Antibacterial Activity of the Ethanolic Extracts Derived from Leaves and Pseudobulbs of Some Orchids Belonging to Coelogyne Genus Against Enterobacter Cloacae Strain

Buyun, Lyudmyla; Tkachenko, Halyna; Kurhaluk, Natalia; Gyrenko, Oleksandr; Kovalska, Lyudmyla; Góralczyk, Anna; Томин, В. И.; Osadowski, Zbigniew

doi:10.15414/agrobiodiversity.2019.2585-8246.348-360

Cited by 2 publications

(1 citation statement)

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“…Previously, we have given considerable attention to the evaluation of the antibacterial activity of ethanolic extracts derived from leaves and pseudobulbs of plants belonging to various Coelogyne species, maintained under glasshouse conditions (Buyun et al, 2016(Buyun et al, -2019a(Buyun et al, ,b, 2021. For example, the assessment of the antifungal potential of orchids species, i.e.…”

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confidence: 99%

Oxidative Stress Biomarkers in Equine Erythrocytes and Plasma after in vitro Treatment with an Aqueous Leaf Extract of Coelogyne brachyptera Rchb. f. (Orchidaceae)

Buyun¹

2022

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Many orchids have been used as medicinal plants for many years in China, Japan, India, and some other countries. They showed many health-beneficial functions, such as the protection of cells against free radicals and oxidative stress possessing hepatoprotection, cardioprotection, gastroprotection, neuroprotection, and other properties. This study aimed to investigate the oxidative stress biomarkers [2-thiobarbituric acid reactive substances (TBARS), aldehydic and ketonic derivatives of oxidatively modified proteins (OMP), and total antioxidant capacity (TAC)] in the equine erythrocytes and plasma after in vitro incubation with an extract derived from leaves of Coelogyne brachyptera Rchb. f. The leaves of C. brachyptera plants, cultivated under glasshouse conditions, were sampled at M.M. Gryshko National Botanic Garden (NBG), National Academy of Science of Ukraine. Freshly collected leaves were washed, weighed, crushed, and homogenized in 0.1M phosphate buffer (pH 7.4) (in the proportion of 1:19, w/w). The equine plasma and erythrocyte aliquots were used in the study. The pellet of blood was re-suspended in phosphate buffer (pH 7.4). A volume of 0.1 ml of the C. brachyptera extract was added to 1.9 ml of clean equine erythrocytes or 1.9 mL of plasma. For positive control (blank), 0.1M phosphate buffer (pH 7.4) added to erythrocytes or plasma was used. Results of our study revealed that erythrocytes were more sensitive to the action of an extract derived from leaves of C. brachyptera. The levels of aldehydic and ketonic derivatives of oxidatively modified proteins in the treated erythrocytes were significantly decreased, while these parameters were no-changed in the equine plasma. The treatment of equine erythrocytes by extract derived from leaves of C. brachyptera increased lipid peroxidation. On the other hand, plasma TBARS level after treatment by extract derived from leaves of C. brachyptera was at the same level as in untreated controls. The level of total antioxidant capacity was not-significantly changed after treatment both in equine plasma and erythrocytes. Studies concerning the antioxidant properties of orchids are continued in our laboratory.

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Section: Introductionmentioning

confidence: 99%

Oxidative Stress Biomarkers in Equine Erythrocytes and Plasma after in vitro Treatment with an Aqueous Leaf Extract of Coelogyne brachyptera Rchb. f. (Orchidaceae)

Buyun¹

2022

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The Antimicrobial Activity of Ethanolic Extracts Derived From Leaves and Pseudobulbs of Coelogyne Flaccida Lindl. (Orchidaceae) Against Different Staphylococcus Aureusstrains

Buyun¹,

Kovalska²,

Gyrenko³

et al. 2020

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The present study was conducted to investigate in vitro antimicrobial activity of ethanolic extracts obtained from leaves and pseudobulbsof Coelogyneflaccida against Staphylococcus aureus subsp. aureus Rosenbach (ATCC®25923™) (mecA negative), Staphylococcus aureus subsp. aureus Rosenbach (ATCC®29213™)(mecA negative, Oxacillin sensitive, weak β-lactamase-producing strain), Staphylococcus aureus NCTC 12493 (mecA positive, Methicillin-resistant, EUCAST QC strain for cefoxitin).The antimicrobial susceptibility testing was done on Muller-Hinton agar by the disc diffusion method (Kirby-Bauer disk diffusion susceptibility test protocol). The leaves and pseudobulbs of C. flaccida plants, cultivated under glasshouse conditions, were sampled at M.M. Gryshko National Botanic Garden (NBG), National Academy of Science of Ukraine. Freshly leaves and pseudobulbs were washed, weighed, crushed, and homogenized in 96% ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and investigated for their antimicrobial activity. The cultivation medium was trypticase soy agar (Oxoid™, UK), supplemented with 10% defibrinated sheep blood. Cultures were grown aerobically for 24 h at 37°C. The cultures were later diluted with a sterile solution of 0.9% normal saline to approximate the density of 0.5 McFarland standard. The McFarland standard was prepared by inoculating colonies of the bacterial test strain in sterile saline and adjusting the cell density to the specified concentration. The examined ethanolic extracts obtained from leaves and pseudobulbs of C. flaccida showed remarkable antibacterial activities against different S. aureusstrains. The observed activity may contribute to the reasons why orchids are used for infectious and inflammatory conditions in ethnomedicine. The research showed that ethanolic extracts of C. flaccida possess the highest antibacterial potency against S. aureus NCTC 12493 strain. These findings led the authors to suggest that these extracts may be used as natural antiseptics and antimicrobial agents in medicine and veterinary practice. Nevertheless, despite the promising results, more research should be carried out to further evaluate the roles of particular compounds, isolated from all parts of orchid plants, attributable to antimicrobial activity.

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Antibacterial Activity of the Ethanolic Extracts Derived from Leaves and Pseudobulbs of Some Orchids Belonging to Coelogyne Genus Against Enterobacter Cloacae Strain

Cited by 2 publications

References 20 publications

Oxidative Stress Biomarkers in Equine Erythrocytes and Plasma after in vitro Treatment with an Aqueous Leaf Extract of Coelogyne brachyptera Rchb. f. (Orchidaceae)

Oxidative Stress Biomarkers in Equine Erythrocytes and Plasma after in vitro Treatment with an Aqueous Leaf Extract of Coelogyne brachyptera Rchb. f. (Orchidaceae)

The Antimicrobial Activity of Ethanolic Extracts Derived From Leaves and Pseudobulbs of Coelogyne Flaccida Lindl. (Orchidaceae) Against Different Staphylococcus Aureusstrains

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