Antibacterial Capacity and Identification of Bioactive Compounds by GCMS of Allium Cepa

Sharma, Deepak; Rani, Reena; Chaturvedi, Monika; Yadav, Jaya Parkash

doi:10.22159/ijpps.2018v10i2.23698

Cited by 9 publications

(4 citation statements)

References 17 publications

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“…Momoh et al [5] study shows that n-Hexadecanoic acid has antibacterial, antifungal, and anti-inflammatory activities. Powered red onion extracted with six different organic solvents (methanol, acetone, chloroform, benzene, petroleum ether and ethyl acetate) were analyze for their chemical constituent using GC-MS. A total of 43 compounds were identified by GC-MS analysis and out of them dodecanoic acid was found common in all the extracts [22]. Yadav et al [22] study also shows that the extracts contained 9,12-Octadecadienoic acid (Z,Z)-, methyl ester, Hexadecanoic acid and Hexadecanoic acid, methyl ester which were found in our study and the extracts poses antibacterial activities.…”

Section: Discussionsupporting

confidence: 73%

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Nutritional, Phytochemicals, GC-MS and Antibacterial Activities of Aqueous Red Onion (Allium cepa) Extract against Staphylococcus aureus and Escherichia coli

Momoh

Manuwa

Ayinde

et al. 2023

IJTDH

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Background of Study: Onion (Allium cepa) plant has been used for multiple purposes both for modern and traditional medicine. The study evaluates the atomic absorption spectroscopy (AAS), Gas chromatography–mass spectrometry (GC-MS) and antibacterial activities of aqueous red onion (Allium cepa) and azithromycin solution against Staphylococcus aureus and Escherichia coli. Methodology: The AAS, GC-MS and phytochemical screening of the aqueous red onion (Allium cepa) extract were determined using standard procedures. Antibacterial activities were determined by agar well diffusion method. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations were determined using standard procedure. Results: Mineral analysis shows that the minerals that were found in the red onion are: Na, Mg, Fe, Ca, K, Al and Cu. Aqueous extract of Allium cepa shows the presence of secondary metabolites like: saponin, tannins, alkaloids, flavonoids, phenolic compounds, reducing sugar, steroids etc. 56 compounds were identified using GC-MS analysis with 9, 12-octadecadienoic acid (Z,Z)- being the most abundant with peak area of 50.50% and retention time of 16.563. The results revealed that aqueous A. cepa extract with concentration of 250 mg/ml showed strong response against Staphylococcus aureus and Escherichia coli with zone of inhibition of 22.67±1.585 and 28.18±1.689 respectively. At 100 mg/ml, the onion extract exhibited weak and moderate response against both organisms. The azithromycin solution has zone of inhibition values that ranges from 9.25 ± 0.73 to 20.03 ± 1.16 mm for both organisms tested at 5.00 and 20.00 mg/mL respectively. The MIC values of the azithromycin solution and aqueous red onion extract for S. aureus and E. coli were 31.25, 31.25, 500 and 250 mg/mL while their MBC values were 62.50, 62.50, 1000.00 and 500.00 mg/ml respectively. MBC/MIC values indicate that azithromycin solution and aqueous red onion extract had bactericidal effects on both organisms tested. The red onion has potential as natural therapeutic agents and may prevent pathogenic diseases caused by Staphylococcus aureus and Escherichia coli.

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Section: Discussionsupporting

confidence: 73%

“…Bacillus subtilis were resistant while the most sensitive was Candida albicans [23]. "Allium cepa methanol extract shows good antibacterial activity against different bacterial strains and the methanol extract was found most potent against K. pneumonia and S. marcescens with the zone of inhibition of 26±0.76 mm for both strains" [22].…”

Section: Discussionmentioning

confidence: 99%

Nutritional, Phytochemicals, GC-MS and Antibacterial Activities of Aqueous Red Onion (Allium cepa) Extract against Staphylococcus aureus and Escherichia coli

Momoh

Manuwa

Ayinde

et al. 2023

IJTDH

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“…They may be produced by the plant defense itself from stress as secondary metabolites. These phytoprotectants proved to possess pharmacological activity acts in similar away as synthetic drugs [12]. The palmitic acid reported possessing anticancer activity, antimicrobial, and nematicide activity.…”

Section: Discussionmentioning

confidence: 99%

Identification of Bioactive Compounds in Cymodocea Serrulata-a Seagrass by Gas Chromatography–mass Spectroscopy

Pushpabharathi

Jayalakshmi

Amudha

et al. 2018

Asian J Pharm Clin Res

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Objective: The objective of this study was to identify the lead phytocompounds present in the ethanol extract of the seagrass Cymodocea serrulata by gas chromatography–mass spectrometry (GCMS).Methods: 1 kg of C. serrulata whole seagrass powder was subjected to extraction on polarity basis using five solvent such as hexane, chloroform, ethyl acetate, ethanol, and water. Since ethanol extract showed a maximum antioxidant property, its phytochemicals were investigated using GCMS technique. The phytocompounds identified through GC were interpreted with mass spectra national institute standard and technology library.Result: The GCMS analysis of ethanol extract of C. serrulata identified peaks of six different compounds they are hexahydrofarnesyl acetone (7.70%), hexadecanoic acid, methyl ester (4.11%), tetradecanoic acid (62.89%), pentadecanoic acid(62.89%), cholesta4, 6dien3ol (5.88%), and stigmasterol (19.42%).Conclusion: The GCMS study of C. serrulata ethanol extract unveils the presence of bioactive compounds that have a pharmacological and nutraceutical values.

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“…A total of 200 µl of test sample was loaded into each well, with a concentration of 10 mg/well. The plates were incubated at 37°C overnight, and diameter of the zone of inhibition was measured [19][20][21].…”

Section: Agar Well Diffusionmentioning

confidence: 99%

Anti-Methicillin Resistant Staphylococcus Aureus Potential of Phytochemicals in Terminalia Catappa and Their Proposed in Silico Mechanism of Action

RAVI¹,

JINDAM²,

KUMARESAN³

et al. 2019

Asian J Pharm Clin Res

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Objective: The objective of this study was to investigate the antibacterial potential of leaves of this Terminalia catappa and identify the mechanism of action for those phytochemicals present in this leaves. Methods: Phytochemicals were extracted using maceration and the extracts were analyzed using gas chromatography–mass spectrometry (GC-MS) to identify the chemical structure. Antibacterial potential was evaluated using agar well diffusion. The phytochemicals were subjected to in silico protein–ligand docking study to identify the mechanism of action. Results: In vitro antibacterial study demonstrated that the ethanol extract of the leaves has significant antibacterial activity against Staphylococcus aureus (SA) and methicillin-resistant SA (MRSA) with a zone of inhibition of 16 mm and 18 mm, respectively, at a concentration of 2 mg/ml. The chloroform and hexane extracts of the leaves did not demonstrate any significant activity. Based on GC-MS analysis and literature review, 12 phytochemicals were identified to be present in the ethanol extract of the T. catappa leaves. These molecules were subjected to in silico protein–ligand docking study against common drug target proteins of SA and MRSA. Among the studied ligands, granatin A demonstrated the highest significance to inhibit topoisomerase IV with a binding energy of −11.3 kcal/mol and produced 7 hydrogen bonds, followed by punicalin with −10.7 kcal/mol binding energy toward penicillin-binding protein 2a with 6 hydrogen bonds. Conclusion: Phytochemicals of T. catappa demonstrates significant drug ability potential against drug-resistant MRSA pathogen and demands further investigation on their individual activity and mechanism.

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Antibacterial Capacity and Identification of Bioactive Compounds by GCMS of Allium Cepa

Cited by 9 publications

References 17 publications

Nutritional, Phytochemicals, GC-MS and Antibacterial Activities of Aqueous Red Onion (Allium cepa) Extract against Staphylococcus aureus and Escherichia coli

Nutritional, Phytochemicals, GC-MS and Antibacterial Activities of Aqueous Red Onion (Allium cepa) Extract against Staphylococcus aureus and Escherichia coli

Identification of Bioactive Compounds in Cymodocea Serrulata-a Seagrass by Gas Chromatography–mass Spectroscopy

Anti-Methicillin Resistant Staphylococcus Aureus Potential of Phytochemicals in Terminalia Catappa and Their Proposed in Silico Mechanism of Action

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