Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon

El‐Najjar, Nahed; Farah, Maya; Hashwa, Fuad; Tokajian, Sima

doi:10.1111/j.1472-765x.2010.02926.x

Cited by 30 publications

(22 citation statements)

References 21 publications

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“…Sequencing analysis of the cassette arrays revealed a predominance of the dfrA17 and dfrA17-aadA5 arrays in this population. This finding agrees with those of previous studies reporting a strong association between class 1 integrons and the dfrA and aadA cassettes [15,33]. Indeed, the dfrA17 and aadA5 cassettes were formerly found in a class 1 integron of an E. coli isolated in 1998 from the urinary tract of an Australian patient [34].…”

Section: Molecular Characterization Of Gene Cassettessupporting

confidence: 83%

Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

Oliveira-Pinto

Diamantino

Oliveira

et al. 2017

Journal of Medical Microbiology

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Purpose. Class 1 integrons are among the main vehicles that facilitate the spread of antibiotic-resistance genes, with serious public health consequences. The aim of this cross-sectional study was to investigate the presence of class 1 integrons and to characterize their variable regions, as well as the antimicrobial resistance profiles and phylogenetic groups of a collection of Escherichia coli isolates recovered from healthy subjects (n=42) and those with urinary infection (n=40).Methodology. The methods used included PCR, sequencing and antimicrobial susceptibility testing.Results. PCR screening for the integrase gene (intI1) revealed a higher incidence of class 1 integrons in uropathogenic E. coli (65 %, UPEC) than in commensal isolates (11.9 %). Eight of 31 intI1-positive isolates, all of them UPEC, harboured empty integrons. The variable regions of the other 23 contained gene cassettes encoding resistance to b-lactams (blaOXA-1), aminoglycosides (aadA1 and aadA5), trimethoprim (dfrA1 and dfrA17) and an ORF. To our knowledge this is the first report of an ORF identified as a putative phage tail protein associated with a class 1 integron. The aadA1 and dfrA17-addA5 arrays prevailed in commensal E. coli and UPEC, respectively. UPEC isolates were highly resistant to the antimicrobials tested, in contrast to commensal isolates. The E. coli isolates carrying gene cassettes associated with class 1 integrons were found to be unrelated to any phylogroup or multiresistance.Conclusion. Co-resistance to clinically relevant fluoroquinolone and trimethoprim-sulfamethazole in all UPEC isolates is a cause for concern. These results expand the current knowledge of gene cassettes in both commensal and pathogenic E. coli.

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Section: Molecular Characterization Of Gene Cassettessupporting

confidence: 83%

Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

Oliveira-Pinto

Diamantino

Oliveira

et al. 2017

Journal of Medical Microbiology

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“…Sequencing the variable region of class 1 and 2 integrons from MDR- and XDR-UPEC strains revealed the presence of genes encoding the antibiotic modifying enzymes aad A1, aad B, aac C, ant 1, dfr A1, and dfr A17. MDR- and XDR-UPEC strains with resistance to SXT and GM antibiotics were mainly associated with class 1 and 2 integrons, as described by other authors (Solberg et al, 2006; Márquez et al, 2008; El-Najjar et al, 2010; Soleimani et al, 2014; Acosta-Pérez et al, 2015; Yahiaoui et al, 2015). Class 3 integrons are part of the soil/freshwater proteobacteria group, have a poor occurrence rate and were not identified in this study (Deng et al, 2015).…”

Section: Discussionsupporting

confidence: 63%

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

et al. 2016

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In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed and has directly impacted costs and extended hospital stays. The aim of this study was to determine MDR- and XDR-UPEC clinical strains, their virulence genes, their phylogenetic groups and to ascertain their relationship with integrons and genetic diversity. From a collection of 500 UPEC strains, 103 were selected with MDR and XDR characteristics. MDR-UPEC strains were mainly associated with phylogenetic groups D (54.87%) and B2 (39.02%) with a high percentage (≥70%) of several fimbrial genes (ecpA, fimH, csgA, and papGII), an iron uptake gene (chuA), and a toxin gene (hlyA). In addition, a moderate frequency (40–70%) of other genes (iutD, tosA, and bcsA) was observed. XDR-UPEC strains were predominantly associated with phylogenetic groups B2 (47.61%) and D (42.85%), which grouped with ≥80 virulence genes, including ecpA, fimH, csgA, papGII, iutD, and chuA. A moderate frequency (40–70%) of the tosA and hlyA genes was observed. The class 1 and 2 integrons that were identified in the MDR- and XDR-UPEC strains were associated with phylogenetic groups D, B2, and A, while the XDR-UPEC strains that were associated with phylogenetic groups B2, D, and A showed an extended-spectrum beta-lactamase (ESBL) phenotype. The modifying enzymes (aadA1, aadB, aacC, ant1, dfrA1, dfrA17, and aadA4) that were identified in the variable region of class 1 and 2 integrons from the MDR strains showed resistance to gentamycin (56.25 and 66.66%, respectively) and trimethoprim-sulfamethoxazole (84.61 and 66.66%, respectively). The MDR- and XDR-UPEC strains were distributed into seven clusters and were closely related to phylogenic groups B2 and D. The diversity analysis by PFGE showed 42.68% of clones of MDR-UPEC and no clonal association in the XDR-UPEC strains. In conclusion, phylogenetic groups including virulence genes are widely associated with two integron classes (1 and 2) in MDR- and XDR-UPEC strains.

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“…In our research, class 1 and 2 integrons were detected in 50.9% of the isolates. El-Najjar et al [26], in 2010, showed that of 100 E. coli isolates collected from patients, 30 had class 1 integrons. In our study, 24 (47.05%) of the isolates carried class 1 integrons, found in 50.9% of the MDR isolates.…”

Section: Discussionmentioning

confidence: 99%

Multidrug-Resistant <b><i>Escherichia coli</i></b> Strains Isolated from Patients Are Associated with Class 1 and 2 Integrons

Lavakhamseh¹,

Mohajeri

Rouhi

et al. 2015

Chemotherapy

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Background:Escherichia coli isolates displaying multidrug-resistance (MDR) are a major health care problem that results in mortality and morbidity. Integrons are DNA elements in E.coli that are related to antibiotic resistance. The aim of this study was to determine class 1 and 2 integrons and MDR in E. coli isolates obtained from patients in two Sanandaj hospitals, located in Iran. Materials and Methods: 120 isolates of E. coli were obtained from clinical specimens (from November 2013 to April 2014), and the susceptibility of E. coli antimicrobial agents was determined using the Kirby-Bauer disk diffusion method according to the CLSI. PCR were applied for detection of class 1 and 2 integronsinE. coliisolates. SPSS software v16 and the χ² test were used for statistical analysis in order to calculate the association between antibiotic resistance and the presence of integrons (p < 0.05). Results: In a total of 120 E. coli isolates, 42.5% had MDR. Integrons were found in 50.9% of the MDR isolates, and included 47.05% class 1 and 3.92% class 2 integrons. The strains did not have both classes of integrons simultaneously. An association between resistance to antibiotics and integrons was found. Conclusion: Our results showed that int1 and int2 genes present in E. coli isolates obtained from patients cause MDR in this isolates. Since such bacteria are a reservoir for the transmission of MDR bacteria, appropriate programs are necessary to reduce this problem.

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Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon

Cited by 30 publications

References 21 publications

Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

Multidrug-Resistant <b><i>Escherichia coli</i></b> Strains Isolated from Patients Are Associated with Class 1 and 2 Integrons

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