Anti-Aging Effect of Adipose-Derived Stem Cells in a Mouse Model of Skin Aging Induced by D-Galactose

Zhang, Shengchang; Dong, Ziqing; Peng, Zhangsong; Lu, Feng

doi:10.1371/journal.pone.0097573

Cited by 89 publications

(75 citation statements)

References 45 publications

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“…Skin aging is a biological process that induces changes to the structural integrity and physiological function of the skin [1]. Exposure to UV radiation is one of the most significant external stress-inducing factors, and a major cause of premature skin aging.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Oxidative Stress and Skin Aging-Related Enzymes by Prenylated Chalcones and Other Flavonoids from Helichrysum teretifolium

et al. 2015

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Ten flavonoid-related structures viz. heliteretifolin (1), isoxanthohumol (2), 2',4',6'-trihydroxy-3'-prenylchalcone (3), isoglabranin (4), glabranin (5), 7-methoxy-isoglabranin (6), quercetin (7), 4'-methoxyquercetin (8), 4'-methoxykaempferol (9) and mosloflavone (10) were isolated from a H. teretifolium methanolic extract and identified. One of them (compound 1) is reported for the first time from a natural source, while compounds 6, 8–10 were isolated for the first time from the genus Helichrysum. The total extract of H. teretifolium showed potent antioxidant activity. When tested for total antioxidant capacity compound 3 possesses moderate biological activity compared to 2, which displayed some of the highest TEAC values (4529.01 ± 2.44; 4170.66 ± 6.72) µM TE/g, respectively. Compounds 7 and 8 demonstrated the highest inhibitory activities on Fe2+-induced lipid peroxidation (IC50 = 2.931; 6.449 µg/mL); tyrosinase (8.092; 27.573) and elastase (43.342; 86.548). Additionally, the total antioxidant capacities measured as FRAP (4816.31 ± 7.42; 3584.17 ± 0.54) µM AAE/g, and ORAC for hydroxyl radical (7.265 ± 0.71; 6.779 ± 3.40) × 106 and peroxyl radical (17.836 ± 2.90; 12.545 ± 5.07) × 103 µM TE/g were also observed for compounds 7 and 8, respectively. In conclusion, H. teretifolium total extract represents a rich source of bioactive constituents with potent antioxidant and moderate anti-tyrosinase and anti-elastase activities that can help to avert accumulation of free radicals in the body, and could therefore be good candidates for the prevention and/or treatment of skin-related conditions, such as aging. This is the first scientific report on the chemical and biological profile of H. teretifolium.

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Section: Introductionmentioning

confidence: 99%

Inhibition of Oxidative Stress and Skin Aging-Related Enzymes by Prenylated Chalcones and Other Flavonoids from Helichrysum teretifolium

et al. 2015

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“…Remarkably, in our study, the injected CM-Dil-labelled ADSCs were detectable after six weeks. The viability of cells postinjection is critical to the success of injectable cell-based therapies [19]. However, transplanted cells cannot be entirely responsible for the beneficial effect on photoaging; a paracrine effect is more likely to explain the functional results.…”

Section: Discussionmentioning

confidence: 99%

Protective Mechanism of Adipose-Derived Stem Cells in Remodelling of the Skin Stem Cell Niche During Photoaging

Gong¹,

Zhang²,

Li³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Skin photoaging is primarily caused by the functional attrition of skin stem cells. The skin stem cell niche plays an important role in maintaining stem cell survival and behaviour. In our study, we hypothesized that UVB irradiation induces skin photoaging by changing skin stem cell niches and that transferred adipose-derived stem cells (ADSCs) can remodel the niches by affecting the BMP signalling pathway and transdifferentiating into skin stem cells. Methods: Sixty-four C57BL/6J mice were divided into the following groups: a control group, the UVB group and the UVB+ADSCs group. Western blot assays, immunofluorescence analysis and real-time PCR were used to measure differences in the expression of niche components among the three groups. Furthermore, we tested whether transplanted ADSCs express skin stem cell markers, such as p63, α6-integrin and CD34. Results: The expression levels of Bmp4, its downstream factors Smad1 and MAPK1 and a regulatory factor of the niche, i.e., NFATc1, were lower in the UVB group than were those in the control group (P< 0.05) but higher in the UVB+ADSCs group than were those in the UVB group (P< 0.05). Compared with Bmp4, Nanog (a downstream factor of Bmp4), and MMP13 (a regulatory factor of the niche), ICAM-1 (a proinflammatory gene), p63 (a basal transcription factor), β1-integrin, Mtnr1a and Tyr (melanogenesis-related factors) showed the opposite expression trends (P< 0.05). Bmp2 and Collagen IV levels did not significantly change among the three groups (P> 0.05). Skin stem cell markers, such as p63, α6-integrin and CD34, were coexpressed in the ADSCs, which suggested the ADSCs may transdifferentiate into skin stem cells. Conclusion: We found that UVB irradiation results in typical photoaging signs by altering skin stem cell niches and that Bmp4 was a key factor in BMP signalling in hair follicles. ADSCs reversed these typical photoaging signs by remodelling skin stem cell niches through BMP4 pathway modulation and transdifferentiation into skin stem cells.

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“…Evidence from previous studies suggested that ASCs possessed potent antioxidant effects on the epithelial cells. They were found to secrete antioxidant enzymes as superoxide dismutase which scavenged excess ROS in the cells [39] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Adipose-Derived Stem Cells on Induced Photoaging in the Skin of Adult Guinea Pig: Histological and Immunohistochemical Study

Mohammed

Salam

Kalleny

et al. 2017

Egyptian Journal of Histology

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Introduction:The term photoaging describes the sun damaging effects on skin mainly due to chronic ultraviolet (UV) light exposure. The unsatisfactory results of the available anti-aging strategies raise the demand for alternative forms of treatments. Adipose-derived stem cells (ASCs) are available in abundant quantities, harvested by a minimally invasive procedure, safely transplanted and differentiated along multiple cell lineages. Subsequently, used in treatment of many diseases. Aim: To assess the potential ability of ASCs to ameliorate skin changes induced by chronic exposure to artificial light source similar to the sun rays in its UVA and UVB spectrum in adult female guinea pigs. Material and Methods: Adipose-derived stem cells were isolated from subcutaneous white adipose tissue of five adult human donors undergoing elective liposuction surgery. Twenty adult female guinea pigs were used and were randomly divided into two groups each was subdivided into two subgroups "five animals, each". Subgroup IA served as the control group. Subgroup IB was intradermally injected with phosphate buffered saline solution. Subgroup IIA served as the photoaging model. Subgroup IIB served as the photoaging model intradermaly injected with ASCs. Isolated stem cells were cultured and characterized. Skin specimens were prepared and examined using different histological and immunohistochemical techniques. Morphometric and statistical studies were also performed. Results: Subgroup IIA showed various UV damaging effects in the skin epidermis and dermis, while ASCs injection in subgroup IIB resulted in partial restoration of the skin structure. Conclusion: Intradermal injection of ASCs partially ameliorated the photo-damaging effects. Further studies are needed before ASCs clinical application.

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Anti-Aging Effect of Adipose-Derived Stem Cells in a Mouse Model of Skin Aging Induced by D-Galactose

Cited by 89 publications

References 45 publications

Inhibition of Oxidative Stress and Skin Aging-Related Enzymes by Prenylated Chalcones and Other Flavonoids from Helichrysum teretifolium

Inhibition of Oxidative Stress and Skin Aging-Related Enzymes by Prenylated Chalcones and Other Flavonoids from Helichrysum teretifolium

Protective Mechanism of Adipose-Derived Stem Cells in Remodelling of the Skin Stem Cell Niche During Photoaging

Effect of Adipose-Derived Stem Cells on Induced Photoaging in the Skin of Adult Guinea Pig: Histological and Immunohistochemical Study

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