Anion-exchange chromatography using short monolithic columns as a complementary technique for human serum albumin depletion prior to human plasma proteome analysis

Petrič, Tatjana Čerk; Brne, Peter; Gabor, Boštjan; Govednik, Lidija; Barut, Miloš; Štrancar, Aleš; Kralj, Lucija Zupančič

doi:10.1016/j.jpba.2006.06.019

Cited by 32 publications

(20 citation statements)

References 11 publications

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“…One of the simple ones was a short monolithic column utilizing anionexchange chromatography [70]. Multistep processes include the above described salting out method (section 2.1) to first precipitate the immunoglobulins from human plasma (4 M ammonium sulfate) followed by fractionating with anion exchange chromatography using diethylaminoethyl (DEAE)-cellulose column to acquire pure IgG [71].…”

Section: Non-affinity-based Depletion Methodsmentioning

confidence: 99%

Medicinal Chemistry Meets Proteomics: Fractionation of the Human Plasma Proteome

Kovács¹,

Guttman²

2013

CMC

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Human plasma and its fractions/derivatives are frequently used materials in biomedicine as it contains thousands and thousands of proteins representing the majority of human proteome. Several important methods were developed in the past for the fractionation of this important biological fluid and their use for medicinal purposes. One of the greatest challenges is the very large dynamic range of plasma proteins ranging up to 10-12 orders of magnitude. Early attempts were mainly based on methods such as salting out or cold ethanol precipitation, as well as chromatography utilizing affinity, size exclusion, ion exchange and hydrophobic interaction techniques. More recently, fractionation applications started with the depletion of the high abundant plasma components, such as serum albumin and immunoglobulins, before isolating lower abundant proteins of interest. Plasma volumes were utilized from the milliliter scale for diagnostic applications to hundreds of liters for industrial scale plasma fractionation (e.g., medicinal product manufacturing). In this paper we review this important part of medicinal chemistry, highlighting the traditional methods along with some of their variations as well as the most significant recent achievements of the field.

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Section: Non-affinity-based Depletion Methodsmentioning

confidence: 99%

Medicinal Chemistry Meets Proteomics: Fractionation of the Human Plasma Proteome

Kovács¹,

Guttman²

2013

CMC

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“…Nitrogen adsorption experiments were performed and the specific surface area (51.16 m 2 •g -1 ) and pore volume (0.14 cm 3 •g -1 ) of monolithic polymer were obtained. The average pore diameter was calculated to be 14.3 nm (BJH method).…”

Section: Morphological Characteristic Of Monolithic Columnmentioning

confidence: 99%

“…[3][4][5][6][7] The detection of drugs in biological samples normally involves a sample treatment step before injection into the HPLC system. Typically, the extraction of drugs is performed using protein precipitation by organic solvents, [8][9][10] liq- uid-liquid extraction 11,12 and off-line SPE.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Caffeine and Theophylline in Human Plasma with a Weak Cation Monolithic SPE‐column

Zhu

Row²

2010

Chin. J. Chem.

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A simple reversed-phase high performance liquid chromatography (RP-HPLC) method was developed to determine the level of caffeine and theophylline in human plasma samples. The sample clean-up step involved the on-line solid-phase extraction (SPE) of the analytes from plasma samples into a weak cation monolithic column using a column switching system. Separation was performed on a C 18 column (5 µm, 150 mm×4.6 mm) with ultraviolet detection at 274 nm. The mobile phase consisted of methanol-water (32/68, V/V) under isocratic conditions at a flow rate of 0.6 mL•min -1 . The measured concentration of caffeine and theophylline showed a good linear relationship over the concentrations range, 0.1-80.0 µg•mL -1 . The absolute recoveries ranged from 77.10% to 85.39%, and the inter-day and intra-day relative standard deviations (RSD) were all less than 5%. This method avoids a tedious pretreatment and provides an economic, repeatable and effective method for assaying trace drugs in biological samples.

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“…Several researchers have demonstrated that depletion of most abundant plasma proteins amplifies the contribution of least abundant plasma proteins, thus creating the opportunity to uncover and study specific proteins/peptides. 5,6 Polymethacrylate chromatographic monoliths afford flow rate independent binding capacity and resolution for large biomolecules due to the convective nature of the flow, which allows relatively short analysis times compared to traditional chromatographic supports. 7 Having these advantages in mind we decided to partially deplete human serum albumin from plasma sample using chromatographic monoliths bearing immunoaffinity ligand.…”

Section: Introductionmentioning

confidence: 99%

Use of Differential Scanning Calorimetry and Immunoaffinity Chromatography to Identify Disease Induced Changes in Human Blood Plasma Proteome

Brudar

Černigoj

Podgornik

et al. 2017

ACSi

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Differential scanning calorimetry provides unique signatures of blood plasma samples. Plasma samples from diseased individuals yield specific thermograms, which differ from each other and from plasma samples of healthy individuals. Thermograms from individuals suffering from chronic lymphocytic leukemia, multiple myeloma and acute myeloid leukemia were measured with DSC. To obtain additional information about thermal behaviour of plasma proteins immunoaffinity chromatography was introduced. An immunoextraction of HSA using a chromatographic column with immobilized anti-HSA was carried out in order to enrich less abundant plasma proteins, which could provide a further insight into disease development. Efficiency of HSA depletion and protein composition of fractionated plasma was validated by SDS-PAGE.

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Anion-exchange chromatography using short monolithic columns as a complementary technique for human serum albumin depletion prior to human plasma proteome analysis

Cited by 32 publications

References 11 publications

Medicinal Chemistry Meets Proteomics: Fractionation of the Human Plasma Proteome

Medicinal Chemistry Meets Proteomics: Fractionation of the Human Plasma Proteome

Simultaneous Determination of Caffeine and Theophylline in Human Plasma with a Weak Cation Monolithic SPE‐column

Use of Differential Scanning Calorimetry and Immunoaffinity Chromatography to Identify Disease Induced Changes in Human Blood Plasma Proteome

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