same company as follows: YAP -860, GCAUCUUCGACAGUCUUCUTT; YAP -941, GGUCAGAGAUACUUCUUAATT; YAP -1662, GGUGAUACUAUCAACCAAATT; NC, UUCUCCGAACGUG UCACGUTT.…”

Section: At1r Sirna and Yap Sirna Transfectionmentioning

confidence: 99%

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AT1R Regulates Macrophage Polarization Through YAP and Regulates Aortic Dissection Incidence

Wang

Zhang

et al. 2021

Front. Physiol.

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Aortic dissection (AD) is one of the most fatal cardiovascular emergency. At the anatomical level, AD occurs due to the formation of intimal tears. However, the molecular mechanism underlying this phenomenon remains unknown. Angiotensin II (Ang II) is a important effector in the development of cardiovascular disease that acts through binding to angiotensin type 1 receptor (AT1R). Yes-associated protein (YAP) was recently recognized as a key protein in macrophage activation. To determine whether AT1R and YAP are involved in macrophage-induced endothelial cell (EC) inflammation and AD incidence, we co-cultured THP-1 cells and HAECs in transwell chambers under different culture conditions and apply different conditions to the AD mice model. The results showed that Ang II promoted macrophage M1 polarization and adhesion, upregulated YAP phosphorylation, and induced EC injury that was related to increased levels of multiple pro-inflammatory chemokines. Blocking AT1R function pharmacologically or by transfection with AT1R siRNA can reduce the pro-inflammatory effect induced by Ang II. In addition, siRNA knock down of YAP expression further aggravated the pro-inflammatory effects of Ang II. Treatment with ARB effectively alleviated these pro-inflammatory effects. In the mice AD model, ARB effectively reduced the incidence of AD in mice, decreased M1 macrophages infiltration and AT1R content in the aortic wall and increased the tissue content of YAP. We found that AT1R induces YAP phosphorylation through binding to Ang II, and further promotes macrophage M1 polarization and adhesion to ECs. ARB reduces the incidence of AD in mice and affect macrophage polarization in mice aorta.

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Association between elevated endocan levels and obstructive sleep apnea hypopnea syndrome in Turkey and China: A meta-analysis

He,

Li,

et al. 2023

Medicine

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Background: Endothelial dysfunction has been implicated in the pathogenesis of obstructive sleep apnea–hypopnea syndrome (OSAHS). Previous research has linked endothelial dysfunction to the vascular pathology marker endothelial cell-specific molecule-1 (endocan). This meta-analysis compared endocan concentrations among adult patients with OSAS and controls, and ascertained whether any differences exist. This study aimed to highlight the association between high endocan levels and OSAHS. Methods: A comprehensive, systematic literature search of the PubMed, Cochrane Library, China National Knowledge Infrastructure, Web of Science, Embase, and Wan Fang databases for relevant studies, published between January 2000 and June 10, 2013, was performed. Additionally, standardized mean differences, correlation coefficients, and adjusted odds ratios were used to assess the effect size. Statistical analyses were performed using R version 4.13 (Copenhagen: The Cochrane Collaboration) and Stata version 10.0 (StataCorp LLC, College Station, TX). Results: Twelve studies fulfilled the inclusion criteria. Nine studies reported endocan levels in patients with OSAHS and controls, and 6 reported serum endocan levels in relation to polysomnography (PSG) indexes (apnea-hypopnea index, body mass index, minimum oxygen (O2) saturation, and flow-mediated dilatation [FMD]). Five studies reported that serum endocan levels functioned independently as risk factors for OSAHS. These levels were determined to be elevated in adults with OSAHS compared with controls (standardized mean difference 1.30 [95% confidence interval (CI) 1.06–1.54]) and increased more significantly with increasing disease severity in individuals with OSAHS. Subjects were divided into different subgroups based on race, geographical region, sample type, and study design. Results indicated increased endocan levels across all OSAHS subgroups compared with the control group. The data highlighted a positive association between serum endocan levels and apnea-hypopnea index, and a negative association with FMD and minimum O2 saturation. The overall adjusted odds ratio between serum endocan levels and OSAHS was 1.04 (95% CI 1.02–1.06). Conclusion: Results of this meta-analysis provide further evidence supporting elevated endocan levels in adults with OSAHS. Serum endocan levels were correlated with various PSG indices and may be associated with OSAHS.

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Angiotensin type 1 receptor regulates yes‑associated protein in vascular endothelial cells

Cited by 4 publications

References 29 publications

Mst1/2 Kinases Inhibitor, XMU-MP-1, Attenuates Angiotensin II-Induced Ascending Aortic Expansion in Hypercholesterolemic Mice

Mst1/2 Kinases Inhibitor, XMU-MP-1, Attenuates Angiotensin II-Induced Ascending Aortic Expansion in Hypercholesterolemic Mice

AT1R Regulates Macrophage Polarization Through YAP and Regulates Aortic Dissection Incidence

Association between elevated endocan levels and obstructive sleep apnea hypopnea syndrome in Turkey and China: A meta-analysis

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