Anti-parasitic Antibodies from Phage Display

Goulart, Luíz Ricardo; Ribeiro, Vanessa da Silva; Costa-Cruz, Júlia Maria

doi:10.1007/978-3-319-72077-7_8

Cited by 4 publications

(3 citation statements)

References 105 publications

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“…These methods make it possible to precisely detect the pathogen and determine its taxonomic affiliation. They are constantly being improved in order to increase the speed, sensitivity, and accuracy of the analysis [19][20][21][22][23][24][25]. Despite the high sensitivity and specificity, these methods cannot be considered optimum for mass screening due to their laboriousness, low speed, and high cost of analysis.…”

Section: Introductionmentioning

confidence: 99%

Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry

Grishina

Sherstneva

Grinberg

et al. 2021

Plants

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Chlorophyll fluorescence imaging was used to study potato virus X (PVX) infection of Nicotiana benthamiana. Infection-induced changes in chlorophyll fluorescence parameters (quantum yield of photosystem II photochemistry (ΦPSII) and non-photochemical fluorescence quenching (NPQ)) in the non-inoculated leaf were recorded and compared with the spatial distribution of the virus detected by the fluorescence of GFP associated with the virus. We determined infection-related changes at different points of the light-induced chlorophyll fluorescence kinetics and at different days after inoculation. A slight change in the light-adapted steady-state values of ΦPSII and NPQ was observed in the infected area of the non-inoculated leaf. In contrast to the steady-state parameters, the dynamics of ΦPSII and NPQ caused by the dark–light transition in healthy and infected areas differed significantly starting from the second day after the detection of the virus in a non-inoculated leaf. The coefficients of correlation between chlorophyll fluorescence parameters and virus localization were 0.67 for ΦPSII and 0.76 for NPQ. In general, the results demonstrate the possibility of reliable pre-symptomatic detection of the spread of a viral infection using chlorophyll fluorescence imaging.

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Section: Introductionmentioning

confidence: 99%

Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry

Grishina

Sherstneva

Grinberg

et al. 2021

Plants

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“…Phages have demonstrated their innocuousness for humans, although some concerns still need to be investigated such as the purity of the preparation [ 26 ]. However, this is far from being the only use for phages; they might be a good option to isolate neutralizing antibodies against other infectious diseases, caused by parasites [ 30 ] or viruses [ 31 ], using the phage display technique.…”

Section: Introductionmentioning

confidence: 99%

Viral Related Tools against SARS-CoV-2

Fernández-García

Pacios

González-Bardanca

et al. 2020

Viruses

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At the end of 2019, a new disease appeared and spread all over the world, the COVID-19, produced by the coronavirus SARS-CoV-2. As a consequence of this worldwide health crisis, the scientific community began to redirect their knowledge and resources to fight against it. Here we summarize the recent research on viruses employed as therapy and diagnostic of COVID-19: (i) viral-vector vaccines both in clinical trials and pre-clinical phases; (ii) the use of bacteriophages to find antibodies specific to this virus and some studies of how to use the bacteriophages themselves as a treatment against viral diseases; and finally, (iii) the use of CRISPR-Cas technology both to obtain a fast precise diagnose of the patient and also the possible use of this technology as a cure.

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“…Nanotechnological tools have been applied for the improvement of the conditions of prevention, diagnosis and/or prognosis of diseases (Goulart et al, 2010(Goulart et al, , 2017. In this context, phage display is a high-throughput proteomic technology, in which phagesurface exposed mimotopes recognize specific target ligands.…”

Section: Introductionmentioning

confidence: 99%

High-through identification of T cell-specific phage-exposed mimotopes using PBMCs from tegumentary leishmaniasis patients and their use as vaccine candidates against Leishmania amazonensis infection

et al. 2018

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In the current study, phage-exposed mimotopes as targets against tegumentary leishmaniasis (TL) were selected by means of bio-panning cycles employing sera of TL patients and healthy subjects, besides the immune stimulation of peripheral blood mononuclear cells (PBMCs) collected from untreated and treated TL patients and healthy subjects. The clones were evaluated regarding their specific interferon-γ (IFN-γ) and interleukin-4 (IL-4) production in the in vitro cultures, and selectivity and specificity values were calculated, and those presenting the best results were selected for the in vivo experiments. Two clones, namely A4 and A8, were identified and used in immunization protocols from BALB/c mice to protect against Leishmania amazonensis infection. Results showed a polarized Th1 response generated after vaccination, being based on significantly higher levels of IFN-γ, IL-2, IL-12, tumour necrosis factor-α (TNF-α) and granulocyte-macrophage colony-stimulating factor (GM-CSF); which were associated with lower production of specific IL-4, IL-10 and immunoglobulin G1 (IgG1) antibodies. Vaccinated mice presented significant reductions in the parasite load in the infected tissue and distinct organs, when compared with controls. In conclusion, we presented a strategy to identify new mimotopes able to induce Th1 response in PBMCs from TL patients and healthy subjects, and that were successfully used to protect against L. amazonensis infection.

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Anti-parasitic Antibodies from Phage Display

Cited by 4 publications

References 105 publications

Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry

Pre-Symptomatic Detection of Viral Infection in Tobacco Leaves Using PAM Fluorometry

Viral Related Tools against SARS-CoV-2

High-through identification of T cell-specific phage-exposed mimotopes using PBMCs from tegumentary leishmaniasis patients and their use as vaccine candidates against Leishmania amazonensis infection

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