Anti-influenza Viral Effects of Honey In Vitro: Potent High Activity of Manuka Honey

Watanabe, Ken; Rahmasari, Ratika; Matsunaga, Ayaka; Haruyama, Takahiro; Kobayashi, Nobuyuki

doi:10.1016/j.arcmed.2014.05.006

Cited by 132 publications

(125 citation statements)

References 43 publications

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“…To elucidate the inhibitory mechanism of NUD-1 against influenza virus, a time-of-addition assay was performed as described [46], with some modifications. Briefly, MDCK cells (2 × 10 5 cells/well) were seeded in 24-well tissue culture plates and incubated at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Identification of small molecule inhibitors for influenza a virus using in silico and in vitro approaches

et al. 2017

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Influenza viruses have acquired resistance to approved neuraminidase-targeting drugs, increasing the need for new drug targets for the development of novel anti-influenza drugs. Nucleoprotein (NP) is an attractive target since it has an indispensable role in virus replication and its amino acid sequence is well conserved. In this study, we aimed to identify new inhibitors of the NP using a structure-based drug discovery algorithm, named Nagasaki University Docking Engine (NUDE), which has been established especially for the Destination for GPU Intensive Machine (DEGIMA) supercomputer. The hit compounds that showed high binding scores during in silico screening were subsequently evaluated for anti-influenza virus effects using a cell-based assay. A 4-hydroxyquinolinone compound, designated as NUD-1, was found to inhibit the replication of influenza virus in cultured cells. Analysis of binding between NUD-1 and NP using surface plasmon resonance assay and fragment molecular orbital calculations confirmed that NUD-1 binds to NP and could interfere with NP-NP interactions essential for virus replication. Time-of-addition experiments showed that the compound inhibited the mid-stage of infection, corresponding to assembly of the NP and other viral proteins. Moreover, NUD-1 was also effective against various types of influenza A viruses including a clinical isolate of A(H1N1)pdm09 influenza with a 50% inhibitory concentration range of 1.8–2.1 μM. Our data demonstrate that the combined use of NUDE system followed by the cell-based assay is useful to obtain lead compounds for the development of novel anti-influenza drugs.

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Section: Methodsmentioning

confidence: 99%

Identification of small molecule inhibitors for influenza a virus using in silico and in vitro approaches

et al. 2017

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“…The manuka honey, one of the best studied honey types, revealed to be effective against influenza virus in vitro, using Madin-Darby canine kidney cells. 189 Manuka and clover honey were also effective in vitro against varicella zoster virus using human malignant melanoma cells (MeWo). 190 Furthermore, commercial and manuka honey seem to act against herpes simplex virus (HSV-1) isolates in vitro (using Vero cells), 191,192 and honey solutions act against rubella virus also in vitro (using monkey kidney cell cultures).…”

Section: Antiviral Activity Of Honeymentioning

confidence: 99%

Honey as a Complementary Medicine

Miguel

Antunes

Faleiro

2017

Integr Med�Insights

135

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The beneficial effects of honey on human health have long been recognized. Today, many of those positive effects have been studied to elucidate its mode of action. This review briefly summarizes the best studied features of honey, highlighting it as an appealing alternative medicine. In these reports, the health benefits of honey range from antioxidant, immunomodulatory, and anti-inflammatory activity to anticancer action, metabolic and cardiovascular benefits, prebiotic properties, human pathogen control, and antiviral activity. These studies also support that the honey’s biological activity is mainly dependent on its floral or geographic origin. In addition, some promising synergies between honey and antibiotics have been found, as well as some antiviral properties that require further investigation. Altogether, these studies show that honey is effectively a nutraceutical foodstuff.

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“…10 Possui também ação terapêutica antimicrobiana, antiúlcera, 3,4 antitumoral, 11 anti-inflamatória 12 e antiviral. 13 Vale ressaltar que as atividades do mel estão relacionadas a diversos fatores, entre eles seu alto teor de água e açúcares, baixo valor de pH, presença de peróxido de hidrogênio e diversos compostos provenientes do metabolismo secundário vegetal, entre eles os flavonides e os derivados fenólicos. 14,15 O aumento na produção fez com que a comercialização do mel também crescesse e com ela surgissem problemas, como adulteração e contaminação.…”

Section: Introductionunclassified

Evaluation of the Quality of Honey Commercialized in Western Pará, Brazil

Gomes¹,

Dourado²,

Dourado³

et al. 2017

Rev. Virtual Quim.

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Honey is a natural product of high nutritional and therapeutic value. Western of Pará State is a very promising beekeeping region, but the number of dealers who adulterate at least one of the produ t's ph si o he i al hara teristi s i reases as the eekeeping activity grows. Accordingly, the aim of the present study is to assess the quality of 09 samples of Jandaíra (Melipona compressipes manaoensis and Melipona seminigra) honey and 22 samples of Apis mellifera available for sale in west Pará, Brazil. It was observed that only 01 sample of honey from Jandaíra and 11 of Apis mellifera had all the assessed physicochemical parameters in compliance with the Brazilian legislation. With regard to the antioxidant activity against the DPPH radical, most of the samples had maximum inhibition values above 50% and such activity was greater in the darker staining samples. Finally, it was noteworthy that the adulterated honey or the honey outside the standard specifications lost their beneficial properties and shelf life longevity. Thus, its close monitoring in free and local markets is demanding.Keywords: Antioxidant activity; Moisture; Reducing sugars; Ashes; Color. ResumoO mel é um produto natural de alto valor nutricional e terapêutico, sendo a região oeste do Pará muito promissora na atividade apícola. Uma vez que esta atividade vem crescendo, aumenta também o número de comerciantes que adulteram o produto em pelo menos uma de suas características físico-químicas. Neste contexto, no presente trabalho foi avaliada a qualidade de 09 amostras de mel de Jandaíra (Melipona compressipes manaosensis e Melipona seminigra) e 22 amostras de mel de Apis mellifera comercializados na região oeste do Pará, Brasil. Dentre os parâmetros físico-químicos avaliados (cor, pH, teor de umidade, cinzas e açúcares redutores) observou-se que apenas 01 amostra de mel de Jandaíra e 11 de Apis mellifera apresentaram todos os parâmetros físico-químicos avaliados em acordo com a legislação brasileira. Em relação à atividade antioxidante frente ao radical DPPH, a maioria das amostras apresentou valores de inibição máximos acima de 50%, sendo esta atividade maior para as amostras de coloração mais escuras. Por fim, vale ressaltar que méis adulterados ou fora dos padrões implicam na perda de suas propriedades benéficas e diminuição de sua vida de prateleira, logo é necessária uma fiscalização maior do comércio deste produto em feiras e mercados locais.

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Anti-influenza Viral Effects of Honey In Vitro: Potent High Activity of Manuka Honey

Cited by 132 publications

References 43 publications

Identification of small molecule inhibitors for influenza a virus using in silico and in vitro approaches

Identification of small molecule inhibitors for influenza a virus using in silico and in vitro approaches

Honey as a Complementary Medicine

Evaluation of the Quality of Honey Commercialized in Western Pará, Brazil

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