Annexin V and platelet antigen expression is not altered during storage of platelet concentrates obtained with the AMICUS™ cell separator

Gutensohn, K.; Alisch, A.; Geidel, K.; Crespeigne, N.; Kuehnl, P.

doi:10.1016/s0955-3886(99)00021-1

Cited by 6 publications

(1 citation statement)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It mediates the binding of activated platelets to neutrophils, monocytes, and endothelial cells (3). P-selectin expression on platelet surfaces correlates with loss of viability upon transfusion of the stored platelet concentrates (4).…”

Section: Introductionmentioning

confidence: 99%

Platelet Function, Activation and Apoptosis During and After Apheresis

et al. 2010

View full text Add to dashboard Cite

Platelets are known to undergo shape change, activation, release reaction and apoptosis/necrosis during processing and storage. Apheresis may have a deleterious impact on platelet achievability and functional integrity. Platelet concentrates from 50 male volunteers obtained by COBE spectra were screened for platelet activation (CD62 and CD154) and apoptosis (phosphatidylserine detected by Annexin V). Donor samples before separation, during apheresis and at the third day of storage were used as baseline donor samples. Platelet aggregation to adenosine diphosphate (ADP) and collagen was performed. There was a statistically significant increase in the expression of activation markers in two different samples (during separation samples and third day samples). Although the increase in Annexin V expression was not so observable, it showed a significant increase also. There was marked decline in the platelet aggregation. The correlations between the values of CD62, CD154 and Annexin V were detected in baseline samples and increased during separation and at the third day of platelets storage. Correlation between values of platelet aggregation to collagen and Annexin V was relevant only in the baseline samples. No other correlations were encountered between platelet aggregation and markers of activation and apoptosis during apheresis and storage. Initial platelet activation induced by apheresis may have an impact on phosphatidylserine expression with no impact on aggregation function of platelets during storage.

show abstract

Section: Introductionmentioning

confidence: 99%