Animal Test Models for Implant-Associated Inflammation and Infections

Rais, Bushra; Rahim, Muhammad Imran; Lienenklaus, Stefan; Tolle, Christian; Seitz, Jan‐Marten; Menzel, Henning; Hauser, Hansjörg; Müller, Peter

doi:10.1007/978-3-319-10981-7_12

Cited by 1 publication

(2 citation statements)

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“…Several fluorescent probes are commercially available that can be used to image inflammatory molecules . However, the resulting signals appear dispersed and cannot be used to distinguish multiple clustered implants in the same animal . Moreover, the light absorption and the fluorescence of ordinary tissue limits the detection sensitivity …”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the inflammatory potential of implant materials in a mouse model by bioluminescent imaging of intravenously injected bone marrow cells

Rais

Köster

Rahim

et al. 2016

J Biomedical Materials Res

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CitationEvaluation of the inflammatory potential of implant materials in a mouse model by bioluminescent imaging of intravenously injected bone marrow cells. 2016, 104 AbstractTo evaluate the inflammatory potential of implants a bioluminescent imaging assay was developed using luciferase-expressing bone marrow cells that were injected into the blood circulation of wild-type mice. After subcutaneous implantation of titanium discs as an example for a clinically established biocompatible material, the luminosity was modest.Similarly, low luminosity signals were generated by pure magnesium implants that were used to represent metallic alloys that are presently under investigation as novel degradable implant materials. Increased luminosity was observed in response to degradable polymeric PLGA implants. Surgical wounds induced a basic luminescent response even in the absence of an implant. However, the material-independent response to injury could be minimized using injectable microparticle suspensions. In parallel with the resorption of biodegradable 1 microparticles, the signal induced by PLGA declined faster when compared to the stable polymer polystyrene. By using an interferon type I inducible Mx2 promoter construct to drive luciferase gene expression, the highest luminosity was observed in response to bacteria, indicating that the system could also be employed to monitor implant infections. Overall, labeled bone marrow cells yielded specific, well-defined localized signals that correlated with the inflammatory responses to implants.

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Section: Introductionmentioning

confidence: 99%

“…25 However, the resulting signals appear dispersed and cannot be used to distinguish multiple clustered implants in the same animal. 26 Moreover, the light absorption and the fluorescence of ordinary tissue limits the detection sensitivity. 27 Bioluminescence could be used to evaluate the inflammatory potential of implant materials.…”

Section: Introductionmentioning

confidence: 99%