Antiangiogenic Activity of Mononuclear Copper(II) Polypyridyl Complexes for the Treatment of Cancers

Nagababu, Penumaka; Barui, Ayan Kumar; Bathini, Thulasiram; Devi, Ch. Sarala; Satyanarayana, S.; Patra, Chitta Ranjan; Sreedhar, B.

doi:10.1021/acs.jmedchem.5b00651

Cited by 101 publications

(45 citation statements)

References 52 publications

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“…Experiments were repeated >3 times. To detect ROS production, H 2 O 2 -treated HUVECs (1×10 5 cells) were incubated with the fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate (chloromethyl derivative) at 37°C for 30 min, as previously described ( 23 – 25 ). Subsequently, samples were digested by trypsin-EDTA solution (Beyotime Institute of Biotechnology), centrifuged (1,000 × g for 5 min at room temperature) and transferred to black 96-well plates.…”

Section: Methodsmentioning

confidence: 99%

Roles of miR-4463 in H2O2-induced oxidative stress in human umbilical vein endothelial cells

Wang¹,

He²,

Deng³

et al. 2017

Molecular Medicine Reports

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Oxidative stress is implicated in the pathophysiology of vascular diseases, including atherosclerosis, aneurysm and arteriovenous fistula. A previous study from our lab suggested that microRNA (miR)-4463 may be involved in the pathogenesis of vascular disease; however, the roles of oxidative stress in the molecular mechanisms underlying the actions of miR-4463 in vascular disease have yet to be elucidated. The aim of the present study was to investigate the role of miR-4463 in hydrogen peroxide (H2O2)-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). Reverse transcription-quantitative polymerase chain reaction was used to assess the expression levels of miR-4463 in HUVECs treated with various concentrations of H2O2. Flow cytometry was used to evaluate the percentage of apoptotic cells, and the protein expression levels of the apoptotic markers cleaved (C)-caspase3, poly (adenosine diphosphate-ribose) polymerase 1 (PARP1), B cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax) and X-linked inhibitor of apoptosis protein (XIAP) were determined using western blot analysis. The results demonstrated that the apoptotic rate of HUVECs was increased following treatment with H2O2 in a concentration-dependent manner, and the expression of miR-4463 was also upregulated in a dose-dependent manner. Following transfection with miR-4463 mimics, the levels of malondialdehyde and reactive oxygen species were increased in HUVECs, with a corresponding increase in the apoptotic rate. Furthermore, western blot analysis revealed that the protein expression levels of C-caspase3, PARP1 and Bax were upregulated, whereas the levels of Bcl-2 and XIAP were downregulated. In conclusion, the present findings suggested that the upregulation of miR-4463 may enhance H2O2-induced oxidative stress and promote apoptosis in HUVECs in vitro.

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Section: Methodsmentioning

confidence: 99%

Roles of miR-4463 in H2O2-induced oxidative stress in human umbilical vein endothelial cells

Wang¹,

He²,

Deng³

et al. 2017

Molecular Medicine Reports

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“…Currently, the development of metal complexes with nonplatinum central atoms including ruthenium [11], gold [12], silver [13], copper [14], zinc [15], Manganese [16], nickel [17], iridium [18] and cobalt [19] has been one of the hottest emerging areas of anticancer drug research. Among these, carbonyl cobalt complex has strong properties of cancer prevention including tumour growth/metastasis suppression [20], antiproliferative [21], DNA cleavage [22], apoptotic induction [23] and autophagy [8], which was more active than cisplatin on the human breast tumour cell lines (MCF-7 and MDA-MB-231) [24,25] and ovarian cancer cells (SKOV3/ DDP) [26].…”

Section: Introductionmentioning

confidence: 99%

Co-SLD suppressed the growth of oral squamous cell carcinoma via disrupting mitochondrial function

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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To evaluate the safety and efficacy of novel cobalt complex with sulindac (Co-SLD), the zebrafish and oral squamous cell carcinoma CAL27 were investigated in the present study. The developmental toxicity of Co-SLD ranging from 5 to 20 lM was determined by exposure to 3-144-h post-fertilization (hpf) zebrafish. Our data showed that Co-SLD did not cause to the appreciable toxicity at low concentration (5 and 10 lM). A remarkable toxicity was observed at high concentration (20 lM), including increased mortality and malformation, delayed hatchability, reduced heart rate as well as suppressed behaviour. With regard to the antitumor activity of Co-SLD, inhibited cell growth and migration capability were outstandingly observed in oral squamous cell carcinoma treated with 10 and 20 lM Co-SLD, which could be mainly attributed to the Co-SLD-elicited mitochondrial damage as marked by the depression of mitochondrial membrane potential, ROS accumulation and ATP depletion. Furthermore, administration of 10 lM Co-SLD was an optimal concentration not only to avoid the normal tissue toxicity, but also to enhance the killing of cancer cells via disrupting mitochondrial dysfunction. Taken together the above results demonstrated the desirable response of oral squamous cell carcinoma to Co-SLD. ARTICLE HISTORY

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“…Earlier studies by Sigman in the 1990s [9][10][11][12][13], and by Pitié and Meunier [14][15][16] and several others in recent years [17][18][19][20][21][22][23] indicate efficient DNA cleavage and cytostatic properties. Such compounds are also reported to display antiangiogenic activity [24]. Also, studies by some of us with Cu(II) compounds and tridentate pyridine-based ligands have shown very promising results for both DNA cleavage and anticancer activities [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Transition-metal norharmane compounds as possible cytotoxic agents: New insights based on a coordination chemistry perspective

Khan

Almeida

Al-Farhan

et al. 2016

Journal of Inorganic Biochemistry

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New first-row transition-metal compounds with the ligand norharmane (9H-Pyrido[3,4-b]indole; Hnor) are reported. The compounds have the general formula [M(LL)(Hnor)(NO)](MeOH) (M=Co, Ni, Cu, Zn; LL=2,2'-bipyridyl (bpy), 1,10-phenanthroline (phen)) and have been characterized by physical and analytical methods. X-ray structural analysis revealed that the compound of formula [Cu(phen)(Hnor)(NO)], (1) has a distorted 6-coordinated octahedrally-based geometry, with a planar-based [CuNO] core, where Cu-L varies between 1.99 and 2.04Å and two weak axial CuO contacts (2.209 and 2.644Å) from two different nitrates. Based on spectroscopic similarities, the other compounds appear to have the same or very similar coordination geometries. The compounds showed clear cell growth inhibitory effects in two different cancer cell lines in vitro, with the copper and zinc complexes being the most toxic and in fact almost comparable to cisplatin. Flow-cytometry analysis confirmed induction of apoptosis in cancer cells treated with the compounds. Interestingly, co-incubation of the cells with metal complexes and CuCl induced an increase in the cytotoxic effects, most likely due to the conversion of the metal compounds in the corresponding, and most active, copper analogues.

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Antiangiogenic Activity of Mononuclear Copper(II) Polypyridyl Complexes for the Treatment of Cancers

Cited by 101 publications

References 52 publications

Roles of miR-4463 in H2O2-induced oxidative stress in human umbilical vein endothelial cells

Roles of miR-4463 in H2O2-induced oxidative stress in human umbilical vein endothelial cells

Co-SLD suppressed the growth of oral squamous cell carcinoma via disrupting mitochondrial function

Transition-metal norharmane compounds as possible cytotoxic agents: New insights based on a coordination chemistry perspective

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