Anti-silencing function 1B histone chaperone promotes cell proliferation and migration via activation of the AKT pathway in clear cell renal cell carcinoma

Zhou, Jiangqiao; Qiu, Tao; Chen, Zhongbao; Ma, Xiaofen; Zhang, Long; Zou, Jilin; Wang, Tianyu

doi:10.1016/j.bbrc.2019.02.060

Cited by 46 publications

(53 citation statements)

References 23 publications

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“…Bioinformatics analysis was undertaken in accordance with the work of Jiangqiao and collaborators [13].…”

Section: Bioinformatics Analysismentioning

confidence: 99%

“…An overexpressed vector with a flag tag and shRNA vectors of the USP44 (homo) gene were designed and constructed according the method described by Jiangqiao and colleagues [13]. The gene registration number is NM_001347937.1.…”

Section: Lentivirus Of Overexpressed Usp44 and Construction And Prodmentioning

confidence: 99%

“…Cell viability was examined using a CCK-8 kit following manufacturer (44,786; Dojindo, Tokyo, Japan) protocols [13]. 786-O cells and Caki-1 cells were inoculated in 96well plates (167,008; Thermo Scientific, Waltham, MA, USA).…”

Section: Cell Counting Kit (Cck)8 Assaymentioning

confidence: 99%

“…The BrdU experiment was undertaken according to manufacturer (11,647,229,001; Roche, Basel, Switzerland) instructions [13]. 786-O cells and Caki-1 cells were inoculated in 96-well plates.…”

Section: -Bromo-2′-deoxyuridine (Brdu) Experimentsmentioning

confidence: 99%

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Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma

et al. 2020

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Background: Clear cell renal cell carcinoma (ccRCC) is the most common form of adult kidney cancer. Ubiquitinspecific protease (USP)44 has been reported to be involved in various cancers. We investigated the function, role and molecular mechanism of USP44 in ccRCC. Methods: Data obtained from the Cancer Genome Atlas Data Portal and Gene Expression Omnibus database were analyzed to uncover the clinical relevance of USP44 expression and tumor development. USP44 function in the proliferation and migration of tumor cells was assessed by cellular and molecular analyses using ccRCC lines (786-O cells and Caki-1 cells). Results: USP44 showed low expression in ccRCC cancer tissues compared with that in normal tissue. USP44 expression was negatively correlated with tumor stage, tumor grade, and patient survival. USP44 overexpression inhibited the proliferation and migration of 786-O cells and Caki-1 cells significantly. USP44 overexpression also prohibited cell proliferation by upregulating expression of P21, downregulating cyclin-D1 expression, and inhibiting cell migration by downregulating expression of matrix metalloproteinase (MMP)2 and MMP9. USP44 knockdown enhanced the proliferation and migration of 786-O cells and Caki-1 cells. USP44 function in inhibiting the proliferation and migration of 786-O cells and Caki-1 cells was associated with phosphorylation of Jun N-terminal kinase (JNK). Conclusion: USP44 may be a marker in predicting ccRCC progression. Inhibition by USP44 of the proliferation and migration of 786-O cells and Caki-1 cells is dependent upon the JNK pathway.

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“…Bioinformatics analysis was undertaken in accordance with the work of Jiangqiao and collaborators [13].…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Section: Lentivirus Of Overexpressed Usp44 and Construction And Prodmentioning

confidence: 99%

Section: Cell Counting Kit (Cck)8 Assaymentioning

confidence: 99%

“…The BrdU experiment was undertaken according to manufacturer (11,647,229,001; Roche, Basel, Switzerland) instructions [13]. 786-O cells and Caki-1 cells were inoculated in 96-well plates.…”

Section: -Bromo-2′-deoxyuridine (Brdu) Experimentsmentioning

confidence: 99%

See 2 more Smart Citations

Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma

et al. 2020

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“…Bioinformatics analysis was undertaken in accordance with the work of Jiangqiao and collaborators [13]. ccRCC's gene sequence tertiary count data samples and clinical information are obtained through TCGA data portal.…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma

Zhou

Wang

Qiu

et al. 2020

Preprint

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Background: Clear cell renal cell carcinoma (ccRCC) is the most common form of adult kidney cancer. Ubiquitin-specific protease (USP)44 has been reported to be involved in various cancers. We investigated the function, role and molecular mechanism of USP44 in ccRCC. Methods: Data obtained from the Cancer Genome Atlas Data Portal and Gene Expression Omnibus database were analyzed to uncover the clinical relevance of USP44 expression and tumor development. USP44 function in the proliferation and migration of tumor cells was assessed by cellular and molecular analyses using ccRCC lines (786-O cells and Caki-1 cells). Results: USP44 showed low expression in ccRCC cancer tissues compared with that in normal tissue. USP44 expression was negatively correlated with tumor stage, tumor grade, and patient survival . USP44 overexpression inhibited the proliferation and migration of 786-O cells and Caki-1 cells significantly. USP44 overexpression also prohibited cell proliferation by upregulating expression of P21, downregulating cyclin-D1 expression, and inhibiting cell migration by upregulating expression of matrix metalloproteinase (MMP)2 and MMP9. USP44 knockdown enhanced the proliferation and migration of 786-O cells and Caki-1 cells. USP44 function in inhibiting the proliferation and migration of 786-O cells and Caki-1 cells was associated with phosphorylation of Jun N-terminal kinase (JNK). Conclusion: USP44 may be a marker in predicting ccRCC progression. Inhibition by USP44 of the proliferation and migration of 786-O cells and Caki-1 cells is dependent upon the JNK pathway.

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Comprehensive analysis of pan‐cancer reveals potential of ASF1B as a prognostic and immunological biomarker

Zhu

Zhang

et al. 2021

Cancer Medicine

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Background Anti‐silencing function 1 (ASF1) is a conserved histone H3–H4 chaperone protein. ASF1B, a paralog of ASF1, acts by promoting cell proliferation and influencing cell cycle progression. Although there is some evidence demonstrating that ASF1B plays a key role in the development, progression, and prognosis of certain cancers, there are no pan‐cancer analyses of ASF1B. Methods We used a range of bioinformatics approaches to investigate the predictive role of ASF1B, including its correlation with prognosis, tumor mutational burden (TMB), microsatellite instability (MSI), tumor microenvironment (TME), and immune cell infiltration, in diverse cancer types. Results We found that ASF1B was highly expressed in 22 cancers and was negatively correlated with the prognosis of multiple major cancer types. Furthermore, ASF1B expression was correlated with TMB in 21 cancers and with MSI in 7 cancers. We found that ASF1B was coexpressed with genes encoding immune activators, immune suppressors, major histocompatibility complexes, chemokines, and chemokine receptors. We further found that the role of ASF1B in the infiltration of different types of immune cells varied across tumor types. ASF1B may potentially affect several key immune‐related pathways, such as those involved in antigen processing and presentation, natural killer cell‐mediated cytotoxicity, and autoimmune thyroid disease. Conclusions Our findings show that ASF1B may serve as a prognostic marker and potential immunotherapeutic target for several malignancies due to its role in tumorigenesis and immune infiltration.

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Anti-silencing function 1B histone chaperone promotes cell proliferation and migration via activation of the AKT pathway in clear cell renal cell carcinoma

Cited by 46 publications

References 23 publications

Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma

Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma

Ubiquitin-specific protease-44 inhibits the proliferation and migration of cells via inhibition of JNK pathway in clear cell renal cell carcinoma

Comprehensive analysis of pan‐cancer reveals potential of ASF1B as a prognostic and immunological biomarker

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