Antibacterial, cytotoxic and phytochemical screening of some traditional medicinal plants in Brazil

Bouzada, Maria Lúcia M.; Fabri, Rodrigo Luiz; Nogueira, Mauro S.; Konno, Tatiana Ungaretti Paleo; Duarte, Gizele G.; Scio, Elita

doi:10.1080/13880200802411771

Cited by 50 publications

(34 citation statements)

References 38 publications

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“…It is also reported, as well as in our research, the antibacterial activity of this essential oil against E. cloacae and E. aerogenes (28). Bouzada et al (29) verified any antibacterial activity of the E. uniflora methanolic extract against S. typhimurium, and Barbosa et al (30) registered in her research, the need for high concentrations of Brazillian cherry essential oil for inhibition of clinical and food isolates of Salmonella enteric typhimurium and S. enteric Enteretidis, respectively, unlike the data found in our study.…”

Section: Resultscontrasting

confidence: 56%

BIOLOGICAL PROPERTIES OF Eugenia uniflora L. ESSENTIAL OIL: CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY

Becker

Volcão²,

Camargo³

et al. 2017

VITTALLE, ISSN 1413-3563, Rio Grande, Brasil

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Section: Resultscontrasting

confidence: 56%

BIOLOGICAL PROPERTIES OF Eugenia uniflora L. ESSENTIAL OIL: CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY

Becker

Volcão²,

Camargo³

et al. 2017

VITTALLE, ISSN 1413-3563, Rio Grande, Brasil

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“…También, Bouzada et al (2009) obtuvieron que los extractos más citotóxicos fueron los que mostraron mayor contenido de alcaloides. JohnsonSenjobi, Moody y Ettu (2011) observaron que los alcaloides extraídos en el extracto alcohóli-co de C. aconitifolius también presentaron alta toxicidad (CL 50 44.7 μg/mL).…”

Section: Discussionunclassified

Concentraciones de alcaloides, glucósidos cianogénicos, polifenoles y saponinas en plantas medicinales seleccionadas en Ecuador y su relación con la toxicidad aguda contra Artemia salina

Jaramillo-Jaramillo

Jaramillo-Espinoza

D’Armas

et al. 2016

RBT

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Concentraciones de alcaloides, glucósidos cianogénicos, polifenolesy saponinas en plantas medicinales seleccionadas en Ecuador y su relación con la toxicidad aguda contra Artemia salina Alcoholic and aqueous extracts from leaves of these selected plants were prepared at different concentrations. To assess cytotoxicity of these extracts, different bioassays with A. salina were undertaken, and the mortality rates and LC 50 were obtained. Besides, concentrations of alkaloids, cyanogenic glycosides, phenols, tannins and saponins were determined by spectrophotometric methods; this constituted the first report of quantification of secondary metabolites in the selected plants from Ecuador. T. officinale had the highest concentration of total phenols (22.30 ± 0.23 mg/g) and tannins (11.70 ± 0.10 mg/g), C. aconitifolius of cyanogenic glycosides (5.02 ± 0.37 µg/g) and P. hysterophorus of saponins (6.12 ± 0.02 mg/g). Tannins values obtained were not adverse to their consumption. Alcoholic and aqueous extracts of selected plants had hemolytic activity depending on the concentration of saponins. Although the values of cyanogenic glycosides were permissible, it was necessary to monitor the presence of this metabolite in plants to minimize health problems. LC 50 values ranged from extremely toxic (3.37 µg/mL) to highly toxic (274.34 μg/mL), in P. carpunya and T. officinale, respectively. From correlation analysis, it was observed that increase values of alkaloids concentrations had highly significant (p<0.001) acute toxicity against A. salina, while at a higher polyphenol concentration the level of plants cytotoxicity decreased significantly (p<0.001). The results of principal component analysis showed that saponins apparently were in synergy with polyphenols to decrease cytotoxicity, but antagonize with alkaloids and cyanogenic glycosides, indicating that these secondary metabolites present variability in the mechanisms of action against A. salina, as cytotoxic compounds. These results also demonstrate that polyphenols and saponins can be lethal at low concentrations, demonstrating the potential of brine shrimp bioassay as a model to evaluate plant extracts containing low concentrations of chemical compounds with high polarities. The significant positive correlation between cytotoxicity and concentration of alkaloids confirmed by the bioassay of brine shrimp can be useful to identify promising sources of antitumor compounds, and to evaluate tolerable limits not affecting other benign cells. Contents of secondary metabolites found in the selected plants confer them great pharmacologic values. Rev. Biol. Trop. 64 (3):

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“…Satoh and his co-workers [14] has shown that some ingredients of L. sibiricus like furanoditerpenelactones and the diterpene-lactones, leonotinin, leonotin, dubiin and nepetaefuran exhibited moderate cytotoxic activity with IC 50 value of 50-60 µg/mL against leukemia cells (L 1210). Cytotoxic activities of methanolic extract of aerial parts of this plant have been reported previously by Maria et al [30]. Cruz and his co-workers [15] found that the dichloromethane extracts from the aerial parts of L. sibiricus L killed Artemia salina with LC 50 of 12 ppm.…”

Section: Resultsmentioning

confidence: 91%

In Vitro Cytotoxic and Anthelmintic Activities of <i>Leonurus sibiricus L.</i>

et al. 2012

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This study investigated the cytotoxic and anthelmintic activities of Leonurus sibiricus L.(commonly known as Raktodrone in Bangladesh) belonging to the family Labiatae. The dried leaves and roots of L. sibiricus were extracted with methanol and fractionated by modified Kupchan method. The crude methanolic extracts as well as its soluble fractions of petroleum ether, ethyl acetate and chloroform were screened for cytotoxic activity using brine shrimp lethality bioassay. They were found to possess significant cytotoxic activities. The LC 50 values of crude extract of leaves and its pet-ether, ethyl acetate and chloroform soluble fractions were 1.0, 2.0, 2.11 and1.33 µg/ml, respectively. On the other hand, the LC 50 of crude methanolic extract of roots and fractions of pet-ether, ethyl acetate and chloroform were 2.0µg/ml, 2.81 µg/ml, 3.55 µg/ml and 7.58µg/ml, respectively. Vincristine sulphate was used as positive control. The crude methanol extract of leaves and roots also showed very good anthelmintic activities as determined against the earthworms, Pheretima posthuma. The study confirms the moderate anthelmintic and potent cytotoxic activities of leaf and root extracts of L. sibiricus.

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Antibacterial, cytotoxic and phytochemical screening of some traditional medicinal plants in Brazil

Cited by 50 publications

References 38 publications

BIOLOGICAL PROPERTIES OF Eugenia uniflora L. ESSENTIAL OIL: CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY

BIOLOGICAL PROPERTIES OF Eugenia uniflora L. ESSENTIAL OIL: CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY

Concentraciones de alcaloides, glucósidos cianogénicos, polifenoles y saponinas en plantas medicinales seleccionadas en Ecuador y su relación con la toxicidad aguda contra Artemia salina

In Vitro Cytotoxic and Anthelmintic Activities of <i>Leonurus sibiricus L.</i>

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