Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus

Gaínza, Yousmel Alemán; Domingues, Luciana Ferreira; Pérez, Oriela Pino; Rabelo, M. D.; López, Eugenio Roque; Chagas, A. C. de S.

doi:10.1016/j.indcrop.2015.07.056

Cited by 45 publications

(20 citation statements)

References 44 publications

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“…Limonene is found in oils from different plants with anthelmintic activity, such as Lippia sidoides, Cymbopogon martinii, Mentha piperita and Eucalyptus staigeriana (Katiki et al, 2011;Carvalho et al, 2012;Ribeiro et al, 2013). In agreement with this finding, it has been found that limonene accounts for 96% of Citrus sinensis essential oil composition, causing 100% in vitro inhibition of H. contortus eggs and larvae in most concentrations tested (Gaínza et al, 2015). It is possible that these two compounds present in A. graveolens essential oil may also be the major components of the anthelmintic action on H. contortus observed in the present study.…”

Section: Discussionsupporting

confidence: 65%

Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

et al. 2021

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Gastrointestinal nematodes are responsible for great economic losses in sheep raising, and their control has long been carried out almost exclusively by the administration of anthelmintics, which have led to serious resistance problems. In the search for alternative control measures, phytotherapic research is highlighted. The aim of this study was to evaluate the action of Anethum graveolens (dill) essential oil on different stages of Haemonchus contortus life cycle, as well its cytotoxicity MDBK (Madin-Darby bovine kidney) cells. H. contortus larvae and eggs were obtained from infected sheep feces, and essential oil extracted from plant seeds through the Clevenger apparatus. 9.4, 4.7, 2.35, 1.17. 0.58 and 0.29 mg/mL concentrations were evaluated. The Egg Hatch Inhibition (HI), Larval Development Inhibition (LDI) and Larval Migration Inhibition (LMI) techniques were used. Thybendazole 0.025 mg/mL in HI and Levamisole 0.02 mg/mL in the LDI and LMI tests were used as positive controls, while distilled water and a Tween 80 solution were used as positive negative controls. The inhibition results obtained for the highest oil concentration were: HI 100%, LDI 98.58% and LMI 63.7%, differing ( <0.05) from negative controls. Main A. graveolens oil components present in 95.93% of the total oil were Dihydrocarvone (39.1%), Carvone (22.24%), D-Limonene (16.84%), Apiol (10.49%) and Trans-dihydrocarvone (7.26%). Minimum A. graveolens essential oil concentrations required to inhibit 50% (IC50) of egg hatching, larval development and larval migration were 0.006 mg/mL, 2.536 mg/mL and 3.963 mg/mL, respectively. Cell viability in MDBK (Madin-Darby bovine kidney) cells, when incubated with A. graveolens essential oil, was 86% for the highest (9.4 mg/mL) and 99% for the lowest concentration (0.29 mg/mL). A. graveolens essential oil, according to the results obtained in this study, is a promising alternative in sheep gastrointestinal nematode control. ResumoOs nematoides gastrintestinais são responsáveis por grandes perdas econômicas na ovinocultura, e seu controle tem sido realizado quase exclusivamente pela administração de anti-helmínticos, que levaram a sérios problemas de resistência. Na busca de medidas alternativas de controle, destaca-se a pesquisa fitoterápica. O objetivo deste trabalho foi avaliar a ação do óleo essencial de Anethum graveolens (endro) em diferentes estágios de Haemonchus contortus, bem como testar a viabilidade celular para o óleo. Larvas e ovos de H. contortus foram obtidos de fezes de ovinos infectados e óleo essencial extraído de sementes de plantas através do aparelho de Clevenger. As concentrações avaliadas foram 9,4, 4,7, 2,35, 1,17, 0,58 e 0,29 mg/mL. Verificou-se a Inibição de eclosão dos ovos (IE), Inibição de Desenvolvimento Larval (IDL) e Inibição de Migração Larval (IML). Tiabendazol 0,025 mg/mL em IE e Levamisole 0.02 mg/mL nos testes IDL e IML foram usados como controles positivos, enquanto água destilada e uma solução Tween 80 foram usados como controles negativos. Os resultados de inibição o...

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Section: Discussionsupporting

confidence: 65%

Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

et al. 2021

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“…), based on inhibition of egg hatching and larval development of H. contortus [58] . The sesquiterpene longifolene, a secondary metabolite, was shown to have ovicidal and larvicidal action on H. contortus, through the ability to mimic the juvenile hormone [59] . Lorimer et al [60] also identified the action of polyphenol compounds to inhibit the migration of third-stage nematode larvae.…”

Section: Compounds Of Plant Origin With Anthelmintic Actionmentioning

confidence: 99%

“…Eggs and larvae Inhibition of hatching and larval development [59] Artemisia annua Bunostomum sp., Cooperia…”

Section: H Contortusmentioning

confidence: 99%

Plants and their medicinal potential for controling gastrointestinal nematodes in ruminants

Borges¹,

Borges²

2016

Nematoda

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HIGHLIGHTS• Plants with anthelmintic activity may have potential sources of chemical substances that can be used to control gastrointestinal nematodes in ruminants.• The main difficulty of the research with herbal medicines is the lack of in vitro and in vivo correlation with phytotherapic substances activities.• Better understanding the interactions between metabolites and the use of appropriate methodologies to evaluate phytotherapics, may contribute to in vivo results. ABSTRACT:Gastrointestinal nematodes (GINs) is one of the main sanitary problems of ruminant herds, causing large economic losses to breeders. Chemical control continues to be the main measure used to combat GINs, but as resistant parasite populations spread, the efficiency of this method is becoming impaired. In light of this situation, alternative ways to control verminosis are gaining importance. Among these, phytotherapy has attracted particular attention. This review presents the current status of research on phytotherapeutic agents, their difficulties and perspectives. Plants with nematicidal activity are a potential source of chemical substances, including new molecules, that can be used to control GINs in ruminants. The principal difficulty of research into phytotherapic substances is to identify the bioactive compounds, because in many cases in vitro tests have shown promising results, but in vivo test results have been disappointing. Among the causes is host-drug interaction. Therefore, more careful choice of plants with anthelmintic potential by adoption of a standardized system to analyze the results of in vitro testing, combined with better understanding of the synergistic relations among metabolites and the adjustment of methods for in vivo testing, can make phytotherapy for control of GINs more effective.

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“…[1][2][3] Essential oils (EOs) are defined as a complex mixture of volatile substances, which can be extracted from different parts of the plant such as flowers, fruits, seeds, leaves, wood, bark and roots. 4 In addition, several biological activities have been attributed to EOs including antiviral, 5,6 insecticidal, 7 antiparasitic, 8,9 antimicrobial and antioxidant. [10][11][12] Dengue, Zika and Chikumgunya are arboviruses with the highest circulation in Brazil, they are characterized by a group of viral diseases, transmitted by vectors, which can be transmitted to humans and other animals through the bite of Aedes aegypti.…”

Section: Introductionmentioning

confidence: 99%

Circadian Rhythm and Larvicidal Activity Against Aedes aegypti of Essential Oils from Croton piauhiensis

Silva¹,

Silva²,

Vale³

et al. 2019

Rev. Virtual Quim.

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Ritmo Circadiano e Atividade Larvicida contra Aedes aegypti de Óleos Essenciais de Croton piauhiensis Resumo: Croton piauhiensis Müll. Arg., popularmente conhecido como velame, é uma espécie restrita do bioma Caatinga. Neste contexto, este trabalho tem como objetivo estudar pela primeira vez o ciclo circadiano e a atividade larvicida contra o Aedes aegypti dos óleos essenciais de C. piauhiensis. A composição química dos óleos essenciais de folhas obtidas por hidrodestilação foi analisada e identificada por GC-MS e GC-FID. De acordo com os resultados, o estudo circadiano mostrou diferenças na composição química dos óleos extraídos em diferentes horas do dia das folhas de C. piauhiensis. Os principais componentes dos óleos essenciais das folhas de C.

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Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus

Cited by 45 publications

References 44 publications

Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

Antihelminthic action of the Anethum graveolens essential oil on Haemonchus contortus eggs and larvae

Plants and their medicinal potential for controling gastrointestinal nematodes in ruminants

Circadian Rhythm and Larvicidal Activity Against Aedes aegypti of Essential Oils from Croton piauhiensis

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