Antibacterial and Resistance Modifying Activities of <i>Nigella sativa</i> Essential Oil and its Active Compounds Against <i>Listeria monocytogenes</i>

Mouwakeh, Ahmad; Telbisz, Ágnes; Spengler, Gabriella; Mohácsi-Farkas, Csilla; Kiskó, Gabriella

doi:10.21873/invivo.11302

Cited by 36 publications

(25 citation statements)

References 24 publications

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“…N. sativa showed antibacterial effect against Salmonella , Helicobacter pylori , and Escherichia coli strains (Bakal, Bereswill, & Heimesaat, ). In addition, Listeria monocytogenes proved to be susceptible toward N. sativa EO, Thq, and Car (Mouwakeh, Telbisz, Spengler, Mohácsi‐Farkas, & Kiskó, ). It has been demonstrated that N. sativa EO was effective against Salmonella enterica causing the food‐borne disease salmonellosis (Ashraf et al, ).…”

Section: Discussionmentioning

confidence: 99%

Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus

Mouwakeh

Kincses

Nové

et al. 2019

Phytotherapy Research

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Nigella sativa essential oil (EO) and its compounds (thymoquinone, carvacrol, and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol, and p-cymene against one methicillin susceptible and one methicillin resistant Staphylococcus aureus strain. N. sativa EO, thymoquinone, carvacrol, and p-cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of mepA gene (by real-time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE/DEAD BacLight™ Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of S. aureus were susceptible to N. sativa EO, thymoquinone, and carvacrol. N. sativa EO and carvacrol induced the increase of EtBr accumulated by both S. aureus strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p-cymene, whereas for the methicillin resistant S. aureus (MRSA) strain the membrane integrity was disrupted by each compound. N. sativa EO and its bioactive compounds such as carvacrol and p-cymene could be applied as resistance modifiers in MRSA strains.

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Section: Discussionmentioning

confidence: 99%

Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus

Mouwakeh

Kincses

Nové

et al. 2019

Phytotherapy Research

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“…Efflux pumps are transport protein in bacteria associated with elimination of toxic molecules from cell to external environment, moreover, this mechanism play essential role in reducing bacteria killing and develop bacteria resistance. The killing mechanism of Nigella Sativa extract on Serratia marcesence could be involved directly in the effect on cell membrane efflux pumps, which reduce bacteria resist and survive [39,40].…”

Section: Resultsmentioning

confidence: 99%

Investigating the Efficiency of Simple Aqueous Extract of Nigella Sativa Activity Against Serratia Marcescens Bactria

Shkhaier

Majid

Alawi

et al. 2020

MJS

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Nigella sativa seeds is considered as a very effective treatment for a variety of human diseases in traditional medicine worldwide with low human toxicity. Serratia marcescens pathogenic species, a Gram-negative, opportunistic bacteria, are widespread and distributed in the human environment with considered resistance to different types of antibiotics. Extraction was performed on 12.5 gm. /150 ml of Nigella seeds using rotary evaporator. Different time periods for extraction were applied (1-4 hours) with different temperature ranges of 50 °C to 60 °C performed separately for each time period. The 3 and 4 hours extraction period at 60 °C for seeds was effective against Serratia marcescens with inhibition zone of 11.18 and 12.27 mm respectively. Nigella seeds could be a promising inhibitor with enhanced activity against Serratia marcescens. Water extraction was efficient and effective at 60 °C, with extraction period of 2-3 hours for seeds. No activity recorded below this temperature.

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“…Moreover, all of the bioactive constituents (p-cy, Thq, Car, Thy) showed a direct antibacterial effect against C. trachomatis D. As only one study related to anti-chlamydial activity of EOs has been published, further studies are needed to clarify the exact mechanisms of their effects. Car and Thq were able to damage the cell membrane of S. aureus and Listeria monocytogenes [14,26]. Thy, which is the most common constituent of EOs obtained from Thymus spp.…”

Section: Discussionmentioning

confidence: 99%

“…N. sativa EO extraction was performed as reported earlier [14]. Thymoquinone (Thq), thymol (Thy), and carvacrol (Car) were purchased from MilliporeSigma ( St. Louis, MO, USA) and p-cymene (p-cy) was purchased from Alfa Aesar (Haverhill, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Bioactive Compounds of Nigella Sativa Essential Oil as Antibacterial Agents against Chlamydia Trachomatis D

et al. 2019

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Urogenital tract infection caused by obligate intracellular bacterium Chlamydia trachomatis D (CtrD) is a leading cause of sexually transmitted diseases. Essential oil (EO) of Nigella sativa has a broad antimicrobial spectrum. The aim of this study was to evaluate the antimicrobial activity of the bioactive compounds (p-cymene, thymoquinone, carvacrol, and thymol) of N. sativa EO against CtrD. The cytotoxic effects of the compounds were determined by MTT assay. In order to quantify the anti-chlamydial activity of the compounds, HeLa cells were infected with CtrD or CtrD treated previously with the compounds. The titer of the infectious CtrD was determined by indirect immunofluorescence assay. The minimum inhibitory concentrations of the compounds were evaluated by direct quantitative PCR. None of the compounds showed a cytotoxic effect on HeLa cells in the concentrations tested. According to the immunofluorescence assay, all of the compounds significantly inhibited the growth of CtrD. The quantitative PCR revealed that the minimum concentration that exerted anti-chlamydial activity was 3.12 µM in the case of thymoquinone and p-cymene, while that of carvacrol and thymol was 6.25 µM. Therefore, it can be concluded that bioactive compounds of N. sativa EO could be used as effective antimicrobial agents against CtrD.

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Antibacterial and Resistance Modifying Activities of Nigella sativa Essential Oil and its Active Compounds Against Listeria monocytogenes

Abstract: Abstract. Background/Aim: N. sativa essential oil (EO) and its compounds (thymoquinone, carvacrol and p-cymene)

Cited by 36 publications

References 24 publications

Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus

Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus

Investigating the Efficiency of Simple Aqueous Extract of Nigella Sativa Activity Against Serratia Marcescens Bactria

Bioactive Compounds of Nigella Sativa Essential Oil as Antibacterial Agents against Chlamydia Trachomatis D

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