Anti-Inflammatory Effects of Hyperbaric Oxygenation during DSS-Induced Colitis in BALB/c Mice Include Changes in Gene Expression ofHIF-1α, Proinflammatory Cytokines, and Antioxidative Enzymes

Abstract

Reactive oxygen species (ROS) and nitrogen species have an indispensable role in regulating cell signalling pathways, including transcriptional control via hypoxia inducible factor-1α (HIF-1α). Hyperbaric oxygenation treatment (HBO2) increases tissue oxygen content and leads to enhanced ROS production. In the present study DSS-induced colitis has been employed in BALB/c mice as an experimental model of gut mucosa inflammation to investigate the effects of HBO2 on HIF-1α, antioxidative enzyme, and proinflammatory cytokine genes during the colonic inflammation. Here we report that HBO2 significantly reduces severity of DSS-induced colitis, as evidenced by the clinical features, histological assessment, impaired immune cell expansion and mobilization, and reversal of IL-1β, IL-2, and IL-6 gene expression. Gene expression and antioxidative enzyme activity were changed by the HBO2 and the inflammatory microenvironment in the gut mucosa. Strong correlation of HIF-1α mRNA level to GPx1, SOD1, and IL-6 mRNA expression suggests involvement of HIF-1α in transcriptional regulation of these genes during colonic inflammation and HBO2. This is further confirmed by a strong correlation of HIF-1α with known target genes VEGF and PGK1. Results demonstrate that HBO2 has an anti-inflammatory effect in DSS-induced colitis in mice, and this effect is at least partly dependent on expression of HIF-1α and antioxidative genes.

Radiation-induced cystitis treated with hyperbaric oxygen therapy (RICH-ART): a randomised, controlled, phase 2–3 trial

“…167 HBOT suppresses inflammatory cytokines, such as TNF production, augments prostaglandin E 2 (PGE 2 ), cyclooxygenase-2 (COX-2), and IFNγ release, and increases the antiinflammatory cytokine IL-10. [168][169][170] Angiogenesis: As previously explained, hypoxia in a cell leads to increased levels of ROS and increased levels of nitric oxide. One of the downstream effects is the increased production of angiogenetic factors, such as HIF-1α and VEGF.…”

The triune of intestinal microbiome, genetics and inflammatory status and its impact on the healing of lower gastrointestinal anastomoses

“…While it might be imagined that hyperbaric oxygen therapy (HBOT) in the animal experiments above acts solely by reversing this hypoxia, the picture is more complex. HBOT in fact also acts in an anti‐inflammatory manner within the intestine, and has been shown to directly block interleukin‐1β (IL‐1β) and increase HIF‐1α mRNA . This is possibly through stabilisation of HIF‐1α via formation of oxygen radicals, but exact mechanisms are as yet unclear .…”

“…HBOT in fact also acts in an anti‐inflammatory manner within the intestine, and has been shown to directly block interleukin‐1β (IL‐1β) and increase HIF‐1α mRNA . This is possibly through stabilisation of HIF‐1α via formation of oxygen radicals, but exact mechanisms are as yet unclear . Other studies of HBOT in the acute inflammatory process also demonstrate a downregulation of cyclooxygenase‐2 (COX2) mRNA , highlighting the anti‐inflammatory role of this therapy.…”

Reduction of TNFR2 Expression in Brain Endothelial Cells in Cerebral Malaria Mice Models after Hyperbaric Oxygen Exposure

“…The administration of HBO can improve the function of macrophages as the main source of TNF-α, where macrophages and monocytes that are exposed to HBO will produce fewer TNF-α than those without HBO exposure. This decrease in proinflammatory cytokine production is associated with decreased NFkB activity and increased HO-1 activity[6]- [7]. Based on the above phenomenon, the goal of this study was to examine the impact of HBO Reduction of TNFR2 Expression in Brain Endothelial Cells in Cerebral Malaria Mice Models after Hyperbaric Oxygen Exposure administration in brain endothelial cells mice with cerebral malaria on TNFR2 expression.…”