Animal models for the study of tendinopathy

Warden, Stuart J.

doi:10.1136/bjsm.2006.032342

Cited by 132 publications

(114 citation statements)

References 97 publications

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…Currently, rabbit Mesenchymal Stem Cells (rMSCs) are very popular among researchers due to their resemblance in cellular and tissue physiology with that of human MSCs (Fox, 1984;Warden, 2007) and due to their easy availability. As per the International Society for Cell Therapy (ISCT), cells that are plastic adherent, express CD105, CD73 and CD90, lack expression of CD45, CD34, CD14 or CD11b, CD79a or CD19 and HLA-DR surface molecules and can differentiate into osteogenic, chondrogenic and adipogenic lineages are described as Mesenchymal Stem Cells (MSCs) (Dominici et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is imperative to isolate, culture, expand and characterize MSCs before their effective use. Currently, rabbit mesenchymal stem cells are very popular among researchers due to their resemblance in cellular and tissue physiology with that of human MSCs (Fox, 1984;Warden, 2007) and due to their easy availability. To evaluate stem cells, it is important to have uninterrupted source of such cells that too with minimal ethical issues.…”

Section: Introductionmentioning

confidence: 99%

“…Rabbits are readily available with larger size compared to mouse or rat and easy to handle and cost effective in comparison to dog, sheep or goat (Tan et al, 2013). However, the literature about the basic characteristics of rMSCs is not that much extensive as that of the human Mesenchymal Stem Cells (hMSCs) (Warden, 2007;Amini et al, 2012). So the present study was aimed at culturing, characterization and differentiation of rMSCs.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

Gugjoo¹,

Amarpal²,

Kinjavdeka³

et al. 2015

Asian J. of Animal and Veterinary Advances

View full text Add to dashboard Cite

Mesenchymal stem cells are recognised based upon the plastic adherence, fibroblastic morphology, expression of certain surface markers, non-expression of haematopoetic markers and their ability to differentiate into atleast three lineages viz., adipogenic, chondrogenic and osteogenic. The rabbit Mesenchymal Stem Cells (rMSCs) though used extensively in research but have not been thoroughly studied and are not compared to other species. The present study was therefore conducted to determine the morphology, surface markers and trilineage differentiation potential of New Zealand white rabbit MSCs. Isolation of rMSCs was done by an established method of density gradient method using Ficoll-hapaque. The cells were characterised by Phase Contrast Microscopy, Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Alkaline Phosphatase (AP) staining. The cells isolated were plastic adherent and had fibroblastic spindle shape with eccentric irregular nuclei. The cells expressed surface markers viz., CD 105 and CD 106 besides expressing genes of collagen type II and I. Haematopoetic markers (CD 34 and CD 45) and aggrecan gene however, were not expressed. The rMSCs showed a moderate alkaline phosphates activity. Trilineage differentiation was conducted utilizing prepared differentiation media and rMSCs were differentiated into corresponding cell lineages based upon the medium used. It was concluded that rMSCs possess morphology similar to other species with good proliferation rate and exhibit the characteristics laid down by International Society for Cellular Therapy (ISCT). The present study provided basic protocols for characterization of rabbit MSCs that should be used before application of these cells for any research or therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

Gugjoo¹,

Amarpal²,

Kinjavdeka³

et al. 2015

Asian J. of Animal and Veterinary Advances

View full text Add to dashboard Cite

show abstract

“…These small rodents have maintenance costs that are ten times lower than those for rabbits. They also have short pregnancies, rapid growth and multiple offspring, as well as behavior that tolerate human handling [9]. In terms of equivalence, rats show similar limb anatomy and their genome is 80% to 90% homologous to that of humans [10] [11].…”

Section: Introductionmentioning

confidence: 99%

Complete Achilles Tenotomy: A New Improved Experimental Surgical Technique in Rats

Barbato¹,

Almeida²,

Costa³

et al. 2018

OJAS

View full text Add to dashboard Cite

Rupture of the Achilles tendon is one of the most frequent tendon injuries in humans. However, there is no standard surgical technique thus far to reproduce complete rupture of this tendon in rats. Surgical procedures were performed in Wistar rats under light microscopy, with posterior longitudinal incision and preservation of the plantaris tendon. The animals were sacrificed on 2 nd and 8 th postoperative week and we used hematoxilin and eosin, picrosirius-red and Weigert's resorcin-fuchsin to observe general tendon healing. The mean operative time was one minute and 24 seconds. Histological studies showed cellular proliferation and fibrillogenesis at two weeks, with decreased amounts of cellularity and elastic fibers at the 8 th week, besides changes in structural organization of collagen fibers. The complete Achilles tenotomy in rats, was improved under light microscopy and sparing the plantaris tendon. This surgical technique was simple and quick, showed signs of normal healing process, could be easily reproducible, and triggered translational researches in this area.

show abstract

“…Os ratos são considerados excelentes modelos experimentais para a espécie humana, devido a sua semelhança anatômica e fisiológica. Como exemplo são destacados os tendões 28 . Essa similaridade possibilita que as informações obtidas em pesquisas envolvendo diferentes tecidos e órgãos, possam ser utilizadas para o tratamento de afecções que afetam o homem.…”

Section: Introductionunclassified

Laserterapia Em Afecções Locomotoras: Revisão Sistemática De Estudos Experimentais

Souza

Silva

2016

Rev Bras Med Esporte

View full text Add to dashboard Cite

RESUMOIntrodução: Ao longo dos anos a fisioterapia tem contribuído para o tratamento das lesões musculotendí-neas e osteoarticulares pela utilização de diversos recursos eletrotermofototerapêuticos como, por exemplo, o laser de baixa potência. Objetivo: A finalidade desse estudo foi identificar mediante revisão sistemática, o efeito da laserterapia de baixa potência em afecções do sistema locomotor de ratos, com destaque para a dose de energia e o comprimento de onda utilizado, assim como para a resposta ao tratamento. Métodos: Foram consultadas as bases de dados Medline, Lilacs, PEDro e SciELO, entre janeiro de 2005 a maio de 2013, utilizando os termos "tendinopathy", "laser therapy", "rats", "tendon", "muscle", "bone", "low-level laser therapy", assim como "tendinopatia", "laserterapia", "ratos", "tendão", "músculo", "osso" e "laser de baixa potência". Apenas foram incluídos estudos experimentais publicados nos idiomas português e inglês, que induziram lesão em tendões, músculos, ossos e/ou articulação, tratada com laserterapia, associada ou não a outros tratamentos. Resultados: Foi encontrado um total de 30 estudos experimentais nas bases de dados consultadas, dos quais 15 (50%) foram realizados em tendão, 10 (33,33%) em músculo e cinco (16,66%) em osso e/ou articulação. As doses de energia mais comumente utilizadas foram de 3 J (26,66%) e 1 J (16,66%). Já os comprimentos de onda foram de 904 nm (21,21%) e 830 nm (21,21%). Conclusão: A informação mais expressiva obtida com a terapia foi a diminuição da resposta inflamatória (36,66%) em lesões ortopédicas agudas. May 2013, using the terms "tendinopathy", "laser therapy", "rats", "tendon", "muscle", "bone", "low-level laser therapy", as well as "tendinopatia", "laserterapia", "ratos", "tendão", " músculo", "osso" and "laser de baixa potência". Only experimental studies published in Portuguese and English and that induced lesion in tendons, muscles, bones and/or joints, treated Descritores

show abstract

Animal models for the study of tendinopathy

Cited by 132 publications

References 97 publications

Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

Isolation, Culture and Characterization of New Zealand White Rabbit Mesenchymal Stem Cells Derived from Bone Marrow

Complete Achilles Tenotomy: A New Improved Experimental Surgical Technique in Rats

Laserterapia Em Afecções Locomotoras: Revisão Sistemática De Estudos Experimentais

Contact Info

Product

Resources

About