Antibiotic resistant pathogens versus human impacts: A study from three eco-regions of the Chennai coast, southern India

In this study, the sea water and sediment samples of four different locations were collected from Palk bay regions during 2014 for pollution indicators study. All the bacterial parameters were higher during the rainy season compared to summer season in all the sampling locations of the Palk bay regions. In the water samples, among the pollution indicator bacterial groups, TC and TS counts were highest 0.67 -10.3 [×10 3 ] ml −1 and 0.8 -10.8 [×10 2 ] ml −1 during monsoon, and the least 0.54 -8.5 [×10 3 ] ml −1 and 0.7 -9.0 [×10 2 ] ml −1 were noticed during summer. In monsoon sediment sample, the higher VC count was observed in Thondi (2100 g −1 ) followed by Mimisal (1700 g −1 ), Manamelkudi (960 g −1 ) and Devipattinam (200 g −1 ). The pollution index (PI) of each location was calculated and was showed a remarkable microbial contamination was observed in sediment sample than water sample. The order of PI observed were Thondi > Mimisal > Manamelkudi > Devipattinam. The highest PI ratio (15.5) was observed in Devipattinam sediment sample at monsoon season while lowest (2.5) was noticed in Devipattinam water sample at summer season. The results indicated that the coastal environment is highly exposed to human excreta that suggesting to avoid direct contact. Hence, throughout impoundment is needed to protect coastal environments.

Section: Resultsmentioning

confidence: 99%

Section: Bacteriological Analysismentioning

confidence: 99%

Prevalence of pollution indicators in Palk bay coastal zone, Southern India

Meera¹

2016

“…There is no antimicrobial activity in solution devoid of sample used as a vehicle control (sterile triple distilled water), reflecting that antimicrobial activity was directly related to the sample. The spherical shaped silver nanoparticles having size in range of 16-28 nm were achieved using this extract with antibacterial property observed by Kirby-Bauer method against multi-drug resistant bacteria such as Streptococcus pyogens, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Staphylococcus aureus [21]. A stable and spherical shaped silver nanoparticle was synthesized using extract of Abutilon indicum.…”

Section: Ijasr|vol 03|issue 01|2017mentioning

confidence: 99%

Bioreduction of silver nanoparticles characterizations and their antimicrobial activity

Gowthami¹,

Kanna²,

sankari³

et al. 2017

In this investigation we report the bioreduction of silver nitrate to silver nanoparticles using the leaves aqueous extract of Alangium lamarckii. The silver nanoparticles were characterized by UV-vis spectrophotometer, scanning electron microscope (SEM), DLS-Size and zeta potential analysis showed that the synthesized silver nanoparticles are varied from 60 -70 nm and have the spherical shape. Further the prospect of protein as a stabilizing material in silver nanoparticles is shown by FTIR analysis and the XRD examination confirms monocrystalline phase of silver with FCC crystal structure. The antimicrobial activity of Ag nanoparticles was investigated against some pathogens. In these assessments, silver nanoparticles (AgNP) can stop microbial growth and even kill microbes, from now confirmed their antimicrobial importance.

“…All trials were performed in triplicate. The specific biochemical tests were performed for identification (Rapid Microbial Limit Test kits used) of bacterial [15] [16]. A total of thirty (30) river bacterial isolates were challenged against three different concentrations (10, 100 and 200 mM) of copper and chromium metal salt solution for metal resistance studies by plate diffusion method.…”

Section: Multi-metal Bacteriological Analysismentioning

confidence: 99%

Assessment of multi-metal resistant bacteria from Periyar river, Southern India

Prasanth¹,

Mahesh²

2016

A total of thirty bacterial strains were isolated from the water and sediment samples of Periyar River during premonsoon 2015 for multi-metal resistant study. In this study, the bacterial isolates were challenged against three different concentrations (10, 100 and 200 mM) of copper and chromium metal salt solution by using of plate diffusion method. Most of the isolates from Periyar River were resistant to minimum concentration of the both metal solutions. Nearly, 93.3 % were resistant to copper solution whereas 90.0 % were resistant to chromium solution. In 200 mM of Cu, 46.7 % of the populations showed growth rates between 71-80%, whereas 3.3 % of the populations were observed with a 51-60 % growth rate. At 200 mM Cr, 6.7 % of the populations showed 51-60 % growth rate. A growth rate between 61-70 % was observed for 13.3 % of the bacterial populations at 200 mM of Cr, whereas no population was growing at a growth rate of 0-50 % with 200 mM of Cr. The results indicated that the river received various pollution sources throughout the river-line. The sensitivity nature of the bacterial isolates against metal solutions were varied depend on the concentrations of the metal solutions. Hence, the river needed throughout impoundment for maintaining sanitation.