Anti‐apoptotic effect of vinpocetine on cisplatin‐induced hepatotoxicity in mice: The role of Annexin‐V, Caspase‐3, and Bax

Abstract

Hepatic damage is one of the most common complications related to cisplatin (Cis) use. Recently, liver protection lines are being discovered to avoid hepatic cell death as a result of oxidative, inflammatory, and apoptotic disturbance. Limited data reported the hepatoprotective effect of vinpocetine (Vin) in acute liver injury models. This study was designed to determine the potential protective effect of Vin (10-30 mg/kg, orally) against Cis-induced liver injury (10 mg/kg, IP) in mice. Vin administration for 1 week before Cis injection until the end of the experiment. On the 6th day after Cis injection, mice were anesthetized, blood and tissue samples were collected. Hepatic function, histological changes, oxidative stress, inflammation, and apoptotic markers were investigated. Vin administration ameliorated liver injury as indicated by decreased liver injury parameters; serum aminotransferases, ALK-P, GGT, and bilirubin, restored the anti-oxidant status by decrease MDA and NO x , and increased GSH and SOD, inhibited inflammation (IL-6, TNF-α, NFκB-p65, and iNOS) and apoptosis (Annexin-V, Bax, and Caspase-3) parameters. Vin confers dose-dependent protection against Cis-induced liver injury. The hepatoprotective effect of Vin involved anti-oxidative, anti-inflammatory, and anti-apoptotic activities.

Vinpocetine attenuates thioacetamide-induced liver fibrosis in rats

“…These results supported previous notions that backs the hepatoprotective action of Vinpo. 12,13,15,32 Also Vinpo showed no toxic effect on liver markers, and produced no significant change from negative control rat as previous reported. 15 Vinpo at therapeutic doses has shown no significant side effects or toxicity and it is considered a safe drug for long-term use 11…”

Prophylactic and Ameliorative Effects of Vinpocetine Against Lead Induced Hepatotoxicity by Evaluation of Tumor Necrosis Factor-Alpha, Interlukin-10, Interlukin-1beta and Caspase 3

“…The study results are parallel with the results of S. A. Habib and his colleague, regarding reduction of TNFα and caspase 3 after treatment with vinocetine to explore its hepatoprotective activity against cisplatin induced toxicity (13).…”

“…Vinpocetine is a drug with a protective effect to many organs like brain, liver and heart via its voltage gated sodium and calcium channel blockade plus its antioxidant and anti-inflammatory activity (12)(13)(14)(15)(16).…”

Ameliorative Effects of Gallic Acid on Cisplatin-Induced Nephrotoxicity in Rat Variations of Biochemistry, Histopathology, and Gene Expression

“…Some studies report that apoptosis is involved in CP-induced nephrotoxicity [44][45][46]. Some antioxidant compounds prevented CP-induced toxicity such as melatonin [47], selenium [48], vitamin E and vitamin C [43,49,50], some plants [51], modulators of nitric oxide, agents interfering with Cisplatin metabolic pathways, and antiapoptotic and cytoprotective agents [16,[52][53][54].…”