Anti-B-Vitamins

The assimilation of amino acids by bacteria. 24. Inhibitors of incorporation of glycine in disrupted staphylococcal cells

“…It may be that the component of the incorporation mechanism which is inhibited by benzimidazole derivatives is not a purine or purine nucleoside. If we take into account the general principles that have been evolved as a result of studies ofantimetabolites (Woolley, 1952 b;Kodicek 1949) we can deduce that the active component must bear a structural relationship to benzimidazole and that positions 4, 5 and 6 are important in determining its activity. Position 1 is clearly important also since substitution with a-ribofuranosyl, glucopyranosyl or dihydroxypropyl in that position abolishes, whereas substitution with allyl increases, the inhibitory activity of 5:6-dimethylbenzimidazole.…”

The fluorimetric estimation of riboflavin in foodstuffs and other biological material

“…A number of foodstuffs have been assayed both chemically and microbiologically. The latter method is described in a following paper (Kodicek, 1949). Good agreement was found.…”