Antibiotic pollution in the Katari subcatchment of the Titicaca Lake: Major transformation products and occurrence of resistance genes

Archundia, D.; Duwig, Céline; Lehembre, Frédéric; Chirón, Serge; Morel, Marie‐Christine; Prado, Blanca; Bourdat-Deschamps, Marjolaine; Vince, Erwann; Aviles, G. Flores; Martins, Jean M.F.

doi:10.1016/j.scitotenv.2016.10.129

Cited by 75 publications

(36 citation statements)

References 75 publications

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“…The ARGs sequences inserted included resistance to tetracycline ( tetA, tetB ), β-lactam antibiotics ( bla OXA-2 , bla IMP-2 , bla IMP-5 , bla IMP-12 , acc-3 ), methicillin ( mecA ), quinolones ( qnrB1, qnrB5 and qnrS1 ), and macrolide-lincosamide-streptogramin B ( msrA ) sulfamethoxazole ( sul1 and sul2 ). The latter two were not included in the screening arrays but were previously reported in the area (32). In addition the sequences of the bacteriophage crAssphage and Class 1 integron ( intl1 ), were inserted in the same plasmid.…”

Section: Resultsmentioning

confidence: 99%

“…Previously, another study reported the presence of sul1 and sul2 in the Milluni valley, specifically in Pata Khota. When they analyzed the levels of sulfamethoxazole in water, the antibiotic was not detected (32) suggesting that the presence of these genes can occur naturally in bacteria residing in these aquatic environments, as has been found in other pristine sites (42-44). Taking into consideration that PK and MC have little antropogenic activity around, and that antibiotic levels are reported undetectable in one of our study sites (32) we assume antibiotics will not play a major rol in our analysis.…”

Section: Discussionmentioning

confidence: 96%

“…When they analyzed the levels of sulfamethoxazole in water, the antibiotic was not detected (32) suggesting that the presence of these genes can occur naturally in bacteria residing in these aquatic environments, as has been found in other pristine sites (42-44). Taking into consideration that PK and MC have little antropogenic activity around, and that antibiotic levels are reported undetectable in one of our study sites (32) we assume antibiotics will not play a major rol in our analysis. Although we did not measured antibiotic levels inour sampled sites, erythromycin levels were reported under the limit of detection or in the order of ng/g of sediment for UP1 and UP2 nearby sites, respectively (Guzman-Otazo et al .…”

Section: Discussionmentioning

confidence: 96%

“…Twelve positive ARGs (CT < 39) were selected to perform the assays of absolute quantification. Additionally, sulfonamides resistance genes ( Sul1 and Sul2 ) were included in the analysis as previous reports point at their presence in Milluni (32).…”

Section: Methodsmentioning

confidence: 99%

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Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Agramont

Gutiérrez-Cortez

Joffré

et al. 2020

Preprint

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26Water and sediment samples affected by mining activities were collected from 27 three lakes in Bolivia, the pristine Andean lake Pata Khota, the Milluni Chico lake 28 directly impacted by acid mine drainage, and the Uru-Uru lake located close to 29 Oruro city and highly polluted by mining activities and human wastewater 30 discharges. Physicochemical parameters, including metal compositions, were 31 analyzed in water and sediment samples. Antibiotic resistance genes (ARGs), 32 were screened for, and verified by quantitative PCR together with the mobile 33 element class 1 integron (intl1) as well as crAssphage, a marker of human fecal 34 pollution. The gene intl1 showed a positive correlation with sul1, sul2, tetA and 35 blaOXA-2. CrAssphage was only detected in Uru-Uru lake and its tributaries and 36 significantly higher abundance of ARGs were found in these sites. Multivariate 37 analysis showed that crAssphage abundance, electrical conductivity and pH were 38 positively correlated with higher levels of intl1 and ARGs. Taken together our 39 results suggest that fecal pollution is the major driver of higher ARGs and intl1 in 40 wastewater and mining contaminated environments. 41 3 48 can be incorporated and replicated in environmental microorganisms, thereby 49 increasing their concentration (3). 50 It has been reported that anthropogenic activities cause pollution of aquatic 51 environments with ARGs and MGEs (4). Wastewater discharges cause co-52 occurrence of MGEs and different ARGs in water and sediments (5). At a 53 continental scale, ARGs in sediments are strongly correlated with MGEs and 54antibiotic residues (6). Recently, it has been observed that microorganisms living in 55 aquatic microbial communities that came from wastewater were able to transfer 56 ARGs via horizontal gene transfer (HGT) after exposure to low levels of antibiotics 57 and biocides (7). Many of the ARGs that can be found in clinical settings have also 58 been found in the environment, suggesting the possibility of movement and 59 dissemination between these two scenarios (8). 60Mining activities cause contamination of downstream water with dissolved 61 metals (9) where heavy metals tend to accumulate in sediments (10). It has been 62 suggested that heavy metals can favor selection of ARGs via co-selection, i.e. the 63 simultaneous acquisition of both, ARG and metal resistance genes, where the 64 presence of metals constitutes the selective pressure (11). Several studies support 65 this relation. Urban soil samples of Belfast in Northern Ireland, exhibited a pattern 66 of co-occurrence between metals (Zn, Cu, Cd, Co, Ni, Hg, Cr and As) and many 67 ARGs. Moreover, the degree of metal toxicity was positively correlated with the 68 abundance of MGEs, and ARGs (12). Metals, as Cu and Zncan in some cases 69 exert stronger selection pressure over soil microbial communities for the selection 70 of resistant bacteria, even more than specific antibiotics (13). In the Dongying river 71 in China, the levels of Cu and Cr were positively c...

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Agramont

Gutiérrez-Cortez

Joffré

et al. 2020

Preprint

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“…Several studies have already confirmed the environmental contamination, in many countries, by antibiotics, one example being the tetracycline contamination in Brazil, Canada, and China, (PINHEIRO et al, 2013;PULICHARLA et al, 2017;WEI et al, 2016). Other cases are: contamination by sulfonamine in Bolivia, the Czech Republic, and the United States of America (ARCHUNDIA et al, 2017;HO et al, 2013;KOBA et al, 2016); contamination by macrolide in South Korea, United Kingdom, and Hong Kong (KIM et al, 2015;PETRIE et al, 2016;WU et al, 2016); by fluoroquinolones in Poland, Austria, and Thailand (GBYLIK-SIKORSKA et al, 2015;MARTÍNEZ-CARBALLO et al, 2007;RICO et al, 2014); and by quinolones in China and Malaysia (HO et al, 2013;.…”

Section: Introductionmentioning

confidence: 95%

Compostagem de efluente suíno no tratamento de resíduos de fármacos veterinários

Bohrer

Carissimi

López

et al. 2019

SCA

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The increasing need for animal protein has exerted pressures on the current animal production system. One of the alternatives found by producers to improve animal performance has been the use of veterinary drugs, especially antibiotics. However, its indiscriminate use can be a risk to the environmental balance of the producing locations since Brazil has approximately 42 million pigs. With this herd, pig farming represents a great potential for generating environmental impacts. As an alternative use of the large volume of liquid pig manure generated, many producers have been using this slurry in the soil as a bio fertilizer. Residues of veterinary drugs are capable of accumulation with environmental matrices and leach into water resources. In this sense, it is mandatory to dedicate more efforts on the study of techniques and processes for the treatment of organic effluents contaminated by veterinary drugs. Low-cost and environmentally friendly alternative treatment systems are necessary, in order to minimize the entry of these contaminants into the environment. Therefore, the composting process, defined as aerobic microbial decomposition process of organic matter, can be an alternative for treating the effluents contaminated by veterinary drugs. This review to aims to create awareness in the academic community regarding the veterinary drug residues and their contamination potential in different environmental matrices, as well as evaluating the composting process as a technique to minimize the impacts of the swine activity waste on the environment.

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Antibiotic Pollution and Occurrence of Bacterial Antibiotic Resistance Genes in Latin American Developing Countries

Archundia

Duwig

Martins

et al. 2017

Antimicrobial Resistance in Wastewater Treatment Processes

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Antibiotic pollution in the Katari subcatchment of the Titicaca Lake: Major transformation products and occurrence of resistance genes

Cited by 75 publications

References 75 publications

Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Antibiotic resistance genes and class 1 integron: Evidence of fecal pollution as a major driver for their abundance in water and sediments impacted by metal contamination and wastewater in the Andean region of Bolivia

Compostagem de efluente suíno no tratamento de resíduos de fármacos veterinários

Antibiotic Pollution and Occurrence of Bacterial Antibiotic Resistance Genes in Latin American Developing Countries

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