Antibacterial activity of phellodendron bark against <i>Streptococcus mutans</i>

Tsujii, Toshiya; Kawada‐Matsuo, Miki; Migita, Hirono; Ohta, Kouji; Oogai, Yuichi; Yamasaki, Yuichi; Komatsuzawa, Hitoshi

doi:10.1111/1348-0421.12787

Cited by 10 publications

(5 citation statements)

References 48 publications

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“…Moreover, if the plant extract is combined with conventional cytostatic drugs, the side effects caused by the drug are also reduced [ 54 ]. Similar to B. vulgaris and M. aquifolium , P. amurense was also reported to have good anticancer potential [ 12 ] and other important pharmacological applications [ 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Potential of Three Berberine-Containing Plant Extracts Obtained from Berberis vulgaris L., Mahonia aquifolium (Pursh) Nutt., and Phellodendron amurense Rupr

Ciorîță,

Erhan,

Soran

et al. 2024

Biomedicines

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Three berberine-containing plant extracts were investigated for their pharmacological properties. The stems and leaves of Berberis vulgaris, Mahonia aquifolium, and Phellodendron amurense were characterized through scanning electron microscopy. The plant extracts obtained from fresh stem barks were further analyzed through high-performance liquid chromatography, revealing berberine concentrations, among berbamine and palmatine. The plant extracts were further tested for their anticancer potential against 2D and 3D human skin melanoma (A375) and lung adenocarcinoma (A549) cell lines. The concentrations at which 50% of the cells are affected was determined by the viability assay and it was shown that B. vulgaris, the plant extract with the highest berberine concentration, is the most efficient inhibitor (0.4% extract concentration for the 2D model and 3.8% for the 3D model). The membrane integrity and nitrate/nitrite concentration assays were consistent with the viability results and showed effective anticancer potential. For further investigations, the B. vulgaris extract was used to obtain silver nanoparticles, which were characterized through transmission electron microscopy, energy dispersive spectroscopy, and X-ray diffraction. The formed nanoparticles have a uniform size distribution and are suited for future investigations in the field of biomedical applications, together with the B. vulgaris plant extract.

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Section: Discussionmentioning

confidence: 99%

Pharmacological Potential of Three Berberine-Containing Plant Extracts Obtained from Berberis vulgaris L., Mahonia aquifolium (Pursh) Nutt., and Phellodendron amurense Rupr

Ciorîță,

Erhan,

Soran

et al. 2024

Biomedicines

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“…The extract showed higher activity against S. Mutans at 100%. Streptococcus Mutans is the major pathogen responsible for dental caries which has ability to produce biofilms (19).According to our knowledge, currently this is the first study the minimum inhibitory concentration and minimum bacterial concentration of Pithecellobium dulce was evaluated against Streptococcus mutans.…”

Section: Mbc By Agar Dilution Methodsmentioning

confidence: 99%

Evaluation of antibacterial potential of Pithecellobium dulce against Streptococcus mutans

2023

JPTCP

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Introduction: Pithecellobium dulce is used as traditional medicine. It is being utilized as an antibacterial agent against various oral diseases. Streptococcus Mutans is the major causative bacteria for dental caries. This study aims to evaluate the antibacterial potential of Pithecellobium Dulce on Streptococcus Mutans. Aim: This study aims to evaluate the antimicrobial activity of Pithecellobium Dulce against Streptococcus Mutans. Methodology: The minimum inhibitory concentration of Streptococcus mutans was evaluated by the microdilution method utilizing spectrophotometer. The Pithecellobium dulce extract was tested with 25µl, 50 µl, 100 µl concentration, standard and positive control at different time intervals 1hr, 2hr, 3hr, and 4 hr. The minimum bactericidal concentration was evaluated by the agar plate diffusion method at 25µl, 50 µl and 100 µl concentrations and no colonies were counted. Results: The growth of Streptococcus Mutans bacteria was inhibited and at each concentration (25µl, 50 µl and 100 µl) of the extracts showed significant inhibition in comparison to the positive control. The minimum bactericidal concentration was tested and at 100 µl concentration minimum bacterial count was obtained. Conclusion:The findings suggest that Pithecellobium dulce extract has potential to be used as an alternative to antimicrobial agent against Streptococcus mutans. Clinical significance: Antibiotics have been known to cause side effects such as premature death, antibiotic resistance, weakened immune system, and allergy. Hence, there is a need to develop new antibiotics from natural sources

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“…The biofilm formation assay was performed with the method described elsewhere. 30 An overnight culture of S. mutans strains was adjusted to an OD 660 of 1.0, and the bacterial culture was diluted 100-fold (10 7 cells/mL). Ten microliters of bacterial culture was added to each well containing 100 μL of TSB with 1% sucrose in a 96-well plate.…”

Section: Biofilm Formationmentioning

confidence: 99%

Comprehensive characterization of sortase A‐dependent surface proteins in Streptococcus mutans

Katsumata

Kawada‐Matsuo

et al. 2022

Microbiology and Immunology

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Streptococcus mutans, a cariogenic pathogen, adheres to the tooth surface and forms a biofilm. Bacterial cell surface proteins are associated with adherence to substrates. Sortase A (SrtA) mediates the localization of proteins with an LPXTG motifcontaining proteins to the cell surface by covalent binding to peptidoglycan. In S. mutans UA159, six SrtA-dependent proteins, SpaP, WapA, WapE, DexA, FruA, and GbpC, were identified. Although some of these proteins were characterized, a comprehensive analysis of the six proteins has not been reported. In this study, we constructed mutants deficient in each of these proteins and the SrtA-deficient mutant. The SrtA-deficient mutant showed drastically decreased binding to salivary components, biofilm formation, bacterial coaggregation activity, hydrophobicity, and cellular matrix binding (collagen type I, fibronectin, and laminin). The SpaP-deficient mutant showed significantly reduced binding to salivary components and partially increased coaggregation with Porphyromonas gingivalis, and decreased hydrophobicity, and collagen binding. The WapA-deficient mutant showed slightly decreased coaggregation with Fusobacterium nucleatum. Although the SrtA-deficient mutant showed drastically altered phenotypes, all SrtA-dependent protein-deficient mutants, except the SpaP-deficient mutant, did not show considerable alterations in binding to salivary components. These results indicate that the six proteins may coordinately contribute to these activities. In addition, using genomic data of 125 S. mutans strains, the amino acid sequences of each surface protein were compared and many variations were found among strains, which may affect the phenotype of cell surface proteins in S. mutans.

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Antibacterial activity of phellodendron bark against Streptococcus mutans

Cited by 10 publications

References 48 publications

Pharmacological Potential of Three Berberine-Containing Plant Extracts Obtained from Berberis vulgaris L., Mahonia aquifolium (Pursh) Nutt., and Phellodendron amurense Rupr

Pharmacological Potential of Three Berberine-Containing Plant Extracts Obtained from Berberis vulgaris L., Mahonia aquifolium (Pursh) Nutt., and Phellodendron amurense Rupr

Evaluation of antibacterial potential of Pithecellobium dulce against Streptococcus mutans

Comprehensive characterization of sortase A‐dependent surface proteins in Streptococcus mutans

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