Anthelmintic activity of a traditionally used root extract from Paullinia pinnata

Spiegler, Verena; Peppler, Carolin; Werne, Steffen; Heckendorn, Félix; Sendker, Jandirk; Liebau, Eva; Agyare, Christian; Hensel, Andreas

doi:10.1055/s-0036-1596924

Cited by 2 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our trials indicated a great variability of results depending on each particular polyphenol and concentration tested. Contrary to published studies on the AH effects of CT and HT plant rich-extracts against C. elegans (Katiki et al, 2013;Spiegler et al, 2016), purified monomers did not disclosed neither lethality nor "bagging effects" (hatching inside adult hermaphrodite worms) (Bull et al, 2007;Saul et al, 2010) on G1. However, some adults were missed from the agar surface not due to deceases.…”

Section: Discussioncontrasting

confidence: 97%

“…The general pattern for this monomer, seems to be associated with a net increase of fertility and percentages of hatching (EGCG20 (53%), EGCG 5 (70%)). Our results seems to be in line with recent publication on C. elegans with no inhibition of egg hatching or larval migration for B-type proanthocyanidins rich extract from Paullinia pinnata roots (Spiegler et al, 2016) or the lifespan and anti-aging effects of EGCG in C. elegans (Brown et al, 2006;Zhang et al, 2009). The effects of the chalcones Phloretin and Phloridzin, a Glycosylated phloretin (Gosch, 2010;Gosch et al) were also screened.…”

Section: Discussionsupporting

confidence: 91%

“…Advances in alternative AHs research head for medicinal plants exploitation (Spiegler et al, 2016). Interest in locally grown plants rich in tannin content, and other potential secondary metabolites with AH properties (Vargas-Magaña et al, 2014) represent an ideal option for Green farming nowadays (Katiki et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Caenorhabditis elegans: a model to study the anthelmintic effects of polyphenolic compounds on the fertility of parasitic gastrointestinal nematodes?

Arroyo-Lopez¹

2020

Preprint

View full text Add to dashboard Cite

We set up a Caenorhabditis elegans model to extrapolate anthelmintic (AH) effects of commercial polyphenols to related gastrointestinal zoonotic species. We compared the practical convenience of S liquid to solid agar Nematode grown medium in daily reproduction patterns and total brood size. Solid agar resulted a highly effective, reproducibly medium, in a less costly and rapid time manner. A minimum of three replications per monomer concentration are suggested to get a robust statistical analysis. Flavonoids Quercetin and Rutin potentially decrease brood sizes and percentage of development, with the exception of 20μM concentration. Flavanols resulted heterogeneous. In a dose-dependent manner Catechin 20μM significantly decreased egg output, and egg hatching in a 15% on respect to control values. Contrary, Epigallocatechin-gallate, seems to stimulate brood sizes and egg-hatching, however, EGCG10μM decreased reproduction but with no in a significative manner. We found a nematocidal effect on the reproductive parameters of C. elegans exposed to the chalcones Phloretin and Phloridzin, and Flavones Flavone and Genistein. A significant general decrease on reproductive parameters were found, particularly significant for Genistein 20μM in the percentage of hatching respect to control. Effects of time schedule and monomer concentration were found for the Hydrolysable tannins Gallic and Tannic acid. Tannic acid showed effectiveness against egg-hatching. The spectrum of percentages of change compared to each blank resulted negative decreasing fertility values, but for GA5, associated with a net increase of larvae hatched.1 AH, GIN, CTS, HTs Q) Quercetin, (R) Rutin, (C) (+)-Catechin hydrate, (EGCG) (-)-Epigallocatechin Gallate, (PTN) Phloretin, (PDN) Phloridzin Dihydrate, (GE) Genistein, (FLA) Flavone, (GA) Gallic acid and (TA) Tannic acid., G1 (adult worm originally seeded per plate and monomer concentration at time 0. Two replications were performed per plate). Solvents: PBS (Phosphate Buffer Solution), CH4O (Methanol 0,002%) L3

show abstract

Section: Discussioncontrasting

confidence: 97%

Section: Discussionsupporting

confidence: 91%

See 1 more Smart Citation

Caenorhabditis elegans: a model to study the anthelmintic effects of polyphenolic compounds on the fertility of parasitic gastrointestinal nematodes?

Arroyo-Lopez¹

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…From the assay, the extract showed lethal activity against T. c a t i (LC 50 = 112 μg/mL), T. vulpis (LC 50 = 17 μg/mL), and C. elegans (LC 50 = 2.5 of mg/mL), but not against A. caninum. Additionally, the effects of the extract on egg hatching and larval migration of the sheep parasite, Haemonchus contortus were investigated in vitro, but no inhibitory activity was observed [59].…”

Section: Paullinia Pinnata L (Euphorbiaceaae)mentioning

confidence: 99%

The Ghanaian Flora as a Potential Source of Anthelmintic and Anti-Schistosomal Agents

Asante-Kwatia¹,

Mensah²,

Gyimah³

et al. 2022

Natural Medicinal Plants

View full text Add to dashboard Cite

Parasitic infections including schistosomiasis and soil transmitted helminthiasis are the most commonly encountered Neglected Tropical Diseases (NTDs) in the world. These diseases remain a major public health concern affecting millions of people especially those living in poor regions where access to effective conventional health care is a challenge. Interventions to control these infections in endemic areas have not been successful due to the high cost of drugs, limited availability as well as inequity of access to preventive chemotherapies. Another problem is the development resistance to the limited number of recommended medications due to their intensive use in both human and live-stock. There is an increasing awareness of the potential of natural products as chemotherapeutic agents to combat parasitic infections. Natural products may offer an unlimited source of chemically diverse drug molecules which may be safe, efficient, less toxic, less expensive and readily available for use especially in low-income countries. The Ghanaian flora provides such a ready source for new therapeutic interventions for the local population. Several researches have provided evidence of the anti-parasitic activity of Ghanaian medicinal plants. This chapter provides a review with special focus on medicinal plants collected from Ghana with anthelmintic and anti-schistosomal activity. Evidence of pharmacological activities of crude extracts, fractions and bioactive phytoconstituents as well as possible mechanisms of action where investigated are discussed.

show abstract

Anthelmintic activity of a traditionally used root extract from Paullinia pinnata

Cited by 2 publications

References 0 publications

Caenorhabditis elegans: a model to study the anthelmintic effects of polyphenolic compounds on the fertility of parasitic gastrointestinal nematodes?

Caenorhabditis elegans: a model to study the anthelmintic effects of polyphenolic compounds on the fertility of parasitic gastrointestinal nematodes?

The Ghanaian Flora as a Potential Source of Anthelmintic and Anti-Schistosomal Agents

Contact Info

Product

Resources

About