Antibacterial and Smear Layer Removal Efficacy of Er:YAG Laser Irradiation by Photon-Induced Photoacoustic Streaming in Primary Molar Root Canals: A Preliminary Study

Korkut, Emre; Torlak, Emrah; Gezgin, Onur; Özer, Hazal; Şener, Yağmur

doi:10.1089/pho.2017.4369

Cited by 34 publications

(20 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The laser energy exploited with the LAI (laser-activated irrigation) methods has been claimed to improve the effectiveness of endodontic irrigants [11,24,25]. The recent PIPS laser technique has the peculiarity of being based on sub-ablative photoacoustic rather than photothermal phenomena, lowering the risk of scaling the dentinal surface [26,27]. The low energy levels (between 20 and 50 mJ), with a frequency between 10 and 15 Hz and with very short pulses (50 ms), generate photoacoustic shockwaves [17].…”

Section: Discussionmentioning

confidence: 99%

Influence of Photon-Induced Photoacoustic Streaming (PIPS) on Root Canal Disinfection and Post-Operative Pain: A Randomized Clinical Trial

Mandras

Pasqualini

Roana

et al. 2020

JCM

View full text Add to dashboard Cite

The aim of this study was to evaluate the ability of a PIPS (photon-induced photoacoustic streaming) Er:YAG laser to reduce the root canal system bacterial count in vivo in comparison to the traditional irrigation technique. The post-operative patients’ quality of life (QoL) after endodontic therapy was evaluated through a questionnaire. Fifty-four patients affected by pulp necrosis with or without apical periodontitis biofilm disease were selected for endodontic treatment and randomly assigned to Group A (n = 27) with traditional irrigation and Group B (n = 27), with PIPS irrigation applied according to the protocol. Shaping was performed with ProGlider and ProTaper Next, and irrigation was performed with 5% NaOCl and 10% EDTA. Intracanal samples for culture tests were collected before and after irrigation. The microbiological analysis was evaluated by the Kolmogorov–Smirnov normality and Mann–Whitney tests (p < 0.05). A self-assessment questionnaire was used to evaluate the QoL during the 7 days after treatment; differences were analysed with Student’s t-test. Irrigation with the PIPS device was significantly effective in reducing bacterial counts, which were higher for facultative than obligate anaerobic strains, particularly for Gram-negative bacteria, without statistical significance (p > 0.05). There were no significant differences among the QoL indicators, except for the maximum pain (p = 0.02), eating difficulty (p = 0.03) and difficulty performing daily functions (p = 0.02) in the first few days post-treatment. PIPS may represent an aid to root canal disinfection not affecting the patients’ QoL, particularly for the first day after treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of Photon-Induced Photoacoustic Streaming (PIPS) on Root Canal Disinfection and Post-Operative Pain: A Randomized Clinical Trial

Mandras

Pasqualini

Roana

et al. 2020

JCM

View full text Add to dashboard Cite

show abstract

“…Nevertheless, since bacterial cells in biofilms are more resistant to disinfectants and physical effects compared with their planktonic counterparts, clinicians face a challenge in elimination of those from the root canals (Korkut, Torlak, Gezgin, Ozer, & Sener, ; Torlak & Sert, ). Considering that confocal microscopy cannot visualize the biofilm at the root canal due to high thickness of the sample of >5 mm, we used green fluorescence under fluorescence microscope as the staining method for detection (Mohmmed et al, ).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of the photon‐initiated photoacoustic streaming combined with different solutions on Enterococcus faecalis in the root canals

Wen

Kong

Zhao

et al. 2020

Microscopy Res & Technique

View full text Add to dashboard Cite

This study aimed to evaluate the efficacy of different irrigation solutions used in photon-initiated photoacoustic streaming (PIPS) or conventional needle irrigation (CNI) for eradication of Enterococcus faecalis from artificial root canals. Altogether, 240 artificial root canal samples were included. The models were split and incubated for 2 days to allow formation of E. faecalis biofilm. The models were randomly divided into two groups (n = 120): CNI and laser-activated irrigation (LAI). Each group was divided into six subgroups according to different irrigation solutions: distilled water, 1% sodium hypochlorite (NaOCl), 2% NaOCl, 5.25% NaOCl, MTAD, and chlorhexidine, respectively. After irrigation, half of the samples (n = 10) were assessed immediately, and the other half of the samples (n = 10) were incubated for 6 hr. Bacterial suspensions were obtained from all samples before and after irrigation, and after incubation, and were quantified adenosine 5 0 -triphosphate (ATP) assay kit. The biofilms were examined using fluorescent microscopy and analyzed by Image Pro Plus software. Significant reduction of ATP, average fluorescence density after irrigation, and growth after incubation was obtained in LAI group than in CNI group (p < .05).LAI can improve bacteriostasis effect of 2% NaOCl (p < .05). PIPS improved the antibacterial effect of the 2% NaOCl used in root canal therapy.

show abstract

“…Uses of 17 % EDTA activated by PIPS during the irrigation protocol and after mechanical instrumentation resulted in better cleaning and smear layer removal when compared to other irrigation techniques [105,106]. Further studies have supported these findings and confirmed that the best results were obtained when PIPS used in combination with 17% EDTA [107][108][109][110].…”

Section: Using Laser To Remove Smear Layermentioning

confidence: 95%

Smear Layer in Endodontics: A review of its role and management

Abognah

2020

KJDMR

View full text Add to dashboard Cite

Whatever the technique used for root canal instrumentation; a layer of debris termed “smear layer” is formed. This is a layer composed of an organic and inorganic substance and it harbors bacteria and their by-products. Removing the smear layer enhances the cleaning and disinfecting of root canal walls and grant maximum adaptation of root canal filling materials. Nonetheless, the existence of smear layer can seal the dentinal tubules and decrease the probability of dentinal tubule’s invasion by bacteria and their toxins, this issue is still controversial. Smear layer removal approaches including chemical, ultrasonics, and laser have been widely studied. However, none of them is entirely efficient along the length of all canals or is universally recognized. If the decision was to remove smear layer, irrigating root canals with EDTA followed by NaOCl will fulfill the objective. It is still debatable whether the removal of smear layer will decrease the apical leakage or not. Additional research is required to determine the consequence of removing or preserving the smear layer.

show abstract

Antibacterial and Smear Layer Removal Efficacy of Er:YAG Laser Irradiation by Photon-Induced Photoacoustic Streaming in Primary Molar Root Canals: A Preliminary Study

Abstract: These findings suggest that Er:YAG laser by PIPS-activated irrigation can be considered as an effective irrigant agitation technique in pediatric endodontics.

Cited by 34 publications

References 40 publications

Influence of Photon-Induced Photoacoustic Streaming (PIPS) on Root Canal Disinfection and Post-Operative Pain: A Randomized Clinical Trial

Influence of Photon-Induced Photoacoustic Streaming (PIPS) on Root Canal Disinfection and Post-Operative Pain: A Randomized Clinical Trial

Efficacy of the photon‐initiated photoacoustic streaming combined with different solutions on Enterococcus faecalis in the root canals

Smear Layer in Endodontics: A review of its role and management

Contact Info

Product

Resources

About