Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells

Lee, Seulah; Lee, Dahae; Jang, Tae Su; Kang, Ki Sung; Nam, Joo‐Won; Lee, Hae‐Jeung; Kim, Ki Hyun

doi:10.3390/molecules22101583

Cited by 30 publications

(20 citation statements)

References 36 publications

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“…To examine the effect of FPGE on NO production in macrophages, we measured nitrite (NO 2 ) level using the Griess Reagent System. Previously, we have shown that LPS induces NO production 18 to 24 h after treatment in RAW 264.7 cells [ 21 22 ]. In this study, we also confirmed the induction of NO production by LPS in the macrophage.…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory effects of fermentedPlatycodon grandiflorumextract through NF-κB signaling in RAW 264.7 cells

Park

Lee

2020

Nutr Res Pract

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BACKGROUND/OBJECTIVES: Platycodon grandiflorum (PG), an oriental herbal medicine, has been known to improve liver function, and has both anti-inflammatory and antimicrobial properties. However, little is known about the immune-enhancing effects of PG and its mechanism. In this study, we aimed to investigate whether fermented PG extract (FPGE), which has increased platycodin D content, activates the immune response in a murine macrophage cell line, RAW 264.7. MATERIALS/METHODS: Cell viability was determined by Cell Counting Kit-8 assay and the nitric oxide (NO) levels were measured using Griess reagent. Cytokine messenger RNA levels of were monitored by quantitative reverse transcription polymerase chain reaction. To investigate the molecular mechanisms underlying immunomodulatory actions of FPGE in RAW 264.7 cells, we have conducted luciferase reporter gene assay and western blotting. RESULTS: We found that FPGE treatment induced macrophage cell proliferation in a dosedependent manner. FPGE also modulated the expression of NO and pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, and IL-6. The activation and phosphorylation levels of nuclear factor kappa B (NF-κB) were increased by FPGE treatment. Moreover, 5-aminoimidazole-4-carboxamide ribonucleotide, an activator of AMP-activated kinase (AMPK), significantly reduced both lipopolysaccharides-and FPGE-induced NF-κB reporter gene activity. CONCLUSIONS: Taken together, our findings suggest that FPGE may be a novel immuneenhancing agent acting via AMPK-NF-κB signaling pathway.

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Section: Resultsmentioning

confidence: 99%

Immunomodulatory effects of fermentedPlatycodon grandiflorumextract through NF-κB signaling in RAW 264.7 cells

Park

Lee

2020

Nutr Res Pract

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“…NF-κB is a transcriptional factor that acts as an important mediator of the immune response and controls the expression of several proteins involved in inflammation, such as iNOS and cyclooxygenase-2 (COX-2) [ 15 ]. To further confirm the anti-inflammatory properties of 1 and 3, we investigated their effects on the protein expressions of NF-κB and iNOS in LPS-induced RAW264.7 macrophage cells using Western blotting; these proteins are involved in the pathogenesis of chronic inflammatory diseases [ 16 ]. Consistent with their inhibitory activity toward NO, compounds 1 and 3 inhibited the induction of iNOS and NF-κB protein expression in a dose-dependent manner [ 11 ] ( Figure 4 , Figure 5 , Figure 6 and Figure 7 ).…”

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Triterpenoids from the Caulophyllum robustum Maximin LPS-Stimulated RAW264.7 Cells

et al. 2018

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Caulophyllum robustum Maxim is widely distributed in China and used as a traditional herbal medicine to induce childbirth, ease the pain of labor, rectify delayed or irregular menstruation, alleviate heavy bleeding and pain during menstruation, and treat external injuries and irregular menses. According to our detailed chemical investigation, three new triterpene derivatives (1–3), together with seven known compounds, were isolated from the root and rhizome of C. robustum Maxim. Their structures were elucidated by 1D- and 2D-NMR spectroscopic analysis and physio-chemical methods. They were identified as (1) 23-hydroxy-3,19-dioxo-olean-12-en-28-oic-acid; (2) 23-hydroxy-3,11-dioxo-olean-12-en-28-oic acid; and (3) 16α,23-dihydroxy-3-oxo-olean-12-en-28-oic acid. Compounds (1–10) inhibited the LPS-activated NO production in RAW264.7 cells. Furthermore, the anti-inflammatory characteristics of these compounds were confirmed on the basis of decreases in iNOS and NF-κB protein expression in RAW264.7 cells.

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“…In our ongoing efforts in the discovery of biologically active secondary metabolites from wild Korean mushrooms, we conducted a chemical investigation of C . nipponica and biological evaluation of the isolated compounds .…”

Section: Introductionmentioning

confidence: 99%

Bioactivity‐Guided Isolation of Anti‐Inflammatory Constituents of the Rare Mushroom Calvatia nipponica in LPS‐Stimulated RAW264.7 Macrophages

Lee

et al. 2018

Chemistry & Biodiversity

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Calvatia species, generally known as puffball mushrooms, are used both as sources of food and as traditional medicine. Among the Calvatia genus, Calvatia nipponica (Agaricaceae) is one of the rarest species. Using bioassay-guided fractionation based on anti-inflammatory effects, five alkaloids (1 - 5), two phenolics (6 and 7), and a fatty acid methyl ester (8) were isolated from the fruiting bodies of C. nipponica. Compound 8 was identified from C. nipponica for the first time, and all isolates (1 - 8) were tested for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. Compound 7 showed mild inhibition while compound 8 significantly inhibited NO production with an IC value of 27.50 ± 0.08 μm. The mechanism of NO inhibition of compound 7 was simulated by molecular docking analysis against nitric oxide synthase (iNOS), which revealed the interactions of 7 with the key amino acid residue and the heme in the active site. With the most potent inhibition against LPS-induced inflammation, compound 8 was further investigated with respect to its mechanism of action, and the activity was found to be mediated through the inhibition of iNOS and COX-2 expression.

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Anti-Inflammatory Phenolic Metabolites from the Edible Fungus Phellinus baumii in LPS-Stimulated RAW264.7 Cells

Cited by 30 publications

References 36 publications

Immunomodulatory effects of fermentedPlatycodon grandiflorumextract through NF-κB signaling in RAW 264.7 cells

Immunomodulatory effects of fermentedPlatycodon grandiflorumextract through NF-κB signaling in RAW 264.7 cells

Anti-Inflammatory Triterpenoids from the Caulophyllum robustum Maximin LPS-Stimulated RAW264.7 Cells

Bioactivity‐Guided Isolation of Anti‐Inflammatory Constituents of the Rare Mushroom Calvatia nipponica in LPS‐Stimulated RAW264.7 Macrophages

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