Anti-Inflammatory Effects Induced by a Polyphenolic Granular Complex from Olive (Olea europaea, Mainly Cultivar coratina): Results from In Vivo and Ex Vivo Studies in a Model of Inflammation and MIA-Induced Osteoarthritis

Abstract

MOMAST® GR25 is a polyphenolic granular complex from olive pressing juice with high total content in polyphenols. In this work, we evaluated the possible anti-inflammatory effects of MOMAST® GR25 in both acute and chronic inflammatory models. MOMAST® GR25 decreased the levels of prostaglandin (PG) E2 and 8-iso-PGF2α in isolated rat colon, liver, and heart specimens stimulated with lipopolysaccharide (LPS). In vivo, compared to controls, rats treated with MOMAST® GR25 (100 mg/kg to 1 g/kg) showed a significant reduction in both licking/biting time in the formalin test. In a rat model of osteoarthritis by monoiodoacetate (MIA) injection, MOMAST® GR25 showed pain-relieving properties when acutely administered, reducing mechanical hyperalgesia and spontaneous pain. Moreover, a repeated daily treatment with MOMAST® GR25 (300 mg/kg) fully counteracted osteoarticular pain without the development of tolerance to the antinociceptive effect. Taken together, our present findings showed that MOMAST® GR25 could represent a potential strategy for the treatment of inflammation and pain.

Anti-Inflammatory and Antioxidant Effects Induced by Allium sativum L. Extracts on an Ex Vivo Experimental Model of Ulcerative Colitis

“…After collection, isolated colon specimens were maintained in a humidified incubator with 5% CO 2 at 37 °C for 4 h (incubation period) in RPMI buffer with added bacterial LPS (10 μg/mL) [ 26 , 27 ] and treated with either GHE or GWE (1, 10, and 100 μg/mL). Prostaglandin (PG) E 2 and 8-iso-PGF 2α levels (pg/mg wet tissue) were measured by radioimmunoassay (RIA) in tissue supernatants [ 28 , 29 ].…”

Effects of GHRH Deficiency and GHRH Antagonism on Emotional Disorders in Mice

“…One microgram of total RNA extracted from each sample in a 20 µL reaction volume was reverse transcribed using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific Inc., Monza, Italy). The gene expression of COX-2, inducible nitric oxide synthase (iNOS), NF-kB and tumor necrosis factor (TNF)-α in prefrontal cortex specimens was determined by means of quantitative real-time polymerase chain reaction (PCR), using TaqMan probe-based chemistry (Thermo Fisher Scientific Inc., Monza, Italy), as previously reported [16,32]. PCR primers and TaqMan probes were purchased from Thermo Fisher Scientific Inc. (Assays-on-Demand Gene Expression Products, Mm00478374_m1 for COX-2, Mm00443258_m1 for TNF-α, Mm00476361_m1 for NF-kB, Mm00440502_m1 for iNOS, and Mm00607939_s1 for β-actin).…”