Animal models for the assessment of acute renal dysfunction and injury

Angiotensin II facilitates autoregulation in the perfused mouse kidney: An optimized in vitro model for assessment of renal vascular and tubular function

“…When simpler surgery and improved perfusion solutions were introduced by Ross, the model became useful for studies of renal biochemistry 7 . With further improvements, including the addition of amino acids 9 and sometimes erythrocytes, 5,14 the model became useful for studies of physiology and pathophysiology 8,15–19 . We have now optimized the isolated perfused mouse kidney model for the simultaneous study of vascular, tubular and glomerular function.…”

“…Isolating the kidney from neurohumoral influences allows tight control of many physiological parameters and provides opportunities for the studying of physiological and biochemical control mechanisms. The IPMK should be similarly useful for investigating renal ischemia‐reperfusion, hypoxic and toxic injury 19 . However, with the advances in molecular genetics over the last decade, the mouse has become the animal model of choice for studying the genetic basis of many diseases.…”

“…7 With further improvements, including the addition of amino acids 9 and sometimes erythrocytes, 5,14 the model became useful for studies of physiology and pathophysiology. 8,[15][16][17][18][19] We have now optimized the isolated perfused mouse kidney model for the simultaneous study of vascular, tubular and glomerular function. Autoregulation, under baseline conditions, was limited in both range (65-85 mmHg) and efficiency (ARI: 0.66 ± 0.11) as has been observed in perfused kidneys from Sprague-Dawley rats.…”

“…The IPMK should be similarly useful for investigating renal ischemia-reperfusion, hypoxic and toxic injury. 19 However, with the advances in molecular genetics over the last decade, the mouse has become the animal model of choice for studying the genetic basis of many diseases. Our preliminary results suggest that this model could have novel and useful applications in the study of renal tubular metabolism, molecular pathology and in vascular regulation when applied to kidneys from mice with selected gene deletions.…”