Antibiotic delivery system using nano‐hydroxyapatite/chitosan bone cement consisting of berberine

Zou, Qin; Li, Yubao; Zhang, Li; Zuo, Yi; Li, Junfeng; Li, Jidong

doi:10.1002/jbm.a.32199

Cited by 43 publications

(16 citation statements)

References 53 publications

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“…Chitosani sal inear polymer composed of glucosamine (2amino-2-deoxy-d-glucopyranose), and is ac ationic polymer that is soluble in weakly acidic solution.C hitosan is ab iocompatible and biodegradable, pH-dependent cationicp olymer, which has become one of the most promising biopolymers for applicationsi nd rug delivery,t heranostics, gene therapy,a nd tissue engineering. [9] Combined with the advantageso fH AP, variousk inds of HAP/chitosan (HAP/CS) composite biomaterials, such as HAP/CS composite nanofibers, [10] scaffolds, [11] bone cements, [12] hydrogels, [13] and membranes, [14] have been synthesized and investigated for various applications.…”

Section: Introductionmentioning

confidence: 99%

Highly Flexible Multifunctional Biopaper Comprising Chitosan Reinforced by Ultralong Hydroxyapatite Nanowires

Sun

Chen

2017

Chemistry A European J

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Highly flexible multifunctional biopaper comprising ultralong hydroxyapatite nanowires and chitosan (UHANWs/CS), with high weight fractions of ultralong hydroxyapatite nanowires (UHANWs) up to 100 wt. %, is reported. The as-prepared UHANWs/CS composite biopaper has high flexibility and superior mechanical properties even when the weight fraction of UHANWs is as high as 90 wt. %. In contrast, the control samples consisting of hydroxyapatite nanorods and chitosan (HANRs/CS) with weight fractions of HANRs higher than 66.7 wt.% cannot be obtained in the form of the flexible membrane. The ultimate tensile strength and Young's modulus of the UHANWs/CS composite biopaper are about 3.2 times and 4.3 times those of the HANRs/CS membrane with the same weight fraction of HAP, respectively. In addition, the UHANWs/CS composite biopaper (90 wt. % UHANWs) can be used for color printing using a commercial ink-jet printer. The surface wettability, swelling ratio, and water vapor transmission rate of the UHANWs/CS composite biopaper are adjustable by changing the addition amount of UHANWs. In vitro experiments indicate that the UHANWs/CS composite biopaper has good degradability, high acellular bioactivity and high biocompatibility. The as-prepared UHANWs/CS composite biopaper is therefore promising for various biomedical applications such as wound dressing, bone-fracture fixation, and bone-defect repair.

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Section: Introductionmentioning

confidence: 99%

Highly Flexible Multifunctional Biopaper Comprising Chitosan Reinforced by Ultralong Hydroxyapatite Nanowires

Sun

Chen

2017

Chemistry A European J

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“…In the past few decades, moist exposed burn ointment (MEBO), a Chinese burn ointment with a USA patented formulation since 1995, which was developed at the China National Science and Technology Centre in Beijing in 1989 (23,24), has been used to treat thickness burns in clinical practice and has achieved beneficial efficacy (25)(26)(27)(28) (30) and that berberine exhibits antimicrobial effects (31). Clinical and experimental studies have shown that MEBO exhibits analgesic and antimicrobial effects, and reduces the treatment time of burns (25)(26)(27)(28)32,33).…”

Section: Introductionmentioning

confidence: 99%

Moist exposed burn ointment promotes cutaneous excisional wound healing in rats involving VEGF and bFGF

Tang

Han

Feng

et al. 2014

Molecular Medicine Reports

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Abstract. Cutaneous delayed wounds are a challenging clinical problem, and vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) exhibit key roles in wound healing. Moist exposed burn ointment (MEBO), a Chinese burn ointment with a USA patented formulation, has been reported to promote chronic ischemic and neurogenic ulcer healing in patients; however, the underlying mechanisms remain unclear. In the present study, MEBO significantly promoted the formation of granulation tissue in cutaneous excisional wounds, shortened the time of wound healing, and increased neovascularization and the number of fibroblasts. Furthermore, as well as enhancing the protein expression, MEBO application also increased the gene expression of VEGF and bFGF. The results indicate that MEBO promotes cutaneous excisional wound healing by at least partially enhancing VEGF and bFGF production, implicating the potential uses of MEBO for delayed cutaneous wound healing. IntroductionCutaneous wounds, known as ulcers, are an extremely common clinical problem and often arise following acute or chronic mechanical causes, physical or chemical burns, frostbite, infections, and disorders, including rheumatism, diabetes, peripheral vascular disease, lipodermatosclerosis and malignant tumors (1,2). Furthermore, cutaneous wound healing may be significantly delayed due to the aforementioned factors. At present, delayed cutaneous wounds are one of major burdens for health care, and lead to a reduced quality of life in patients who suffer from this type of wound (3,4).Wound healing is a complicated biological process involving a series of dynamic events, including hemostasia, inflammation, cell proliferation and differentiation, neovascularization, granulation tissue formation, collagen synthesis, epithelialization, and wound contraction. Increasing evidence has established the hypothesis that growth factors, including vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), are key regulators of normal and abnormal angiogenesis, and tissue repair in animals and humans (5-8). VEGF promotes angiogenesis/vasculogenesis and vascular permeability, and enhances endothelial cell proliferation and migration as well as the adhesion of leukocytes (5,9). Further research revealed that VEGF stimulates hydrogen sulfide synthesis and release from endothelial cells, thus leading to subsequent endothelial cell growth, migration and permeability, microvessel formation, and wound healing (10). In addition, VEGF promotes epithelialization and collagen deposition in the wound (11). FGF-2, known as bFGF, is a member of a large FGF family and induces angiogenesis, endothelial cell and fibroblast proliferation, and wound healing (12)(13)(14). Recent data indicated that bFGF-mediated angiogenesis refers to endothelial cell proliferation, migration and tube formation by activating c-Jun N-terminal kinase/stress-activated protein kinase signaling (15). Notably, the expression of VEGF and bFGF was increased following skin in...

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“…The release kinetics of 27% HACC from the nanotubes was determined by the anthrone sulfuric acid reaction [29,34,35]. Three discs of NT-H with different diameters were each individually immersed in 1 ml of PBS in a 48-well plate (Costar3548, Corning Incorporated, Corning, NY, USA) at a temperature of 37 °C and agitated at 100 rpm.…”

Section: Methodsmentioning

confidence: 99%

Inhibited Bacterial Adhesion and Biofilm Formation on Quaternized Chitosan-Loaded Titania Nanotubes with Various Diameters

et al. 2016

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Titania nanotube-based local drug delivery is an attractive strategy for combating implant-associated infection. In our previous study, we demonstrated that the gentamicin-loaded nanotubes could dramatically inhibit bacterial adhesion and biofilm formation on implant surfaces. Considering the overuse of antibiotics may lead to the evolution of antibiotic-resistant bacteria, we synthesized a new quaternized chitosan derivative (hydroxypropyltrimethyl ammonium chloride chitosan, HACC) with a 27% degree of substitution (DS; referred to as 27% HACC) that had a strong antibacterial activity and simultaneously good biocompatibility with osteogenic cells. Titania nanotubes with various diameters (80, 120, 160, and 200 nm) and 200 nm length were loaded with 2 mg of HACC using a lyophilization method and vacuum drying. Two standard strain, methicillin-resistant Staphylococcus aureus (American Type Culture Collection 43300) and Staphylococcus epidermidis (American Type Culture Collection 35984), and two clinical isolates, S. aureus 376 and S. epidermidis 389, were selected to investigate the bacterial adhesion at 6 h and biofilm formation at 24, 48, and 72 h on the HACC-loaded nanotubes (NT-H) using the spread plate method, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM). Smooth titanium (Smooth Ti) was also investigated and compared. We found that NT-H could significantly inhibit bacterial adhesion and biofilm formation on its surface compared with Smooth Ti, and the NT-H with 160 nm and 200 nm diameters had stronger antibacterial activity because of the extended HACC release time of NT-H with larger diameters. Therefore, NT-H can significantly improve the antibacterial ability of orthopedic implants and provide a promising strategy to prevent implant-associated infections.

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Antibiotic delivery system using nano‐hydroxyapatite/chitosan bone cement consisting of berberine

Cited by 43 publications

References 53 publications

Highly Flexible Multifunctional Biopaper Comprising Chitosan Reinforced by Ultralong Hydroxyapatite Nanowires

Highly Flexible Multifunctional Biopaper Comprising Chitosan Reinforced by Ultralong Hydroxyapatite Nanowires

Moist exposed burn ointment promotes cutaneous excisional wound healing in rats involving VEGF and bFGF

Inhibited Bacterial Adhesion and Biofilm Formation on Quaternized Chitosan-Loaded Titania Nanotubes with Various Diameters

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