Anti-Obesity Effect of an Ethanol Extract of Cheongchunchal In Vitro and In Vivo

Abstract

Cheongchunchal (CE) is a developed crop more highly enriched in cyanidin-3-O-glucoside chloride (anthocyanin) than conventional waxy corn. Anthocyanin has been proven to have anti-oxidant, anti-inflammatory, anti-obesity, and anti-cancer effects. In this study, using high-performance liquid chromatography (HPLC), Cheongchunchal was confirmed to contain 8.99 mg/g anthocyanin. The inhibitory effect of an ethanol extract of Cheongchunchal (CE) on adipocyte differentiation was demonstrated using Oil Red O staining and a triglyceride assay. By conducting Western blotting, we also confirmed the regulatory effect of CE on adipocyte differentiation factors by assessing changes in the levels of factors that play a significant role in the differentiation of 3T3-L1 preadipocytes. A C57BL/6N mouse model of obesity was induced with a high-fat diet, and CE (400, 600, and 800 mg/kg/day) or Garcinia (245 mg/kg/day) was orally administered to verify the anti-obesity effect of CE. As a result of CE administration, the food efficiency ratio (FER), weight gain, and weight of tissues decreased. Additionally, blood biochemical changes were observed. Furthermore, the inhibitory effect of CE on adipocytes was confirmed through morphological observation and the expression of adipocyte differentiation-related factors in the liver and fat tissues. Therefore, in this study, we verified the anti-obesity effects of anthocyanin-rich CE both in vitro and in vivo.

Anti-Obesity Effect of Hot Water Extract of Barley Sprout through the Inhibition of Adipocyte Differentiation and Growth

“…In addition, the decrease in ALT and AST was also confirmed in the Garciana-cambogia-administered group. Garcinia cambogia has been reported to have hepatotoxicity, for example, causing acute hepatitis, and it is known that the administration of the clinically recommended dose also shows toxicity to the liver in experimental animals [ 20 , 47 , 48 , 49 ]. In this study, it is worth noting that the experiment was conducted by administering a much lower dose of Garcinia cambogia than the dose considering the body surface area.…”

“…In this respect, barley sprouts are noteworthy for their role in healthy functional foods or drugs used for obesity control. Many plant materials are known to help with weight control, and sprouted plants are also known to help with weight control [ 20 , 21 , 22 ]. Sprouted plants are known to have various physiologically active functions because nutrients used for growth after germination are densely concentrated in the plant [ 23 ].…”