Antibacterial Electrospun Poly(lactic acid) (PLA) Nanofibrous Webs Incorporating Triclosan/Cyclodextrin Inclusion Complexes

Abstract

Solid triclosan/cyclodextrin inclusion complexes (TR/CD-IC) were obtained and then incorporated in poly(lactic acid) (PLA) nanofibers via electrospinning. α-CD, β-CD, and γ-CD were tested for the formation of TR/CD-IC by a coprecipitation method; however, the findings indicated that α-CD could not form an inclusion complex with TR, whereas β-CD and γ-CD successfully formed TR/CD-IC crystals, and the molar ratio of TR to CD was found to be 1:1. The structural and thermal characteristics of TR/CD-IC were investigated by (1)H NMR, FTIR, XRD, DSC, and TGA studies. Then, the encapsulation of TR/β-CD-IC and TR/γ-CD-IC in PLA nanofibers was achieved. Electrospun PLA and PLA/TR nanofibers obtained for comparison were uniform, whereas the aggregates of TR/CD-IC crystals were present and distributed within the PLA fiber matrix as confirmed by SEM and XRD analyses. The antibacterial activity of these nanofibrous webs was investigated. The results indicated that PLA nanofibers incorporating TR/CD-IC showed better antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria compared to PLA nanofibers containing only TR without CD-IC. Electrospun nanofibrous webs incorporating TR/CD-IC may be applicable in active food packaging due to their very high surface area and nanoporous structure as well as efficient antibacterial property.

Quercetin/β-cyclodextrin inclusion complex embedded nanofibres: Slow release and high solubility

“…Incorporation of QU/b-CD-IC into electrospun nanofibres could be a valuable approach to use QU in a more efficient way, because of the high surface area-tovolume ratio and highly porous structure of nanofibres. Related studies on CD-IC incorporated electrospun nanofibres for different purposes have already been reported by our group (Aytac, Dogan, Tekinay, & Uyar, 2014;Aytac, Sen, Durgun, & Uyar, 2015;Canbolat, Celebioglu, & Uyar, 2014;Celebioglu, Umu, Tekinay, & Uyar, 2014;Kayaci, Ertas, & Uyar, 2013;Kayaci, Sen, Durgun, & Uyar, 2014;Kayaci, Umu, Tekinay, & Uyar, 2013;Kayaci & Uyar, 2012). CDs were also used for crosslinking of polymeric materials (Li & Hsieh, 2005;Liu, Zhang, Liu, Ni, & Li, 2013).…”

Release and antibacterial activity of allyl isothiocyanate/β-cyclodextrin complex encapsulated in electrospun nanofibers

“…Active agents loaded electrospun nanofibers may be useful in food packaging [3,4,[7][8][9][10][11] and biomedical applications [12][13][14] as reported in the literature. Vega-Lugo et al made comparison in terms of release characteristics of free AITC and AITC/␤-CD-IC including soy protein nanofibers with changing relative humidity and they also studied the effect of AITC amount on the release of AITC from AITC including polylactic acid nanofibers in different relative humidity [8].…”

“…[1,2]. Owing to the room temperature and ambient process conditions that provide protection for the bioactive compounds in electrospun nanofibers, electrospinning is used for production of functional nanofibers containing active agents like antioxidants [3,4], flavors/fragrances [5,6] and antibacterial agents [7].…”

“…In another related study, Ge et al produced materials for food packaging applications by immobilizing glucose oxidase enzyme in polyvinyl alcohol/chitosan/tea extract electrospun nanofibrous membrane [9]. In a study conducted by our research group, polylactic acid nanofibers incorporated with inclusion complex of triclosan and cyclodextrins were produced via electrospinning [7]. In a study of Pérez-Masiá et al, a phase changing material was encapsulated in electrospun zein fibers for the possible application as smart packaging materials [10].…”

Preparation and Characterization of Inclusion Complex between β-Cyclodextrin and Polylactic Acid

“…As illustrated in Figure 6, the typical diffraction peaks for pure PLA at 2θ=16.6, 19.1, 22.5, and 14.9 o were clearly observed while major peaks of β-CD appeared at 6.53, 9.3, 12.7 and 23.1 o , respectively which were consistent with the literatures. 33,34 It can be found that the XRD pattern of β-CD-PLA ICs is totally different from that of PLA and β-CD. Compared with pure PLA, with the content of the added β-CD increasing, the peaks of ICs were dramaticlly weaken and tightened.…”