Antibacterial activity of erythrocyte from grass carp (Ctenopharyngodon idella) is associated with phagocytosis and reactive oxygen species generation

Qin, Zhendong; Vijayaraman, Sarath Babu; Lin, Hanzuo; Dai, Yunjia; Zhao, Lijuan; Xie, Jungang; Lin, Weiqiang; Wu, Zhaofei; Li, Jun; Lin, Li

doi:10.1016/j.fsi.2019.06.008

Cited by 36 publications

(35 citation statements)

References 35 publications

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“…In addition to viruses, erythrocytes of Osteichthyes manifest their defense functions against bacteria (13) and fungi (18). The findings of research by Qin et al (13) suggest that RBCs of a grass carp (Ctenopharyngodon idella) interact with such bacteria as Staphylococcus aureus and Escherichia coli.…”

Section: Antibacterial and Antifungal Activity Of Erythrocytes In Fishmentioning

confidence: 99%

“…In Osteichthyes, RBCs can also contribute to elimination of pathogens/antigens, bound in the immune complexes containing the complement, through their recognizing and binding by the complement receptor 1 (CR1) (12). These cells show phagocytic properties (erythrophagocytosis) (12,13), are capable of reacting with various pathogen-associated molecular patterns via pattern recognition receptors, e.g., peptidoglycan recognition proteins, as well as Toll-like receptors (TLRs) and retinoic acid-inducible gene-I-like receptors (RIG-I-like receptors) (2,5,14). Moreover, erythrocytes of Osteichthyes are probably involved in the immune response as the antigen-presenting cells (APCs), via major histocompatibility complex (MHC) class II antigens, and they can contribute to formation of immune synapse with T cells and NK (natural killer) cells (15).…”

Section: Introductionmentioning

confidence: 99%

“…It is worth noting that similar properties, although to a lesser extent and slightly less expressed, are manifested by mammalian (including human) erythrocytes (2,7,13,14,(19)(20)(21). It is known that erythrocytes in these organisms mostly evolved in the way that ensures coverage of the body oxygen demand, losing their nucleus and other cellular organelles, such as mitochondria and Golgi apparatus, and therefore, losing the ability to synthesize biologically active molecules in the immune response.…”

Section: Introductionmentioning

confidence: 99%

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Immune Functions of Erythrocytes in Osteichthyes

et al. 2020

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Red blood cells (RBCs)-erythrocytes-of Osteichthyes are primarily known for their involvement in the process of gas exchange and respiration. Currently, physiological properties of RCBs in fish should also include their ability to participate in defense processes as part of the innate and adaptive immune mechanisms. In response to viruses, bacteria, and fungi or recombinant nanoparticles, they can modulate expression of genes responsible for immune reactions, influence activity of leukocytes, and produce cytokines, antimicrobial peptides, and paracrine intercellular signaling molecules. Via the complement system (CR1 receptor) and owing to their phagocytic properties (erythrophagocytosis), RBCs of Osteichthyes can eliminate pathogens. In addition, they are probably involved in the immune response as antigen-presenting cells via major histocompatibility complex class II antigens.

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Section: Antibacterial and Antifungal Activity Of Erythrocytes In Fishmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immune Functions of Erythrocytes in Osteichthyes

et al. 2020

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“…Together, this information a rms the idea that sh RBCs have a considerable role in innate immunity. In our previous researches, we have reported that RBCs of grass carp showed antibacterial activity which is related to the generation of ROS and phagocytosis [24]. However, the underlying mechanism remains enigmatic.…”

Section: Introductionmentioning

confidence: 99%

Aeromonas Hydrophila Infection Activates Death Receptor Apoptosis Pathway in the RBCs of Grass Carp (Ctenopharyngodon idellus)

Yang

Zhang

et al. 2020

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Background: Fish blood contains RBCs (Red blood cells) as the major cells. Unlike the absence of nucleus in mammalian RBC, fish RBC contains a nucleus. Previous researches have indicated that fish RBCs have a significant function in immune responses. However, the mechanism underlying the immune responses against bacterial infection of teleost RBCs remains enigmatic. To decipher the mechanisms, after the infection of A. hydrophila, transcriptomic profiling of grass carp RBCs was analyzed. Results: Outcomes demonstrated that there were 2144 altogether DEGs (Differently Expressed Genes) between the A. hydrophila non-disease groups, which includes 817 up-regulated and 1327 down-regulated DEGs. Differently Expressed Genes were allocated to 45 GO terms, including 20 natural procedure terms, 14 cell related element, and 11 terms related to molecular functions. Likewise, the expression levels of cytokines by Quantitative Reverse Transcription Polymerase Chain Reaction showed that they (e.g. CCL4, CCL11, CCL20, IL-4, IL-12, and IFNα) were significantly increased after A. hydrophila infection, which was identical to the expression patterns of transcriptomic data. The infection could cause the apoptosis of RBCs which was confirmed by annexin V/PI assay. To further elucidate the apoptosis pathway, the expression levels of genes by mRNA included in cellular apoptosis were monitored. The outcomes indicated that the expressions of mRNA of p53, Fas, Leucine-rich repeats, death domain-containing (LRDD) and caspase 8 were all significantly increased, respectively. Conclusions: Our results support the notion that A. hydrophila infection could activate Fas-related death receptor apoptosis pathway in grass carp RBCs, which will reveal another insight on the mechanisms underlying the antibacterial immunity of teleost fish RBCs.

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“…Antibacterial materials and reactive oxygen species (ROS) can effectively inhibit bacterial colonization [11][12][13]. Metallic ions, such as Ag + , Cu 2+ , and Ni 2+ , as well as reactive oxygen species (ROS) produced by the metal surfaces can destroy the bacterial membrane [14][15][16][17][18]. Additionally, the presence of metal oxides can increase the availability of ROS in liquid environments, thus increasing the bacterial elimination efficiency [19,20].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial performance of nanosecond laser irradiated zirconium-based bulk metallic glass

Wang

Zhang

et al. 2020

Preprint

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Bulk metallic glass (BMG) have attracted considerable attention during the last decades due to their excellent physical, chemical, and biomedical properties. This report aims to investigate the relationship between antibacterial performance, crystallization behavior and laser ablation parameters of Zr-based bulk metallic glass (Zr-BMG) after nanosecond laser irradiation. The surface morphology, crystallization behavior, surface quality, binding energy and Cu/Ni release properties were explored after laser irradiation. The formation of reactive oxygen species following the immersion of Zr-BMG in phosphate-buffered saline was evaluated using the 2',7'-dichlorofluorescin diacetate method. Staphylococcus aureus was selected to assess the antibacterial performance of Zr-BMG and mouse osteoblasts were used to examine the in vitro cytotoxicity. The results showed that at a laser energy intensity lower than 0.08 J/mm2 the amorphous structure of Zr-BMG was retained after the irradiation process. Furthermore, the laser irradiation process considerably increased the antibacterial performance of Zr-BMG. The Cu/Ni release rate, reactive oxygen species concentration, and antibacterial properties were directly proportional to the laser energy intensity. However, surfaces with a high antibacterial rate also showed high cytotoxicity. The surface irradiated with 7 μJ ablation pulse and 200 mm/s irradiation speed showed a better balance between antibacterial and cytotoxic properties with amorphous status.

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Antibacterial activity of erythrocyte from grass carp (Ctenopharyngodon idella) is associated with phagocytosis and reactive oxygen species generation

Cited by 36 publications

References 35 publications

Immune Functions of Erythrocytes in Osteichthyes

Immune Functions of Erythrocytes in Osteichthyes

Aeromonas Hydrophila Infection Activates Death Receptor Apoptosis Pathway in the RBCs of Grass Carp (Ctenopharyngodon idellus)

Antibacterial performance of nanosecond laser irradiated zirconium-based bulk metallic glass

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