Antibacterial Activity and Phytochemical Screening of Mangifera Indica Ethanol and Aqueous Leaves Extract Against Pseudomonas Aeruginosa and Staphylococcus Aureus

Abstract

The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.

In vitro comparative study of the ethanol and aqueous extracts of Spondias mombim leaves, stem bark and charcoal on Streptococcus mutans

“…17 In this study, Spondias mombin stem bark, leaves and charcoal ethanol and aqueous extracts all exhibited antimicrobial activities at varying concentrations (with the exception of 62.5mg/ml of ethanol and aqueous extracts of the stem bark and leaves extracts) in zone of inhibition assay. This result from zone of inhibition was similar to the research carried out by Garga, Garasin 18 and Mada, Garba 19 who reported that the higher the concentrations of both extracts, the higher the antibacterial activities against test organism.…”

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“…Punica granatum plant extract contains terpenoids, steroids, phenols, alkaloids, flavonoids, glycosides, carbohydrates, and protein in different solvent extracts; the same findings were reported by 20 . In Moringa oleifera extract also contains alkaloids, flavonoids, tannins, glycosides, carbohydrates, and phenol were there in different solvent extraction, our results were concurrence with 6 . Alkaloids, flavonoids, steroids, terpenoids, tannins, phenol and protein are there in different solvent extraction of Momordica charantia; similar findings were reported by 7 .…”

“…family and is used to treat various ailments such as diarrhea, common cold, toothache, and oedema 6 . Momordica charantia belong to the Cucurbitaceae family and are used medicinally to treat many diseases like piles, leprosy, jaundice, diabetes, and snake bite 7 .…”

Selection of Effective Plant Extract as A Disinfecting Agent using Hot and Cold-water Extraction

“…In case of M. indica leaves, both the cold and hot water extracts showed sensitive to Staphylococcus aureus, Bacillus sp., P. aeruginosa, Salmonella sp, and E. coli but resistant to Klebsiella sp. and produced a comparatively lesser zone of inhibition than S. cumini leave extracts Garga et al 2020. also reported that water extract of M.indica leaves was sensitive to S.…”