Antibacterial Evaluation, Phytochemical Screening and Ascorbic Acid Assay of Turmeric (Curcuma longa)

Abstract

The ethanolic extract of Turmeric (Curcuma longa) was subjected to microbial susceptibility test using the agar well diffusion method. The extract was found to be active against the bacteria used as it inhibited all the organisms with the highest inhibition zone of 13.7mm recorded against Shigella flexnerri and the least inhibition of 2.3mm recorded against Staphylococcus epidermis. Minimum Inhibitory Concentration (MIC) values range from 6.25mg/ml to 200mg/ml. Shigella flexnerri gave MIC of 6.25mg/ml, S. aureus and K. Pneumonia, 25mg/ml, E. coli, 50mg/ml, S. epidermis, Lactobacillus and P. aeruginosa, 100mg/ml and V. cholera and S. typhi 200mg/ml respectively. Secondary metabolites were also screened using phytochemistry. Fourteen (14) secondary metabolites were screened from the ethanolic extract. The presence of these phytochemicals like tannins, alkaloids, phenols, steroids, flavonoids, phlobatannin, cardiac glycosides, terpenoids, triterpenes, saponin, etc; is indicative of the antimicrobial activity of Turmeric. The extract was found to contain 66.749mg/100ml ascorbic acid which is relatively comparable to many fruits recommended by dieticians. The value of curcumin present (Rf value 0.436) is also relatively close to that of pure curcumin. The results so far obtained are indicative of the good medicinal value of Turmeric in both pharmaceutical and pharmacological formulations.

Assessment of Phytochemical, Antioxidant and Antibacterial Activity of Balanites Aegyptiaca and Curcuma Longa against Some Bacterial Pathogens Isolated from Dairy Cow Infected with Mastitis

“…Phytochemical screening of the EE-CLP indicated the presence of flavonoids, steroids, saponins, alkaloids, phenolic compounds, terpenoids and tannins, these findings are in agreement with Oghenejobo et al (2017), who detected the same compounds in the EE-CLP. Also phytochemical screening of the C. longa powder confirmed the presence of carbohydrates, proteins and amino-acids, alkaloids, terpenoids and flavinoids but no tannins, glycosides and saponins (Pawar et al, 2014).…”

Phytochemicals, Antioxidants and Anti-tyrosinase Analyses of Selected Ginger Plants

“…18 While, ethanolic rhizome extract of turmeric (C. longa) had presented reducing sugars, alkaloids, coumarins, flavonoids, phenol, steroids and terpenoids at high concentration and the turmeric extract was found to affect against the bacteria that cause of diarrhoea (Shigella flexnerri, Staphylococcus aureus, S. epidermidis, Klebsiella pneumoniae, E. coli, P. aeruginosa, Lactobacillus, Vibrio cholerae, and S. typhi. 19 From previous research were suggested that turmeric have anti-inflammatory, anti-fungal, anti-tumor, against arthritis, antiwrinkles and antityriosinase. [20][21][22] Phytochemicals of methanolic leaves and stems extracts of Etlingera coccinea presented cardiac glycosides, steroids, saponins, anthraquinones, while rhizome of E. coccinea was found cardiac glycosides and steroids, leaves of E. coccinea had total antioxidant capacity, antibacterial, and antifungal higher than stem and rhizome parts.…”

Efek Sinergis Kombinasi Ekstrak Rimpang Kunyit (Curcuma domestica Val.) dan Ampisilin terhadap Escherichia coli secara in vitro

“…Dari uji efek antimikroba dan efek sinergis kombinasi ekstrak rimpang kunyit dan ampisilin metode agar diffusion didapatkan data seperti pada Tabel 1 sebagai berikut: berbagai tempat di Indonesia. [16][17] Penelitian ini adalah penelitian mengenai efek sinergis kombinasi ERK (Curcuma domestica Val.) dan ampisilin terhadap E. coli menggunakan konsentrasi ERK (Curcuma domestica Val.)…”

“…seperti polifenol kurkumin, flavonoid, alkaloid, tanin dan minyak atsiri dapat berperan sebagai agen antimikroba terhadap beberapa bakteri patogen termasuk E. coli. [14][15][16][17][18] Banyak penelitian yang meneliti tentang aktivitas antimikroba rimpang kunyit ini dengan berbagai konsentrasi dan dilaporkan mempu menghambat pertumbuhan E. coli secara in vitro. [14][15][16][17][18][19] Produk herbal yang dikombinasi dengan antibiotik sebagai terapi komplementer diharapkan mampu meningkatkan efek antimikroba satu sama lain (efek sinergis).…”

“…[14][15][16][17][18] Banyak penelitian yang meneliti tentang aktivitas antimikroba rimpang kunyit ini dengan berbagai konsentrasi dan dilaporkan mempu menghambat pertumbuhan E. coli secara in vitro. [14][15][16][17][18][19] Produk herbal yang dikombinasi dengan antibiotik sebagai terapi komplementer diharapkan mampu meningkatkan efek antimikroba satu sama lain (efek sinergis). 12,19 Penelitian mengenai efek sinergis dari kombinasi herbal dan antibiotik pada penelitian Azucena et al 12 pada tahun 2019 didapatkan bahwa zat kurkumin tidak memiliki efek antimikroba, tetapi kombinasi kurkumin murni dan ampisilin memiliki efek sinergis terhadap E. coli dengan menggunakan metode agar diffusion.…”