Anoectochilus roxburghii : A review of its phytochemistry, pharmacology, and clinical applications

Ye, Shenyi; Shao, Qingsong; Zhang, Ailian

doi:10.1016/j.jep.2017.07.032

Cited by 103 publications

(68 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Due to the fact of its high medical values, A. roxburghii is not only widely used in medicine and health care but also in beauty and drinking products with an increasing market demand. The average annual demand in South Korea and Japan is more than 1000 t, 70% of which depend on imports [10]. The vigorous market demand results in excessive harvesting and exploitation of the plant, leading to resource depletion.…”

Section: Introductionmentioning

confidence: 99%

“…MF23 could also stimulate the accumulation of flavonoid and kinsenoside in A. formosanus [16]. For A. roxburghii, the major bioactive components include polysaccharides, kinsenosides, steroids, triterpenes, amino acids, alkaloids, and flavonoids that have been regarded as the quality standard of A. roxburghii [10]. However, A. roxburghii contains a very limited number of flavonoids, which limits the development and utilization of A. roxburghii-based medicines.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Combined Metabolome and Transcriptome Analyses Reveal the Effects of Mycorrhizal Fungus Ceratobasidium sp. AR2 on the Flavonoid Accumulation in Anoectochilus roxburghii during Different Growth Stages

Zhang

Chen

et al. 2020

IJMS

View full text Add to dashboard Cite

Anoectochilus roxburghii is a traditional Chinese herb with high medicinal value, with main bioactive constituents which are flavonoids. It commonly associates with mycorrhizal fungi for its growth and development. Moreover, mycorrhizal fungi can induce changes in the internal metabolism of host plants. However, its role in the flavonoid accumulation in A. roxburghii at different growth stages is not well studied. In this study, combined metabolome and transcriptome analyses were performed to investigate the metabolic and transcriptional profiling in mycorrhizal A. roxburghii (M) and non-mycorrhizal A. roxburghii (NM) growth for six months. An association analysis revealed that flavonoid biosynthetic pathway presented significant differences between the M and NM. Additionally, the structural genes related to flavonoid synthesis and different flavonoid metabolites in both groups over a period of six months were validated using quantitative real-time polymerase chain reaction (qRT-PCR) and high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). The results showed that Ceratobasidium sp. AR2 could increase the accumulation of five flavonol-glycosides (i.e., narcissin, rutin, isorhamnetin-3-O-beta-d-glucoside, quercetin-7-O-glucoside, and kaempferol-3-O-glucoside), two flavonols (i.e., quercetin and isorhamnetin), and two flavones (i.e., nobiletin and tangeretin) to some degrees. The qRT-PCR showed that the flavonoid biosynthetic genes (PAL, 4CL, CHS, GT, and RT) were significantly differentially expressed between the M and NM. Overall, our findings indicate that AR2 induces flavonoid metabolism in A. roxburghii during different growth stages, especially in the third month. This shows great potential of Ceratobasidium sp. AR2 for the quality improvement of A. roxburghii.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combined Metabolome and Transcriptome Analyses Reveal the Effects of Mycorrhizal Fungus Ceratobasidium sp. AR2 on the Flavonoid Accumulation in Anoectochilus roxburghii during Different Growth Stages

Zhang

Chen

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Anoectochilus roxburghii (hereafter shortened as A. roxburghii), a traditional herb used in China for medicinal and culinary purposes, grows in the wet place under the evergreen broadleaved or bamboo forest, which contains several bioactive compounds, such as kinsenoside, polysaccharides, flavonoids, and glycosides [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

“…Among those, kinsenoside (3-O-β-D-glucopyranosyl-(3R)-hydroxy butanolide) is a major compound of A. roxburghii [1,3]. Ito et al [4] first reported the isolation and structural characterization of it, which exists in Anoectochilus koshunensis and Anoectochilus formosanus.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM)

et al. 2020

View full text Add to dashboard Cite

The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant Anoectochilus roxburghii. Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenoside, while response surface method (RSM) was used to optimize the extraction process. The best possible range for methanol concentration (0–100%), the liquid-solid ratio (5:1–30:1 mL/g), ultrasonic power (240–540 W), duration of ultrasound (10–50 min), ultrasonic temperature (10–60 °C), and the number of extractions (1–4) were obtained according to the single factor experiments. Then, using the Box-Behnken design (BBD) of response surface analysis, the optimum extraction conditions were obtained with 16.33% methanol concentration, the liquid-solid ratio of 10.83:1 mL/g and 35.00 °C ultrasonic temperature. Under these conditions, kinsenoside extraction yield reached 32.24% dry weight. The best conditions were applied to determine the kinsenoside content in seven different cultivation ages in Anoectochilus roxburghii.

show abstract

“…formosanus has high medicinal value, the main chemical components in A. formosanus are polysaccharides, flavonoids, glycosides, organic acids, steroids, trienes, alkaloids and nucleosides. These compounds have been shown to be the main biologically active substances, with major pharmacological effects such as anti-diabetes, anti-inflammatory, antiinflammatory, anti-viral, liver protection, kidney protection, immunomodulation and anti-tumor [4]. A. formosanus is considered to be a precious medicinal material in China.…”

Section: Introductionmentioning

confidence: 99%

Induction of Adventitious buds from stem explants inAoectochilus formosanus

Xue

Xiao

et al. 2019

E3S Web Conf.

View full text Add to dashboard Cite

In order to explore the optimal culture conditions for adventitious bud regeneration of stem explants in Aoectochilus formosanus, the stem segments from the sterile seedlings were used as explants, and different concentrations of diethyl aminoethyl hexanoate (DA-6), kinetin (KT), Cu2+ and glutamine (Gln) were separately added into MS medium containing 6-benzylaminopurine (6-BA) and naphthaleneacetic acid (NAA), and the induction rate and the induction multiple of adventitious buds were recorded and analyzed. The results showed that the regeneration of adventitious buds could be promoted, when 2 mg/L DA-6, 0.4 mg/L KT and 15 mg/L Gln were added in mediums. However, the effect of Cu2+ on the regeneration of adventitious buds in A. formosanus was enhanced by low concentration and suppressed by high concentration, and the best concentration of Cu2+ was 5 mg/L.

show abstract

Anoectochilus roxburghii : A review of its phytochemistry, pharmacology, and clinical applications

Cited by 103 publications

References 21 publications

Combined Metabolome and Transcriptome Analyses Reveal the Effects of Mycorrhizal Fungus Ceratobasidium sp. AR2 on the Flavonoid Accumulation in Anoectochilus roxburghii during Different Growth Stages

Combined Metabolome and Transcriptome Analyses Reveal the Effects of Mycorrhizal Fungus Ceratobasidium sp. AR2 on the Flavonoid Accumulation in Anoectochilus roxburghii during Different Growth Stages

Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM)

Induction of Adventitious buds from stem explants inAoectochilus formosanus

Contact Info

Product

Resources

About