Anisotropic conductivity for single-cell electroporation simulation with tangentially dispersive membrane

Guo, Fei; Qian, Kun; Zhang, Lin; Deng, Hao; Li, Xin; Zhou, Jiong; Wang, Ji

doi:10.1016/j.electacta.2021.138426

Cited by 11 publications

(5 citation statements)

References 36 publications

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“…The maximum membrane conductivity for σ Low , σ Med , and σ High buffers are 0.01 mS/m, 0.2 mS/m, and 0.5 mS/m, respectively, as shown in Figure 2. Even though we did not replicate the recent electroporated membrane conductivity of 3 mS/m to 30 mS/m [37][38][39], our results are consistent with some early experimental and theoretical works (0.01 mS/m to 1 mS/m) [32,[40][41][42]. Ramos et al [43] shows a membrane conductivity of 1 µS/m to 0.01 mS/m (conversion σ m = h•G m [44]) for yeast cells with 0.28 volumetric fraction.…”

Section: Discussioncontrasting

confidence: 80%

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Pintarelli

Silva

Yang

et al. 2022

Sensors

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A specific pulsed electric field protocol can be used to induce electroporation. This is used in the food industry for yeast pasteurization, in laboratories for generic transfer and the medical field for cancer treatment. The sensing of electroporation can be done with simple ‘instantaneous’ voltage-current analysis. However, there are some intrinsic low-frequency phenomena superposing the electroporation current, such as electrode polarization. The biological media are non-homogeneous, giving them specific characterization in the broad frequency spectrum. For example, the cell barrier, i.e., cell membrane, causes so called β-dispersion in the frequency range of tens to thousands of kHz. Electroporation is a dynamic phenomenon characterized by altering the cell membrane permeability. In this work, we show that the impedance measurement at certain frequencies could be used to detect the occurrence of electroporation, i.e., dielectric dispersion modulated sensing. This approach may be used for the design and implementation of electroporation systems. Yeast suspension electroporation is simulated to show changes in the frequency spectrum. Moreover, the alteration depends on characteristics of the system. Three types of external buffers and their characteristics are evaluated.

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Section: Discussioncontrasting

confidence: 80%

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Pintarelli

Silva

Yang

et al. 2022

Sensors

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“…During the rise-and fall-time of the current pulse, the absolute value of TMP exceeded 1 V, and the obvious changes in the pore density were observed. After a two-step increment, the pore density reached 1.5 × 10 10 m −2 at this point, though did exceed the the conventional electroporation criterion 10 14 m −2 [41,42].…”

Section: Sub-microsecond and Nanosecond Pulse Simulation Resultsmentioning

confidence: 85%

Simulation study of cell transmembrane potential and electroporation induced by time-varying magnetic fields

Guo

Qian

et al. 2022

Innovative Food Science & Emerging Technologies

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“…Electroporation (EP) is a technique where electrical pulses are used in forming temporary pores in the plasma membrane to penetrate the chemotherapeutic agents into the tumor cells. [17] There have been many studies on EP in the last few decades, and it has found application in several fields like medicine, biochemistry, biology, and biotechnology. It involves the intratumor or intra-venous application of the anti-cancer agents and then the administration of electric fields on the tumor area.…”

Section: Introductionmentioning

confidence: 99%

Electroporation Enhances the Anticancer Effects of Novel Cu(II) and Fe(II) Complexes in Chemotherapy‐Resistant Glioblastoma Cancer Cells

Alkış

Buldurun

Alan

et al. 2023

Chemistry & Biodiversity

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Schiff base ligand (L) was obtained by condensation reaction between 4-aminopyrimidin-2(1H)-one (cytosine) with 2-hydroxybenzaldehyde. The synthesized Schiff base was used for complexation with Cu(II) and Fe(II) ions used by a molar (2 : 1 mmol ration) in methanol solvent. The structural features of ligand, Cu(II), and Fe(II) metal complexes were determined by standard spectroscopic methods (FT-IR, elemental analysis, proton and carbon NMR spectra, UV/VIS, and mass spectroscopy, magnetic susceptibility, thermal analysis, and powder X-ray diffraction). The synthesized compounds (Schiff base and its metal complexes) were screened in terms of their anti-proliferative activities in U118 and T98G human glioblastoma cell lines alone or in combination with electroporation (EP). Moreover, the human HDF (human dermal fibroblast) cell lines was used to check the biocompatibility of the compounds. Anti-proliferative activities of all compounds were ascertained using an MTT assay. The complexes exhibited a good anti-proliferative effect on U118 and T98G glioblastoma cell lines. In addition, these compounds had a negligible cytotoxic effect on the fibroblast HDF cell lines. The use of compounds in combination with EP significantly decreased the IC 50 values compared to the use of compounds alone (p < 0.05). These results show that newly synthesized Cu(II) and Fe(II) complexes can be developed for use in the treatment of chemotherapy-resistant U118 and T98G glioblastoma cells and that treatment with lower doses can be provided when used in combination with EP.

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Anisotropic conductivity for single-cell electroporation simulation with tangentially dispersive membrane

Cited by 11 publications

References 36 publications

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Simulation study of cell transmembrane potential and electroporation induced by time-varying magnetic fields

Electroporation Enhances the Anticancer Effects of Novel Cu(II) and Fe(II) Complexes in Chemotherapy‐Resistant Glioblastoma Cancer Cells

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