Antibiotic Resistance Determinant-Focused Acinetobacter baumannii Vaccine Designed Using Reverse Vaccinology

Ni, Zhaohui; Chen, Yan; Ong, Edison; He, Yongqun

doi:10.3390/ijms18020458

Cited by 63 publications

(42 citation statements)

References 89 publications

(139 reference statements)

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“…Maximum penetration of the bacterial cell wall by NS has been observed due to opposite charges of attraction. These unique properties of multimode of actions and maximum invasion are quite helpful in developing NS as broad-spectrum antibiotics in the future ( Maqbool et al, 2017 ; Ni et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: CuO and CeO2 Nanostructures Green Synthesized Using Olive Leaf Extract Inhibits the Growth of Highly Virulent Multidrug Resistant Bacteria

et al. 2018

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One of the major challenges of nano-biotechnology is to engineer potent antimicrobial nanostructures (NS) with high biocompatibility. Keeping this in view, we have performed aqueous olive leaf extract mediated one pot facile synthesis of CuO-NS and CeO2-NS. Prepared NS were homogenous, less than 26 nm in size, and small crystallite units as revealed by scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses. Fourier transform infrared spectroscopy (FTIR) of CuO-NS and CeO2-NS showed typical Cu-O prints around 592–660 cm-1 and Ce-O bond vibrations at 453 cm-1. The successful capping of CuO-NS and CeO2-NS by compounds present in the plant extract was further validated by high performance liquid chromatography (HPLC) and thermal gravimetric analysis (TGA). Active phyto-chemicals from the leaf extract simultaneously acted as strong reducing as well as capping agent in the NS synthesis. NS engineered in the present study showed antibacterial potential at extremely low concentration against highly virulent multidrug-resistant (MDR) gram-negative strains (Escherichia coli, Enterobacter cloacae, Acinetobacter baumannii and Pseudomonas aeruginosa), alarmed by World Health Organization (WHO). Furthermore, CuO-NS and CeO2-NS did not show any cytotoxicity on HEK-293 cell lines and Brine shrimp larvae indicating that the NS green synthesized in the present study are biocompatible.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: CuO and CeO2 Nanostructures Green Synthesized Using Olive Leaf Extract Inhibits the Growth of Highly Virulent Multidrug Resistant Bacteria

et al. 2018

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“…WP_000413999, a siderophore receptor from cluster I, is strongly expressed in the early stages of bacteremia [26]. Moreover, Ni and collogues [48] reported this protein as a vaccine candidate using reverse vaccinology. In this study, we made an attempt to determine the physicochemical characteristics, 3D structure, and immunogenic properties of WP_000413999 followed by their evaluation in the experimental animal model.…”

Section: Discussionmentioning

confidence: 99%

Protective potentials of a siderophore receptor against Acinetobacter baumannii infections

2019

Biointerface Res Appl Chem

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Acinetobacter baumannii causes nosocomial infections and high mortality rates in the world. Since antibiotic treatment is complicated by extensive drug resistance in A. baumannii strains, other approaches such as vaccination can be a cost-effective solution. Siderophore receptor related to cluster 1 of iron uptake system (WP_000413999) is expressed in early stages of A. baumannii’s infection under iron restricted conditions. In this study, structural characterization and immunogenicity of the target protein in a murine sepsis model were assessed. Structural properties of the siderophore receptor were determined by bioinformatics tools. The gene encoding the antigen was cloned in E. coli BL21(DE3) and was then expressed. The purified recombinant protein was administered to the mice subcutaneously for antibody production. The immunized mice were challenged with the A. baumannii at lethal dose via intraperitoneal injection. Blast results revealed more than 97% identity of the protein in 3472 strains of A. baumannii. This receptor is a TonB-dependent transporter with a barrel domain composed of 22 β-strands connected by external loops and periplasmic turns. Experimental findings showed that the recombinant protein had no toxicity effect on A549 cells. Moreover, the studied protein was able to induce a specific antibody response in the mice. Survival rate of the passive immunized mice was improved against sepsis caused by A. baumannii ATCC 19606 and ABI022 clinical isolate. The study indicated that this siderophore receptor can be considered for further analysis as a potential vaccine target against A. baumannii.

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“…For instance, one study has identified 35 outer membrane or extracellular adhesins, as promising A. baumannii vaccine targets. [34]. Among proteins of interest from A. baumannii, AbOmpA has recently been shown to be involved in serum resistance.…”

Section: Discussionmentioning

confidence: 99%

Comparative in silico analyses of proteins involved in serum resistance as promising vaccine candidates against Acinetobacter baumannii

Shirazi

Azizi

Bolourchi

et al. 2017

vacres

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Introduction: Acinetobacter baumannii as a Gram-negative coccobacillus has become a major cause of hospital-acquired infections. The virulence factors involved in serum resistance are important targets in the development of an effective vaccine against this pathogen. Our aim in this project was in silico analyses of A. baumannii proteins involved in serum resistance which could potentially be used as efficient vaccines. Methods: Based on computational procedures, we evaluated all A. baumannii proteins involved in serum resistance, namely AbOmpA, PKF, PLD, PBP 7/8, CipA and Tuf SurA1, as vaccine candidates. Subcellular localization, sequence conservation, domain prediction and 3D modelings were analyzed by online tools. Moreover, the prevalence of serum resistance factors in 5 strains of A. baumannii was characterized. The MHC-binding sites of class I and II were detected. Linear and conformational B cell epitopes were analyzed by 2 prediction servers. Results: The MetaLocGramN server showed that AbOmpA, PKF, PBP7/8, phospholipase D, CipA, Tuf and SurA1 were outer membrane protein (56.32%), extracellular protein (58.74%), extracellular protein (52.59%), cytoplasmic protein (45.08%), extracellular protein (53.8%), Cytoplasmic protein (96.36%) and extracellular protein (58.23%), respectively. The OMD of AbOmpA, PKF, PBP7/8 and phospholipase D, CipA, Tuf and SurA1 were 0.060, 0.076, 0.08, 0.101, 0.09, 0.06 and 0.103, respectively. The numbers of immunogenic linear and conformational epitopes with high score (P ≥ 0.6), extracted from beta-barrel of AbOmpA were 6 and 4; whereas these values for PKF were 10 and 4, respectively. Conclusion: The in silico analyses and reverse vaccinology criteria showed that AbOmpA and PKF had better attributes as vaccine targets and they could be considered as promising vaccine candidates against A. baumannii.

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Antibiotic Resistance Determinant-Focused Acinetobacter baumannii Vaccine Designed Using Reverse Vaccinology

Cited by 63 publications

References 89 publications

RETRACTED: CuO and CeO2 Nanostructures Green Synthesized Using Olive Leaf Extract Inhibits the Growth of Highly Virulent Multidrug Resistant Bacteria

RETRACTED: CuO and CeO2 Nanostructures Green Synthesized Using Olive Leaf Extract Inhibits the Growth of Highly Virulent Multidrug Resistant Bacteria

Protective potentials of a siderophore receptor against Acinetobacter baumannii infections

Comparative in silico analyses of proteins involved in serum resistance as promising vaccine candidates against Acinetobacter baumannii

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