Antibegomoviral activity of the agrobacterial virulence protein VirE2

Sunitha, Sukumaran; Marian, Dolly; Höhn, Barbara; Veluthambi, K.

doi:10.1007/s11262-011-0654-0

Cited by 15 publications

(11 citation statements)

References 61 publications

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“…Thus, the Agrobacterium Ti plasmid virulence gene virE2 , encoding a nuclear ssDNA binding protein was expressed in N. benthamiana. The resulting transgenic plants were resistant to SLCMV (Resmi et al, 2015), in agreement with a report by Sunitha et al (2011) showing virE2 -mediated resistance to Mungbean yellow mosaic begomovirus (MYMV).…”

Section: Engineered Resistance To Cmgssupporting

confidence: 89%

The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead

Fondong

2017

Front. Plant Sci.

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The cassava mosaic disease (CMD), which occurs in all cassava growing regions of Africa and the Indian subcontinent, is caused by cassava mosaic geminiviruses (CMGs). CMGs are considered to be the most damaging vector-borne plant pathogens. So far, the most successful approach used to control these viruses has been the transfer of a polygenic recessive resistance locus, designated CMD1, from wild cassava to cassava cultivars. Further progress in harnessing natural resistance to contain CMGs has come from the discovery of the dominant monogenic resistance locus, CMD2, in some West African cassava cultivars. CMD2 has been combined with CMD1 through genetic crosses. Because of the limitations of the cassava breeding approach, especially with regard to time required to produce a variety and the loss of preferred agronomic attributes, efforts have been directed toward the deployment of genetic engineering approaches. Most of these approaches have been centered on RNA silencing strategies, developed mainly in the model plant Nicotiana benthamiana. Early RNA silencing platforms assessed for CMG resistance have been use of viral genes for co-suppression, antisense suppression or for hairpin RNAs-mediated gene silencing. Here, progress and challenges in the deployment of these approaches in the control of CMGs are discussed. Novel functional genomics approaches with potential to overcome some of the drawbacks of the current strategies are also discussed.

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Section: Engineered Resistance To Cmgssupporting

confidence: 89%

The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead

Fondong

2017

Front. Plant Sci.

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“…Thus, the Rep-binding peptide octamers may serve as an efficient strategy for engineering transgenic tomato plants that are resistant to diverse begomoviruses. Likewise, expression of the single-stranded DNA binding protein virE2 from Agrobacterium in tobacco has been shown to reduce Mungbean yellow mosaic virus DNA accumulation, although the spectrum of the resistance has not been established (Sunitha et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Sustained NIK‐mediated antiviral signalling confers broad‐spectrum tolerance to begomoviruses in cultivated plants

Brustolini¹,

Machado²,

Condori-Apfata³

et al. 2015

Plant Biotechnology Journal

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SummaryBegomovirus-associated epidemics currently threaten tomato production worldwide due to the emergence of highly pathogenic virus species and the proliferation of a whitefly B biotype vector that is adapted to tomato. To generate an efficient defence against begomovirus, we modulated the activity of the immune defence receptor nuclear shuttle protein (NSP)-interacting kinase (NIK) in tomato plants; NIK is a virulence target of the begomovirus NSP during infection. Mutation of T474 within the kinase activation loop promoted the constitutive activation of NIK-mediated defences, resulting in the down-regulation of translation-related genes and the suppression of global translation. Consistent with these findings, transgenic lines harbouring an activating mutation (T474D) were tolerant to the tomato-infecting begomoviruses ToYSV and ToSRV. This phenotype was associated with reduced loading of coat protein viral mRNA in actively translating polysomes, lower infection efficiency and reduced accumulation of viral DNA in systemic leaves. Our results also add some relevant insights into the mechanism underlying the NIK-mediated defence. We observed that the mock-inoculated T474D-overexpressing lines showed a constitutively infected wild-type transcriptome, indicating that the activation of the NIKmediated signalling pathway triggers a typical response to begomovirus infection. In addition, the gain-of-function mutant T474D could sustain an activated NIK-mediated antiviral response in the absence of the virus, further confirming that phosphorylation of Thr-474 is the crucial event that leads to the activation of the kinase.

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“…Sera [ 21 ] reported that expression of an artificial zinc finger protein targeting the replication origin of Beet severe curly top virus efficiently blocked viral DNA replication in transgenic Arabidopsis plants. Sunitha et al [ 22 ] expressed the A. tumefaciens Ti plasmid virE2 gene in tobacco and showed a reduction of viral DNA accumulation in leaf discs agroinoculated with Mungbean yellow mosaic virus (MYMV) partial dimers. Here, we report the efficacy of the virE2 gene in restricting SLCMV infection in transgenic Nicotiana benthamiana plants.…”

Section: Introductionmentioning

confidence: 99%

The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants

Resmi

Höhn

et al. 2015

Viruses

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Cassava mosaic disease is a major constraint to cassava cultivation worldwide. In India, the disease is caused by Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV). The Agrobacterium Ti plasmid virulence gene virE2, encoding a nuclear-localized, single-stranded DNA binding protein, was introduced into Nicotiana benthamiana to develop tolerance against SLCMV. Leaf discs of transgenic N. benthamiana plants, harboring the virE2 gene, complemented a virE2 mutation in A. tumefaciens and produced tumours. Three tested virE2 transgenic plants displayed reduction in disease symptoms upon agroinoculation with SLCMV DNA A and DNA B partial dimers. A pronounced reduction in viral DNA accumulation was observed in all three virE2 transgenic plants. Thus, virE2 is an effective candidate gene to develop tolerance against the cassava mosaic disease and possibly other DNA virus diseases.

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Antibegomoviral activity of the agrobacterial virulence protein VirE2

Cited by 15 publications

References 61 publications

The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead

The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead

Sustained NIK‐mediated antiviral signalling confers broad‐spectrum tolerance to begomoviruses in cultivated plants

The Agrobacterium tumefaciens Ti Plasmid Virulence Gene virE2 Reduces Sri Lankan Cassava Mosaic Virus Infection in Transgenic Nicotiana benthamiana Plants

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