Anti-aging and tyrosinase inhibition effects of Cassia fistula flower butanolic extract

Abstract

BackgroundNatural products made from plant sources have been used in a variety of cosmetic applications as a source of nutrition and as a whitening agent. The flowers of Cassia fistula L, family Fabaceae, have been used as a traditional medicine for skin diseases and wound healing and have been reported to possess anti-oxidant properties. The anti-aging effect of C. fistula flower extract on human skin fibroblast was investigated.MethodsThe butanolic extraction of C. fistula flowers was completed and the active compounds were classified. The cytotoxicity of fibroblasts was evaluated by SRB assay for the purposes of selecting non-toxic doses for further experiments. The collagen and hyaluronic acid (HA) synthesis was then measured using the collagen kit and ELISA, respectively. Moreover, the enzyme activity, including collagenase, matrixmelloproteinase-2 (MMP-2) and tyrosinase, were also evaluated.ResultsIt was found that the flower extract did not affect skin fibroblast cell growth (IC50 > 200 μg/mL). The results did show that the flower extract significantly increased collagen and HA synthesis in a dose dependent manner. The flower extract (50–200 μg/mL) also significantly inhibited collagenase and MMP-2 activity. Furthermore, this flower extract could inhibit the tyrosinase activity that causes hyperpigmentation, which induces skin aging.ConclusionsThe C. fistula flower extract displayed a preventive effect when used for anti-aging purposes in human skin fibroblasts and may be an appropriate choice for cosmetic products that aim to provide whitening effects, and which are designated as anti-aging facial skin care products.

Intracellular antioxidant activity of Muntingia calabura leaves methanolic extract

“…The value of cytotoxicity was expressed in IC 50 of 782.115 μg mL -1 . IC 50 values above 200 μg mL -1 show that the extract is not toxic to cells (Limtrakul et al 2016). Based on IC 50 values of antioxidant activity and cytotoxicity, the determination of concentrations of 5, 10, and 20 μg mL -1 can be done for subsequent experiments.…”

“…. Based on Limtrakul et al (2016) the IC 50 value above 200 μg mL -1 indicates that the extract is safe for subsequent experiments because it has low cytotoxicity.…”

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“…Leaves of Lantana camara contains Lantadenes, shows antioxidant activity and acts as free radical scavenging [87] Golden shower tree Cassia fistula L. Flower extract could inhibit the tyrosinase activity that causes hyper-pigmentation, which induces skin aging [88,89] Rubber Bark Tree (Du-Zhong)…”

Synergistic antimicrobial properties of active molecular chitosan with EDTA‐divalent metal ion compounds

“…Even though some materials were quite effective and economically valuable, there were still adverse side effects that able to affect human being directly or indirectly such as causing damage to human body acutely or biomagnification in long term (Fernandez & Gardinali, 2016;Vighi, Matthies, & Solomon, 2016). To overcome these problems, natural resources have been taken at the centre stage as a potently powerful tool related to not only foods (Ahn, Grun, & Mustapha, 2004;Rai, Pandit, Gaikwad, & Kovics, 2016;Sun, Sun, Thavaraj, Yang, & Guo, 2017) but also pharmaceutics (Barbieri et al, 2016;Choi et al, 2017;Leja & Czaczyk, 2016), cosmetics (Limtrakul, Yodkeeree, Thippraphan, Punfa, & Srisomboon, 2016;Pandey, Kumar, Singh, Tripathi, & Bajpai, 2016;Prem, Muangman, Klungsupya, Chantsavang, & Kubera, 2017) and etc.…”