Anti-Inflammatory Activity of Oxyresveratrol Tetraacetate, an Ester Prodrug of Oxyresveratrol, on Lipopolysaccharide-Stimulated RAW264.7 Macrophage Cells

Thaweesest, Wuttinont; Buranasudja, Visarut; Phumsuay, Rianthong; Muangnoi, Chawanphat; Vajragupta, Opa; Sritularak, Boonchoo; Rashatasakhon, Paitoon; Rojsitthisak, Pornchai

doi:10.3390/molecules27123922

Cited by 7 publications

(1 citation statement)

References 64 publications

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“…With a considerable repertoire of pharmacological applications, Morus alba is now the most studied species for oxyresveratrol production (Lim et al, 2015). OxyR presents strong tyrosinase inhibitory activity (J.-K. Kim et al, 2012), together with anti-inflammatory (Thaweesest et al, 2022) and antioxidant (Heo et al, 2016) effects. Its protective properties against neurological diseases have also been reported (Ban et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of oxyresveratrol in mulberry (Morus albaL.) is mediated by a group of p-coumaroyl-CoA 2’-hydroxylases acting upstream of stilbene synthases

Santiago,

Romero,

Martínez

et al. 2024

Preprint

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Mulberry (Morus alba L.) is considered a millenary medicinal plant and a food source for silkworms. Different M. alba extracts offer a variety of biological and pharmacological properties that are in part attributed to stilbenoids, a small group of phenylpropanoids that include resveratrol and oxyresveratrol. These are naturally present in non-renewable parts of mulberry trees, impeding their efficient extraction. As a way to bypass this spatiotemporal restriction, we generated cell suspensions from mulberry twigs and demonstrated that the combined use of methyl jasmonate and methyl- or hydroxypropyl-β-cyclodextrins elicited a high production of resveratrol and oxyresveratrol, both intra and extracellularly. To identify oxyresveratrol-producing enzymes (unknown to date), we first improved the structural and functional annotation of the mulberry genome by integrating short and long-read sequencing data. We further combined this data with transcriptome, metabolite and proteome time-series evidence to identify a complete set of elicited phenylpropanoid- and stilbenoid-related genes. These included 22 stilbene synthase (STS) genes and a group of six p-coumaroyl-CoA 2-hydroxylases (C2Hs) that were highly co-expressed with resveratrol and oxyresveratrol accumulation. We transiently transformed Nicotiana benthamiana plants and grapevine (Vitis vinifera L.) cell suspensions to functionally validate the role of C2Hs as the first committed step of oxyresveratrol synthesis, providing an alternative substrate for STSs by hydroxylating p-coumaroyl-coA into 2,4-dihydroxycinnamoyl-CoA. We offer tools for genomic and transcriptomic exploration in the context of jasmonate elicitation aiding in the characterization of novel stilbenoid-modifying and regulatory genes in the Morus genus.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of oxyresveratrol in mulberry (Morus albaL.) is mediated by a group of p-coumaroyl-CoA 2’-hydroxylases acting upstream of stilbene synthases

Santiago,

Romero,

Martínez

et al. 2024

Preprint

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Bifidobacterium longumBL‐19 inhibits oxidative stress and inflammatory damage in the liver of mice with NAFLD by regulating the production of butyrate in the intestine

Zhang,

Xu,

Dong

et al. 2024

Food Science & Nutrition

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Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease, but there is currently no effective treatment method. Probiotics have been used as an adjunct therapy for NAFLD, but the mechanism is not clear. This study used Bifidobacterium longum BL19 (BL‐19) to treat the NAFLD mice induced by a high‐fat diet, and explored the treatment mechanism through gut microbiota and serum metabolomics techniques. We found that BL‐19 effectively prevented rapid weight gain in NAFLD mice and reduced their overall food and energy intake, decreased liver inflammatory factors expressions, and increased the bile acid synthetase enzyme CYP7A1 and superoxide dismutase. After BL‐19 treatment, the abundances of butyric acid bacteria (Oscillospira and Coprococcus) in the feces of mice increased significantly, and the concentration of butyric acid also increased significantly. We believe that BL‐19 promotes the production of butyrate in the intestines, which in turn regulates the activity of CYP7A1 in the liver and bile acid synthesis, ultimately treating liver inflammation and lipid accumulation in NAFLD mice. Serum metabolomics results indicated that BL‐19 affected multiple pathways related to inflammation and lipid metabolism in NAFLD mice. These findings suggest that BL‐19 shows promise as an adjunct therapy for NAFLD, as it can significantly improve oxidative stress, reduce inflammation in the liver, and decrease lipid accumulation.

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Nutraceutical interventions in Alzheimer's disease

Gujarathi,

Aher,

Sukhia

et al. 2024

Nutraceutical Fruits and Foods for Neurodegenerative Disorders

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Anti-Inflammatory Activity of Oxyresveratrol Tetraacetate, an Ester Prodrug of Oxyresveratrol, on Lipopolysaccharide-Stimulated RAW264.7 Macrophage Cells

Cited by 7 publications

References 64 publications

Biosynthesis of oxyresveratrol in mulberry (Morus albaL.) is mediated by a group of p-coumaroyl-CoA 2’-hydroxylases acting upstream of stilbene synthases

Biosynthesis of oxyresveratrol in mulberry (Morus albaL.) is mediated by a group of p-coumaroyl-CoA 2’-hydroxylases acting upstream of stilbene synthases

Bifidobacterium longumBL‐19 inhibits oxidative stress and inflammatory damage in the liver of mice with NAFLD by regulating the production of butyrate in the intestine

Nutraceutical interventions in Alzheimer's disease

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