Anti-HIV Immunotoxins

Pincus, Seth H.; Fang, Hua; Wilkinson, Royce A.

doi:10.1007/978-1-4757-4627-3_21

Cited by 1 publication

(1 citation statement)

References 23 publications

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“…Specific efforts include adoptive transfer of T cells, e.g., natural virus-specific T cells expanded ex vivo [ 35 ] or genetically modified T cells expressing cloned T-cell receptors or chimeric antigen receptors [ 36 , 37 ]; another T-cell-mediated technology involves bispecific T-cell-engaging antibodies [ 38 ]. Distinct from these technically complex cell-mediated strategies, RIT technology typically involves intravenous infusion of a purified protein for direct killing of infected cells [ 39 , 40 ]; RITs targeting HIV-1 and other specific viral pathogens have been described [ 41 – 43 ]. We propose that immunotoxins, such as the 6D8-PE38 RIT reported herein, have potential therapeutic utility against EBOV.…”

Section: Discussionmentioning

confidence: 99%

An immunotoxin targeting Ebola virus glycoprotein inhibits Ebola virus production from infected cells

et al. 2021

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Ebola virus (EBOV), a member of the mononegaviral family Filoviridae, causes severe disease associated with high lethality in humans. Despite enormous progress in development of EBOV medical countermeasures, no anti-EBOV treatment has been approved. We designed an immunotoxin in which a single-chain variable region fragment of the EBOV glycoprotein-specific monoclonal antibody 6D8 was fused to the effector domains of Pseudomonas aeruginosa exotoxin A (PE38). This immunotoxin, 6D8-PE38, bound specifically to cells expressing EBOV glycoproteins. Importantly, 6D8-PE38 targeted EBOV-infected cells, as evidenced by inhibition of infectious EBOV production from infected cells, including primary human macrophages. The data presented here provide a proof of concept for immunotoxin-based targeted killing of infected cells as a potential antiviral intervention for Ebola virus disease.

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