Antibiofilm activity of flavonoid mangosteen pericarp extract against porphyromonas gingivalis bacteria

Abstract

<jats:p>Background. Necrosis teeth need a procedure called the root canal treatment to eliminate microorganisms in root canal, so the inflammation process did not continue to the periapical.One of the microbes that exist in thenecrosis teethis Porphyromonas gingivalis, that capable of forming biofilm because it has the ability to communicate with bacteria signaling (quorum sensing). It is necessary to discover another antibiofilm agent as an irrigation material in root canal preparation deriving from nature. Flavonoidmangosteen pericarp extract can disrupt acyl-homoserine lactones (AHLs) thus quorum sensing bacteria will be disrupted and causing permanent attachment and the establishment of colonies on the surface of the teeth any barrier. Purpose. The aim of this study found out the antibiofilmactivity of flavonoid mangosteen pericarp extract against Porphyromonas gingivalis bacteria. Method. This studyhasa laboratory experimental with post-test only group design. Porphyromonas gingivalis ATCC 33277 was diluted according to Mc. Farland standard 106CFU/ml in Tryptic Soy Broth (TSB) medium and inserted into microtitterplate flexible U bottom. Porphyromonas gingivalis was incubatedfor 6x24 hour and checked with a simple staining to see the formation of biofilm. Flavonoid mangosteen pericarp extract was added in various concentrations and OD (optical density) readings done with a wavelength of 595 nm. Result. Flavonoid mangosteen pericarp extractshowed a decrease of OD value from control in all concentrations.Conclusion.Flavonoid mangosteen pericarp extract has antibiofilm activity against Porphyromonas gingivalis bacteria.</jats:p>

The Antibacterial and Antibiofilm Effect of Amoxicillin and Mangifera indica L. Leaves Extract on Oral Pathogens

“…[ 26 ] In addition, flavonoids inhibit enzymes from producing quorum-sensing signals, thereby disrupting the communication process between cells during biofilm formation. [ 27 ] Steroids can cause leakage of lysosomes and membrane phospholipids that reduce the integrity of cell membranes and lead to cell lysis. [ 28 ]…”

Bioactivity Tracing of the Ethanol Extract of Bajakah Tampala (Spatholobus littoralis Hassk.) Typical Plant of Kalimantan Island as Antibiofilm of Staphylococcus aureus

“…In addition, research on the growth of the 48 h phase of biofilm is known to have a longer time compared to the 24 h phase, therefore, the biofilm communities formed in this phase are more numerous and organized with each other, thus forming a kind of stronger three-dimensional group that will communicate with each other so as to make the defense more difficult to penetrate [24]. This decrease in activity was also caused by the different amount of EPS between the middle phase and the maturation phase where the amount of EPS continued to increase according to the age of the biofilm [26].…”

“…In addition, the difference in concentration in this phase also plays a major role in providing inhibitory activity, according to Sonya's research [26]. In one study of the effectiveness of the extract on the biofilm formation of S. aureus bacteria, it was explained that a decrease in the average value of biofilm formation started at concentration and increased with increasing concentration [27].…”