Anhang 1

Mulisch, Maria; Welsch, Ulrich

doi:10.1007/978-3-642-55190-1_26

Cited by 63 publications

(96 citation statements)

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“…Serial sections of a pad revealed that the substance is mainly present in the central part of the contact zone and diminishes toward its periphery. Its yellow staining with FCA indicates the presence of cutin (Etzold 2002;Mulisch and Welsch 2010). To specify the chemical nature of the substance, further histochemical analyses were carried out.…”

Section: Bohn Et Al-attachment Of Adhesive Tendrils In Passiflora DImentioning

confidence: 99%

“…Sections were stained in fuchsin-chrysoidin-Astra-blue solution (FCA: 1000 mL distilled water, 0.1 g new fuchsin, 0.143 g chrysoidin, 1.25 g Astra blue, 20 mL acetic acid) for 6.5 min, followed by removal of the dye in ethanol (30% for 10 s, 70% for 15 s, 30% for 15 s), and finally rinsed with isopropyl alcohol. Lignin is stained red, cellulose blue, and cutin yellow-orange (Etzold 2002;Mulisch and Welsch 2010). Pads grown on corkboards and into cavities were carefully cut out with a broad amount of cork.…”

Section: Lm and Stainingmentioning

confidence: 99%

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A Passionate Free Climber: Structural Development and Functional Morphology of the Adhesive Tendrils in Passiflora discophora

Bohn

Günther

Fink

et al. 2015

International Journal of Plant Sciences

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Editor: Adrienne NicotraPremise of research. Passiflora discophora is exceptional among passion flowers for its climbing strategy, using branched tendrils with terminal adhesive pads instead of coiling tendrils as typical within this family. This article investigates the structural development and morphology of these adhesive pads and aims to understand the underlying structure-function relationship of the attachment process. Based on our results, we discuss possible mechanical consequences of the tendril structure and compare our findings with similar attachment systems in unrelated species in order to identify general strategies of this mode of attachment.Methodology. We investigated the temporal development of the attachment process, including detailed studies of the morphology and anatomy of the adhesive pads, using LM with different staining procedures and SEM.Pivotal results. Young tendrils establish initial contact with a supporting substrate by interlocking with their hook-shaped tips. Touch stimuli induce the tips to develop into adhesive pads by callus-like growth of papillate epidermal cells. Fully grown pads are hemispherical on flat substrates or completely fill out larger cavities of the substrate. By apical cell division, the pad tissue perfectly mimics the microtopography of the substrate and also grows into minute gaps and fissures, establishing firm anchorage by optimal form closure. Additionally, an extracellular substance is visible at the interface between pad and substrate surface, which might act as adhesive.Conclusions. The opportunistic growth and cellular structure make the tendrils and adhesive pads of P. discophora a highly adaptive attachment system. Comparison with other not closely related taxa reveals general principles of this climbing mode, namely, (1) branched tendrils with multiple adhesive pads, (2) papillate cells establishing optimal form closure with the substrate and in some species additionally secreting adhesive substances, (3) free coiling of axes, and (4) persisting anchorage after senescence.

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Section: Bohn Et Al-attachment Of Adhesive Tendrils In Passiflora DImentioning

confidence: 99%

Section: Lm and Stainingmentioning

confidence: 99%

A Passionate Free Climber: Structural Development and Functional Morphology of the Adhesive Tendrils in Passiflora discophora

Bohn

Günther

Fink

et al. 2015

International Journal of Plant Sciences

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“…Sections of all stages were stained with haematoxylin-eosin (HE) and Goldner according to the methods of Romeis and Böck [1989]. Tissue preparation for light microscopy and immunohistochemistry were according to the methods of Mulisch and Welsch [2010] and were described already in earlier publications Sinowatz, 2007, 2008;Kenngott et al 2011].…”

Section: Tissue Samples and Preparationmentioning

confidence: 99%

The Role of Ovarian Surface Epithelium in Folliculogenesis during Fetal Development of the Bovine Ovary: A Histological and Immunohistochemical Study

Kenngott

Vermehren

Ebach

et al. 2013

Sex Dev

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Although many aspects of ovarian differentiation have been established, comparatively little is known about prenatal follicle formation and differentiation of bovine ovaries. The objective of this investigation was to study the role of the surface epithelium during the development of germ cell nests, germ cell cords and follicle formation in the fetal bovine ovary. Associated important proliferation and apoptotic features were further investigated. Additionally, the expression pattern of the S100 protein was detected. A strong increase of mitotic figures was detected in the surface epithelium, germ cell nests and germ cell cords of ovaries with a crown-rump length (CRL) of 13.0-58.0 cm. Oocytes were positively stained with S100 in bovine ovaries from fetuses with a CRL of 21.0 cm. The staining intensity enhanced parallel to increasing oocyte and follicle sizes during the ovary development. In later stages, a strong staining for S100 was observed in healthy oocytes in contradistinction to atretic oocytes where no expression of the S100 protein could be found. In conclusion, increasing mitosis index of surface epithelium cells, as well as oogonia directly beneath the surface epithelium, in combination with open surface connection during stages from a CRL of 11.0-94.0 cm of bovine fetal ovaries could play an important role in the period of time of ongoing folliculogenesis and derivation of granulosa cells. Additionally, S100-positive oocytes in primordial and later follicle stages joined by a high rate of Ki67-positive index in surrounding granulosa cells indicate that in the oocytes the S100 protein can perhaps be a useful marker for intact oocytes in bovine ovaries.

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“…16 For IHC serial sections (3 µm) of skin and hoof, tissues were mounted on 3-aminopropyltriethoxysilane/glutaraldehyde -coated slides. Endogenous peroxidase activity was blocked by incubation in 0.6% H 2 O 2 in methanol for 15 min at room temperature.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

Equine Hoof Canker: Cell Proliferation and Morphology

et al. 2017

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Hoof canker is described as progressive pododermatitis of the equine hoof with absent epidermal cornification and extensive proliferation of the dermal papillary body; however, in-depth research on the type of proliferative activity has not yet been reported. The aim of the present study was to determine cell-specific proliferation patterns together with morphological analysis of hoof canker tissue.Tissues removed during surgery from 19 horses presented for treatment of canker were compared to similar postmortem tissues of healthy hooves of 10 horses.Morphological alterations visible in light microscopy were assessed semiquantitatively and graded for severity. Proliferative activity was evaluated by means of anti-PCNA (proliferative cell nuclear antigen) and anti-Ki67 immunohistochemistry.Histologically, canker tissue showed five major morphological alterations, the presence of lacunae, vacuoles, giant cells, hemorrhage and inflammation, not seen in control tissue. Also, there was a notable koilocytotic appearance of keratinocytes in canker tissue. Immunohistochemistry revealed increased levels of PCNA protein expression in keratinocytes and fibroblasts of canker tissue compared to control tissue. In control tissue, keratinocytes showed higher levels of Ki67 compared to canker tissue, while the dermal fibroblasts of both groups showed similar levels of Ki67, indicating similar proliferative activity of less than 3% of total dermal fibroblasts.These results demonstrate that, in contrast to previous reports, there is no evidence for increased proliferative activity of the dermal papillary body associated with hoof canker. Increased levels of PCNA protein expression and morphological alterations indicate dysregulation of keratinocyte differentiation constitutes a key event in equine hoof canker development. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 3,9,19,20,28 However, for any of these, a causal association with the development of disease remains to be established. Morphological studies of hoof canker tissues are rare. 24, 26Ballooning of the keratinocytes, mainly in the Stratum germinativum of the frog, has been reported together with the occurrence of perinuclear vacuoles in the cells of deep layers of the Stratum spongiosum; the cells of the corium are covered by a thin layer of defective epidermis, leading to an increased risk of infection and bleeding. 26To our knowledge, the proliferative character of canker has not yet been analyzed in detail. Immunohistochemistry allows characterization of tissue proliferation, as has been shown in a number of potentially metaplastic or neoplastic tissues. 4,1...

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Anhang 1

Cited by 63 publications

References 0 publications

A Passionate Free Climber: Structural Development and Functional Morphology of the Adhesive Tendrils in Passiflora discophora

A Passionate Free Climber: Structural Development and Functional Morphology of the Adhesive Tendrils in Passiflora discophora

The Role of Ovarian Surface Epithelium in Folliculogenesis during Fetal Development of the Bovine Ovary: A Histological and Immunohistochemical Study

Equine Hoof Canker: Cell Proliferation and Morphology

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