Anion- and Cation-Exchange MicroHPLC Utilizing Poly(methacrylates)-coated Monolithic Silica Capillary Columns

Ikegami, Tohru; Ichimaru, Jun; Kajiwara, Wataru; Nagasawa, Nozomi; Hosoya, Ken; Tanaka, Nobuo

doi:10.2116/analsci.23.109

Cited by 26 publications

(6 citation statements)

References 24 publications

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“…Monolithic silica capillary columns modified with 3-methacryloxypropyltriethoxysilane can be used as a universal platform for preparation of ion-exchange columns for HPLC of nucleic acids, nucleotides, and inorganic anions with separation efficiencies comparable with those of particle-packed columns [185].…”

Section: Ion-exchange and Ion Chromatographymentioning

confidence: 99%

Polymethacrylate monolithic columns for capillary liquid chromatography

Urban

Jandera²

2008

J of Separation Science

121

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In recent years, continuous separation media have attracted considerable attention because of the advantages they offer over packed columns. This research resulted in two useful monolithic material types, the first based on modified silica gel and the second on organic polymers. This work attempts to review advances in the development, characterization, and applications of monolithic columns based on synthetic polymers in capillary chromatography, with the main focus on monolithic beds prepared from methacrylate-ester based monomers. The polymerization conditions used in the production of polymethacrylate monolithic capillary columns are surveyed, with attention being paid to the concentrations of monomers, porogen solvents, and polymerization initiators as the system variables used to control the porous and hydrodynamic properties of the monolithic media. The simplicity of their preparation as well as the possibilities of controlling of their porous properties and surface chemistries are the main benefits of the polymer monolithic capillary columns in comparison to capillary columns packed with particulate materials. The application areas considered in this review concern mainly separations in reversed-phase chromatography, ion-exchange chromatography, hydrophobic and hydrophilic interaction modes; enzyme immobilization and sample preparation in the capillary chromatography format are also addressed.

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Section: Ion-exchange and Ion Chromatographymentioning

confidence: 99%

Polymethacrylate monolithic columns for capillary liquid chromatography

Urban

Jandera²

2008

J of Separation Science

121

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“…In a similar manner, weak cation-exchange ( 85) and strong cation-exchange (SCX) columns can also be prepared using methacrylates possessing carboxylic acids and sulfonic acids (91). Readers should keep in mind that IEX columns can be used as HILIC columns.…”

Section: Hydrophilic Interaction Liquid Chromatographymentioning

confidence: 99%

Recent Progress in Monolithic Silica Columns for High-Speed and High-Selectivity Separations

Ikegami

Tanaka²

2016

Annual Rev. Anal. Chem.

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Monolithic silica columns have greater (through-pore size)/(skeleton size) ratios than particulate columns and fixed support structures in a column for chemical modification, resulting in high-efficiency columns and stationary phases. This review looks at how the size range of monolithic silica columns has been expanded, how high-efficiency monolithic silica columns have been realized, and how various methods of silica surface functionalization, leading to selective stationary phases, have been developed on monolithic silica supports, and provides information on the current status of these columns. Also discussed are the practical aspects of monolithic silica columns, including how their versatility can be improved by the preparation of small-sized structural features (sub-micron) and columns (1 mm ID or smaller) and by optimizing reaction conditions for in situ chemical modification with various restrictions, with an emphasis on recent research results for both topics.

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“…In many cases, this is paired with the necessity of anhydrous reaction conditions. Macromolecules can for example be coupled to HPLC silica packing materials through direct silylation procedures [1,2], epoxide ring opening processes of 3-glycidoxypropyltrimethoxysilane derivatised silica [3] or by amidation or reductive amination on aminopropyl-silica, respectively [4][5][6]. Synthetic polymers are also sometimes polymerized on the supporting material via grafting from types of approaches [7].…”

Section: Introductionmentioning

confidence: 99%