Annexin A2 as a target endothelial cell membrane autoantigen in Behçet's disease

Chen, Peng-Peng; Yan, Haidun; Tian, Yaping; Xun, Yiping; Shi, Lili; Bao, Rong; Zhang, Huailing; Chen, Guangyu; Yang, Chunhe; Sun, Shutao; Wang, Yajie; Liu, Li; Zhou, Yabin; Zhang, Chunyan; Wang, Xiaoxu; Wen, Yongqiang; Bian, Yongzhong; Du, Hongwu

doi:10.1038/srep08162

Cited by 24 publications

(14 citation statements)

References 52 publications

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“…In our previous studies, immunological analyses were used to make a contribution to the identification of potential diagnostic biomarkers specific to immune-mediated diseases and other vasculitis-associated diseases [11]. In our previous studies, immunological analyses were used to make a contribution to the identification of potential diagnostic biomarkers specific to immune-mediated diseases and other vasculitis-associated diseases [11].…”

Section: Introductionmentioning

confidence: 99%

“…Comprehensive utilization of bioinformatics, immunology and molecular biology methods to screen and identify potential biomarkers of disease efficiently is a universally recognized disease target discovery strategy. In our previous studies, immunological analyses were used to make a contribution to the identification of potential diagnostic biomarkers specific to immune-mediated diseases and other vasculitis-associated diseases [11]. As is widely known in the early diagnosis of many vasculitis and autoimmune diseases, there are some specific immune attack targets, and the related detection of antibody levels in the serum of patients has important reference value in the diagnosis of diseases [12].…”

Section: Introductionmentioning

confidence: 99%

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PGK1 as an immune target in Kawasaki disease

Cui

Zhou

et al. 2018

Clinical and Experimental Immunology

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Kawasaki disease (KD) is an immune-mediated vasculitis with symptoms that mimic febrile illness; the immune origin has been suggested but never proved. First, cell chip technology was used to screen immune targets cells. With the indirect immunofluorescence assay we found that the HeLa cell chip could be recognized by KD patient serum samples. The target cell proteome was extracted as antigens, and antigen recognition reaction was performed using the patients' serum as antibodies and the detected target protein was detected and identified as phosphoglycerate kinase 1 (PGK1). Then PGK1 was produced and tested with enzyme-linked immunosorbent assay (ELISA), Western blotting, immunoprecipitation and competitive inhibition immunofluorescence assay. Immunoglobulin (Ig)G against PGK1 was detected in 46% (23 of 50) sera of KD patients, 13% (five of 38) sera in febrile non-KD patients (FC) and 2·6% (one of 38) sera in healthy donors. As an immune target, PGK1 not only helps understanding of the pathogenesis, it also has potential value in facilitating the laboratory diagnosis of KD.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PGK1 as an immune target in Kawasaki disease

Cui

Zhou

et al. 2018

Clinical and Experimental Immunology

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“…Recently, at least four new targets of AECA in BD, i.e. prohibitin, HSP27, ETFB and annexin A2, have been successfully screened out by our group [5][6][7][8][9]. These findings not only added knowledge to explain the AECA related pathology, but also further confirmed the key role of AECA in the process of BD.…”

Section: Introductionmentioning

confidence: 58%

Moesin expression is correlated with its involvement in patients with Behcet’s disease

Hussain

Chen

Zhang

et al. 2020

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Introduction: Behcet's disease (BD) is a rare, chronic autoimmune disorder of unknown etiology. Although the profile of autoantibodies for this disease is not yet completely understood, because of better disease recognition, its prevalence is increasing throughout the world. Among ERM proteins (ezrin/radixin/moesin), moesin is a member of a family which is involved in autoimmune diseases. The aim of this study is to confirm whether moesin is a potential anti-endothelial cell autoantigen (AECA) in Hans Chinese BD patients. Material and methods: First, a full length recombinant human moesin protein was over-expressed and purified. Second, it was identified by mass spectrometry and then purified moesin was used to perform Western blotting, immunoprecipitation and ELISA with confirmed BD patients. Finally, in vitro cytotoxicity experiments were conducted with anti-moesin antibodies by the resazurin reduction assay method. Results: Purified moesin protein was successfully expressed and then its antigenicity was confirmed by Western blotting and immunoprecipitation techniques. Anti-moesin antibodies were detected in approximately onethird (38%) of BD patients by ELISA and the reactivity of BD serum IgG antibodies against moesin was found to be significantly higher than HC (p < 0.0001). Moreover, in order to validate our results, cytotoxicity experiments also confirmed that anti-moesin antibody had a significant inhibitory effect on endothelial cell activity. Conclusions: Expression is correlated with the involvement of moesin as an autoantigen in BD pathology, which is a new finding. It might be a new candidate biomarker in the Han Chinese population.

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“…Self-antibodies which can activate the endothelial cells and the inflammatory response have been found to play an important role in the pathological process of BD [4]. Recently, four target antigens of AECA in BD -prohibitin, ETFB, HSP27 and annexin A2 -have been successfully identified in our laboratory [5][6][7][8]. However, more potential immune targets should be found to understand the exact pathogenesis of BD.…”

Section: Introductionmentioning

confidence: 99%

Anti-lamin A/C antibodies in patients with Behçet’s disease

Chen¹,

Yan²,

Zhang³

et al. 2018

cejoi

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Lamin a/C has been identified as a target antigen of anti-endothelial cell antibodies in vasculitis. Behçet's disease (BD) is a chronic vasculitis with unclear pathogenesis. Self-antibodies have been found to play an important role in the pathological process of BD. The aim of this study was to verify whether lamin A/C is an autoantigen of BD. First, the human lamin A/C protein was cloned and purified and verified by Western blotting. Next, a homemade ELISA kit was used to test the presence of anti-lamin A/C antibodies in BD patients. This is the first time it has been certified that lamin A/C is an autoantigen of BD. The reactivity of serum IgG against recombinant human lamin A/C was detected in 7 of 31 BD patients (23%) and 3 of 32 healthy controls (HC) (9%).

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Annexin A2 as a target endothelial cell membrane autoantigen in Behçet's disease

Cited by 24 publications

References 52 publications

PGK1 as an immune target in Kawasaki disease

PGK1 as an immune target in Kawasaki disease

Moesin expression is correlated with its involvement in patients with Behcet’s disease

Anti-lamin A/C antibodies in patients with Behçet’s disease

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