Anti‐gastric cancer activity and mechanism of natural compound “Heilaohulignan C” isolated from Kadsura coccinea

Abstract

In this research, we analyzed the antitumor activity of one new compound Heilaohulignan C (B‐6) on the human gastric carcinoma cells. MTT, cell migration, Calcein AM/Propidium Iodide (PI), and flow cytometry in BGC‐823 cell line (gastric tumor). Western blot was utilized to distinguish the protein level. Xenografts nude mice were used for in vivo anticancer analysis. H&E staining and laboratory investigation was accomplished for toxicity study. MTT test demonstrated the cytotoxicity of BGC‐823 cells, Calcein AM/Propidium Iodide (PI) examine indicated increment dead cells proportion with a high dose of B‐6, Flow cytometry (FACS) measure showed that B‐6 influenced gastric cancer cells by initiating apoptosis. Western blot analysis confirmed that (B‐6) decrease the level of Bcl‐2 and increase the level of p53, Bax, and cleaved Caspase‐3, this confirms that the B‐6 doing the apoptosis through caspase and cytochrome C apoptotic pathways. Also, B‐6 particularly decline the tumor volume and tumor size in the xenograft mice. H&E staining additionally supports that B‐6 does not have any toxic impact on the normal tissues. This research supports that B‐6 have pharmacological activity against gastric cancer, by p53 and mitochondrial dependent apoptotic pathway, and have no toxicity on normal tissues.

Ursolic acid treats renal tubular epithelial cell damage induced by calcium oxalate monohydrate via inhibiting oxidative stress and inflammation

“…Cytotoxicity was tested via 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay [ 18 ]. The cells transferred to 96-well plates (1 × 10 5 /well) were exposed to serum- and pyruvate-free medium.…”

Kadsura coccinea Lignan Metabolism Based on Metabolome and Transcriptome Analysis

“…Kadsura coccinea is an evergreen woody vine of the Schisandra genus in the Schisandra family, also known as cold rice ball, bufuna, chicken intestine wind, etc. Kadsura coccinea is an important medicinal resource, mainly distributed in Hunan, Guangxi, Guizhou, Fujian, and other places [ 1 ]. Folk medicine mostly uses its roots, vines, and fruits to treat stomach diseases, rheumatoid arthritis, bruises, swelling and pain, irregular menstruation, and so on [ 2 ].…”

“…Folk medicine mostly uses its roots, vines, and fruits to treat stomach diseases, rheumatoid arthritis, bruises, swelling and pain, irregular menstruation, and so on [ 2 ]. Pharmacological studies showed that Kadsura coccinea has the effects of antitumor [ 1 ], antioxidation [ 3 ], liver protection [ 4 ], and inhibition of acetylcholinesterase [ 5 ].…”

Common methods in mitochondrial research (Review)

“…calcein-AM (190,194,198,206,207), in which the acetylmethoxy methyl ester (AM) group enhances the hydrophobicity of the stain for easy penetration of the living cell membrane, is used to fluorescently label living cells. Next, calcein-AM is cleaved by intracellular esterases to yield highly fluorescent and polar calcein (208)(209)(210). When cells are incubated with calcein and co 2+ , both enter the cytoplasm; however, calcein is further captured by mitochondria (211,212).…”