Antibiotic resistance with particular reference to soil microorganisms

Nwosu, Veronica C.

doi:10.1016/s0923-2508(01)01215-3

Cited by 114 publications

(61 citation statements)

References 44 publications

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“…These data indicate that the growth of transgenic plants for Ͼ10 successive years on the same field has not increased the measurable level and spectrum of antibiotic resistance in the cultivable soil bacterial fraction, thus excluding any cumulative effect related to the constant release of transgenic plant DNA. The observed multiantibiotic resistance patterns of soil communities has been reported with bacteria resisting up to eight antibiotics belonging to various other antibiotic families (13,14).…”

Section: Resultsmentioning

confidence: 91%

“…Ampicillin was deployed in 1961, and resistance was first observed 15 years later (36). Antibiotic resistance genes detected on numerous plasmids today were apparently lacking from bacterial replicons isolated in the preantibiotic era (37,38). These dramatic changes confirm the fundamental role of horizontal gene transfer (HGT) in the dispersion of these genes between bacteria.…”

Section: Bla Mutation Analysesmentioning

confidence: 98%

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Antibiotic-resistant soil bacteria in transgenic plant fields

Demanèche

Sanguin

Poté

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

149

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Understanding the prevalence and polymorphism of antibiotic resistance genes in soil bacteria and their potential to be transferred horizontally is required to evaluate the likelihood and ecological (and possibly clinical) consequences of the transfer of these genes from transgenic plants to soil bacteria. In this study, we combined culture-dependent and -independent approaches to study the prevalence and diversity of bla genes in soil bacteria and the potential impact that a 10-successive-year culture of the transgenic Bt176 corn, which has a blaTEM marker gene, could have had on the soil bacterial community. The bla gene encoding resistance to ampicillin belongs to the beta-lactam antibiotic family, which is widely used in medicine but is readily compromised by bacterial antibiotic resistance. Our results indicate that soil bacteria are naturally resistant to a broad spectrum of beta-lactam antibiotics, including the third cephalosporin generation, which has a slightly stronger discriminating effect on soil isolates than other cephalosporins. These high resistance levels for a wide range of antibiotics are partly due to the polymorphism of bla genes, which occur frequently among soil bacteria. The blaTEM116 gene of the transgenic corn Bt176 investigated here is among those frequently found, thus reducing any risk of introducing a new bacterial resistance trait from the transgenic material. In addition, no significant differences were observed in bacterial antibiotic-resistance levels between transgenic and nontransgenic corn fields, although the bacterial populations were different.antibiotic resistance ͉ GMO ͉ HGT

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Section: Resultsmentioning

confidence: 91%

Section: Bla Mutation Analysesmentioning

confidence: 98%

Antibiotic-resistant soil bacteria in transgenic plant fields

Demanèche

Sanguin

Poté

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

149

View full text Add to dashboard Cite

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“…Of recent concern is the potential development of environmental reservoirs of antibiotic resistance in farmland. Specifically, the application of untreated irrigation water or manure slurry to croplands could result in the spread of resistance to indigenous soil bacteria through horizontal transfer, which could in turn transfer resistance back to animals or humans via crops (17,21).…”

mentioning

confidence: 99%

Antimicrobial Resistance of Enterococcus Species Isolated from Produce

Johnston

Jaykus

2004

Appl Environ Microbiol

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The purpose of this study was to characterize the antibiotic resistance profiles of Enterococcus species isolated from fresh produce harvested in the southwestern United States. Among the 185 Enterococcus isolates obtained, 97 (52%) were Enterococcus faecium, 38 (21%) were Enterococcus faecalis, and 50 (27%) were other Enterococcus species. Of human clinical importance, E. faecium strains had a much higher prevalence of resistance to ciprofloxacin, tetracycline, and nitrofurantoin than E. faecalis. E. faecalis strains had a low prevalence of resistance to antibiotics used to treat E. faecalis infections of both clinical and of agricultural relevance, excluding its intrinsic resistance patterns. Thirty-four percent of the isolates had multiple-drugresistance patterns, excluding intrinsic resistance. Data on the prevalence and types of antibiotic resistance in Enterococcus species isolated from fresh produce may be used to describe baseline antibiotic susceptibility profiles associated with Enterococcus spp. isolated from the environment. The data collected may also help elucidate the role of foods in the transmission of antibiotic-resistant strains to human populations.

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“…Presently, there is only limited information available on the direct effects of these drugs on soil biota. Investigations in this field are difficult to perform because the soil microorganism community is a very complex system with at least 90% of the bacteria living in this compartment unidentified (Nwosu 2001).…”

mentioning

confidence: 99%

Antibiotics in dust originating from a pig-fattening farm: a new source of health hazard for farmers?

Hamscher

Pawelzick

Sczesny

et al. 2003

Environ Health Perspect

188

110

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Pig-house dust originates from feed, bedding, feces, and the animals themselves. If the animals receive drugs such as antibiotics, residues of these substances may occur in manure, in the air, or on surfaces of the respective animal house. In a retrospective study, we investigated dust samples collected during two decades from the same piggery for the occurrence of various antibiotics. In 90% of these samples, we detected up to five different antibiotics, including tylosin, various tetracyclines, sulfamethazine, and chloramphenicol, in total amounts up to 12.5 mg/kg dust. High dust exposure in animal confinement buildings is believed to be a respiratory health hazard because of the high content of microorganisms, endotoxins, and allergens. Further risks may arise from the inhalation of dust contaminated with a cocktail of antibiotics. Apart from that, our data provide first evidence for a new route of entry for veterinary drugs in the environment.

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Antibiotic resistance with particular reference to soil microorganisms

Cited by 114 publications

References 44 publications

Antibiotic-resistant soil bacteria in transgenic plant fields

Antibiotic-resistant soil bacteria in transgenic plant fields

Antimicrobial Resistance of Enterococcus Species Isolated from Produce

Antibiotics in dust originating from a pig-fattening farm: a new source of health hazard for farmers?

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