Anti-hyperuricemic peptides derived from bonito hydrolysates based on in vivo hyperuricemic model and in vitro xanthine oxidase inhibitory activity

Li, Yujuan; Kang, Xiaoyan; Li, Qingyong; Shi, Chuanchao; Lian, Yingyi; Yuan, Erdong; Zhou, Maojun; Ren, Jiaoyan

doi:10.1016/j.peptides.2018.08.001

Cited by 71 publications

(55 citation statements)

References 25 publications

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“…A positive coefficient indicates that it is positively correlated with the enzyme activity, while a negative number indicates that it is negatively correlated with the enzyme activity. The IC 50 for XO activity increased with increasing peak areas of 3,4,7,10,11,16,17,18,20,21,23,24,25,26,27,28, and 30, indicating that this component group was positively correlated with XO inhibitory activity and that peaks 1,2,5,6,8,9,12,13,14,15,19,22,31,32, and 33 were negatively correlated with their inhibitory activities ( Figure 6). Variable importance in projection (VIP) scores is important indicators that reflect the ability of independent variables to explain dependent variables.…”

Section: Plsr Analysis Resultsmentioning

confidence: 98%

“…These methods enable rapid analysis, complement their respective advantages, and can be used to comprehensively evaluate the quality of L. lucidum. GRA showed that the main components responsible for the XO inhibitory activity of L. lucidum might be characteristic peaks 1,2,4,5,6,7,8,9,11,12,14,15,16,17,20,21,23,25,27,28,29,31,32, and 33. PLS analysis showed that peaks 1, 2, 4, 5, 6, 7, 14, 17, 22, 24, 25, 28, 31, F I G U R E 6 Effect coefficient of each chromatographic peak on the xanthine oxidase inhibitory activity F I G U R E 7 VIP contribution of each characteristic peak to the antioxidant activity of Ligustrum lucidum and 33 had significant effects on the XO inhibitory activity.…”

Section: Plsr Analysis Resultsmentioning

confidence: 99%

“…The gray relational grade is shown in 4,25,26,27,28,29, and 30 was greater than 0.8, indicating that they had a relatively significant effect on the XO activity. Components 1,2,3,5,6,7,8,9,10,11,12,14,15,16,17,18,19,20,21,22,23,24,31,32, and 33 all had correlation degrees greater than 0.7, indicating a significant effect on the XO activity. Combined with the data in the table, it can be seen that the inhibitory activities of characteristic peaks 1,2,4,5,6,7,8,9,11,12,14,15,16,17,20,21,23,25,27,28,29,…”

Section: F I G U R E 3 Cluster Analysis Of the Ligustrum Lucidum Extrmentioning

confidence: 98%

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Study on the spectrum‐effect relationship of the xanthine oxidase inhibitory activity of Ligustrum lucidum

Gao

Chen

Zhou

2019

J of Separation Science

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To evaluate the xanthine oxidase inhibitory activity of the chemical constituents of Ligustrum lucidum in vitro, the spectrum‐effect relationship was investigated. The high‐performance liquid chromatography fingerprint was established by ultraviolet spectrophotometry, and the xanthine oxidase inhibitory activity was tested in vitro by a high‐throughput screening method. Cluster analysis, principal component analysis, gray correlation analysis, and partial least squares regression were used to explore the spectrum‐effect relationships. Sixty batches of Ligustrum lucidum were collected from 16 provinces for testing. The results revealed differences among the batches of medicinal materials, and the similarity score was between 0.635 and 0.968. Thirty‐three characteristic peaks (1–33) were calibrated by fingerprint evaluation software for traditional Chinese medicine. The spectrum‐effect relationship study further revealed that the contents of peaks 1, 2, 4, 5, 6, 7, 14, 17, 25, 28, 31, and 33, which are potentially critical ingredients for quality control of Ligustrum lucidum fruit, were highly correlated with the inhibition of xanthine oxidase activity.

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Section: Plsr Analysis Resultsmentioning

confidence: 98%

Section: Plsr Analysis Resultsmentioning

confidence: 99%

Section: F I G U R E 3 Cluster Analysis Of the Ligustrum Lucidum Extrmentioning

confidence: 98%

See 1 more Smart Citation

Study on the spectrum‐effect relationship of the xanthine oxidase inhibitory activity of Ligustrum lucidum

Gao

Chen

Zhou

2019

J of Separation Science

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“…As previously reported, with minor modifications, the inhibition of XOD activity was quantified by the change of xanthine concentration in the enzyme‐catalysed reaction . All samples were dissolved in phosphate buffer (pH = 7.4, containing 75 mmol/l phosphate ion and 200 μmol/l EDTA).…”

Section: Methodsmentioning

confidence: 88%

“…As previously reported, with minor modifications, the inhibition of XOD activity was quantified by the change of xanthine concentration in the enzyme-catalysed reaction. [23] All samples were dissolved in phosphate buffer (pH = 7.4, containing 75 mmol/l phosphate ion and 200 lmol/l EDTA). The assay mixture: sample solution 200 ll, XOD solution 100 ll (39.2 l/l in the same buffer), all solutions were prepared immediately before use.…”

Section: In Vitro Inhibitory Activity On Xanthine Oxidasementioning

confidence: 99%

Antihyperuricemic and nephroprotective effects of extracts from Orthosiphon stamineus in hyperuricemic mice

Wang

Sun³

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives To investigate the antihyperuricemia and nephroprotective effects of Orthosiphon stamineus extracts on hyperuricemia (HUA) mice and explore the potential mechanisms. Methods Orthosiphon stamineus extracts were extracted using 50% ethanol and enriched using ethyl acetate, and characterised utilising UPLC/ESI‐MS. A potassium oxonate (PO) induced hyperuricemic mouse model was used to evaluate antihyperuricemia and nephroprotective effects of O. stamineus ethyl acetate extracts (OSE). Key findings Eight constituents from OSE were identified and OSE treatment ameliorated HUA by regulating key indicators of kidney dysfunction and xanthine oxidase, adenosine deaminase activity and urate transporters in hyperuricemic mice. Moreover, in renal histopathology analysis, OSE significantly alleviated kidney injury. Conclusions These findings demonstrate that OSE has antihyperuricemic and nephroprotective effects on PO‐induced HUA mice and those results indicate that OSE could be a safe and effective agent or functional ingredient for treating HUA.

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Xanthine oxidase inhibitory peptides derived from tuna protein: virtual screening, inhibitory activity, and molecular mechanisms

Kan

et al. 2020

J Sci Food Agric

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BACKGROUND There has been growing interest in the use of xanthine oxidase (XO) as a therapeutic agent to prevent gout and hyperuricemia. In the present study, XO inhibitory peptides were identified from tuna protein by virtual screening, and molecular docking was used to elicit the interaction mechanism between XO and peptides. RESULTS A novel tetrapeptide, EEAK, exhibited high XO inhibitory activity with an IC50 of 173.00 ± 0.06 μM. Molecular docking analysis revealed that EEAK bound with the pivotal residues of XO's active sites (i.e., Glu802, Arg880, Glu1261) through two conventional hydrogen bond interactions, two attractive charge interactions, and one salt bridge. EEAK could also bind with the residues Phe649, Leu648, Lys771, Ser876, Phe914, and Thr1010 of XO. CONCLUSION This study suggested that conventional hydrogen bond interactions and electrostatic interactions play an important role in XO inhibition. The novel XO inhibitory peptide EEAK from tuna protein could be used as potential candidate for controlling gout and hyperuricemia. © 2020 Society of Chemical Industry

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Anti-hyperuricemic peptides derived from bonito hydrolysates based on in vivo hyperuricemic model and in vitro xanthine oxidase inhibitory activity

Cited by 71 publications

References 25 publications

Study on the spectrum‐effect relationship of the xanthine oxidase inhibitory activity of Ligustrum lucidum

Study on the spectrum‐effect relationship of the xanthine oxidase inhibitory activity of Ligustrum lucidum

Antihyperuricemic and nephroprotective effects of extracts from Orthosiphon stamineus in hyperuricemic mice

Xanthine oxidase inhibitory peptides derived from tuna protein: virtual screening, inhibitory activity, and molecular mechanisms

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